Purification and properties of unicellular blue-green algae (order Chroococcales).

Stanier, Roger Y.; Kunisawa, R; Mandel, M; Cohen-Bazire, Germaine

doi:10.1128/mmbr.35.2.171-205.1971

Cited by 1,916 publications

(713 citation statements)

References 44 publications

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“…In addition, pathways for the assembly of glycogen and PHB are shown and glgC and phaA, the genes mostly used for disruption of storage compound synthesis are highlighted DAVID ET AL. | 301 Synechocystis; Stanier, Kunisawa, Mandel, & Cohen-Bazire, 1971) was obtained from the Pasteur Culture Collection of Cyanobacteria (PCC, Paris, France). BG11 medium (Stanier et al, 1971) or YBG11 medium (Shcolnick, Shaked, & Keren, 2007) was used for cultivation of Synechocystis.…”

Section: Cultivation Of Strainsmentioning

confidence: 99%

“…| 301 Synechocystis; Stanier, Kunisawa, Mandel, & Cohen-Bazire, 1971) was obtained from the Pasteur Culture Collection of Cyanobacteria (PCC, Paris, France). BG11 medium (Stanier et al, 1971) or YBG11 medium (Shcolnick, Shaked, & Keren, 2007) was used for cultivation of Synechocystis. Agar plates containing the respective antibiotics (either Km 50 , Cm 15 , or a combination of both, where the superscript refers to the respective antibiotic concentration in μg ml −1 ) were inoculated from a cryo stock, and cultivated for 10 days under 25 μmol m −2 s −1 photosynthetically active radiation, ambient CO 2 , 30°C, and 75% humidity in a polyklima incubator (Polyklima, Freising, Germany…”

Section: Cultivation Of Strainsmentioning

confidence: 99%

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Production of 1,2‐propanediol in photoautotrophic Synechocystis is linked to glycogen turn‐over

David

Schmid

Adrian

et al. 2017

Biotech & Bioengineering

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We utilized a photoautotrophic organism to synthesize 1,2-propanediol from carbon dioxide and water fueled by light. A synthetic pathway comprising mgsA (methylglyoxal synthase), yqhD (aldehyde reductase), and adh (alcohol dehydrogenase) was inserted into Synechocystis sp. PCC6803 to convert dihydroxyacetone phosphate to methylglyoxal, which is subsequently reduced to acetol and then to 1,2-propanediol. 1,2-propanediol could be successfully produced by Synechocystis, at an approximate rate of 55 μmol h g . Surprisingly, maximal productivity was observed in the stationary phase. The production of 1,2-propanediol was clearly coupled to the turn-over of intracellular glycogen. Upon depletion of the glycogen pool, product formation stopped. Reducing the carbon flux to glycogen significantly decreased final product titers. Optimization of cultivation conditions allowed final product titers of almost 1 g L (12 mM), which belongs to the highest values published so far for photoautotrophic production of this compound.

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Section: Cultivation Of Strainsmentioning

confidence: 99%

Section: Cultivation Of Strainsmentioning

confidence: 99%

Production of 1,2‐propanediol in photoautotrophic Synechocystis is linked to glycogen turn‐over

David

Schmid

Adrian

et al. 2017

Biotech & Bioengineering

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“…All Synechocystis cultures were grown on liquid BG-11 supplemented with 10 mM TES-NaOH, under constant light (100 mE 610%) at 308C at 225 rpm under ambient CO 2 concentrations, unless otherwise stated. 22 All E. coli DH5a cells (Invitrogen, Grand Island, NY) cells were grown in Luria-Bertani (LB) Medium at 378 C and 225 rpm. All cultures tested in diel-cycle light conditions were allowed to grow for two full 12 hour light/12 hour dark cycles prior to sampling.…”

Section: Strains and Growth Conditionsmentioning

confidence: 99%

Evaluating Light‐Induced Promoters for the Control of Heterologous Gene Expression in Synechocystis sp. PCC 6803

Albers

Peebles

2016

Biotechnology Progress

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Cyanobacteria are enticing microbial factories, but little is understood how their gene control elements respond to the periodic availability to light. This research tested the capability of P to control gene expression during light/dark conditions when moved to a neutral location within the Synechocystis sp. PCC 6803 genome. When the eYFP reporter gene was run by P in the promoter's native genomic location, mutants exposed to 12-hour light conditions experienced a 15.8× increase in transcript abundance over that observed from the same construct exposed to 12-hour dark conditions. When this same construct was moved to the hypothetical coding region slr0168 in the genome, transcripts generated during 12 hour light conditions accumulated to 1.67X of the levels of transcripts generated by the same construct during 12 hour dark conditions. Three additional promoter constructs, P , P , and P were also tested for differential expression in light and dark conditions within the neutral region slr0168. While low amounts of transcript accumulation were observed from P and P , the P construct accumulated 5.79× more transcripts when compared to transcript abundance during dark conditions, which highlights the potential of this promoter to control gene expression during diel-cycle light conditions. Additionally, nucleotide mutations were made to regions within P . Mutations to the cis-acting hexo-nucleotide region increased expression 3.71× over that of the native promoter, while the addition of the "HLR" nucleotide region to the P construct increased expression 2.76× over that of the native promoter. © 2016 American Institute of Chemical Engineers Biotechnol. Prog., 33:45-53, 2017.

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“…The cultures were grown on shakers (100 r.p.m.) in flasks containing medium BG11 (Stanier et al, 1971) supplemented with 20 mM TAPS-NaOH, pH = 9 and 10 mM NaHCO3. Light intensity was 10-15 mmol photons m -2 s -1 provided by cool white fluorescent lamps and the temperature was 30°C.…”

Section: Strain Isolation and Growth Conditionsmentioning

confidence: 99%

An AbrB‐like protein might be involved in the regulation of cylindrospermopsin production by Aphanizomenon ovalisporum

Shalev-Malul

Lieman-Hurwitz

Viner-Mozzini

et al. 2008

Environmental Microbiology

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Certain filamentous cyanobacteria, including Aphanizomenon ovalisporum, are potentially toxic owing to the formation of the hepatotoxin cylindrospermopsin. We previously identified a gene cluster in A. ovalisporum likely to be involved in cylindrospermopsin biosynthesis, including amidinotransferase (aoaA) and polyketide-synthase (aoaC), transcribed on the reverse strands. Analysis of the genomic region between aoaA and aoaC identified two transcription start points for each of these genes, differentially expressed under nitrogen and light stress conditions. The transcript abundances of these genes and the cylindrospermopsin level were both affected by nitrogen availability and light intensity. Gel shift assays and DNA affinity columns isolated a protein that specifically binds to a 150 bp DNA fragment from the region between aoaA and aoaC, and MS/MS analyses identified similarity to AbrB in other cyanobacteria and in Bacillus sp. Comparison of the native AbrB isolated from A. ovalisporum with that obtained after cloning and overexpression of abrB in Escherichia coli identified specific post-translational modifications in the native cyanobacterial protein. These modifications, which are missing in the protein expressed in E. coli, include N-acetylation and methylation of specific residues. We discuss the possible role of these modifications in the regulation of cylindrospermopsin production in Aphanizomenon.

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Purification and properties of unicellular blue-green algae (order Chroococcales).

Cited by 1,916 publications

References 44 publications

Production of 1,2‐propanediol in photoautotrophic Synechocystis is linked to glycogen turn‐over

Production of 1,2‐propanediol in photoautotrophic Synechocystis is linked to glycogen turn‐over

Evaluating Light‐Induced Promoters for the Control of Heterologous Gene Expression in Synechocystis sp. PCC 6803

An AbrB‐like protein might be involved in the regulation of cylindrospermopsin production by Aphanizomenon ovalisporum

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