Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants

Simkovich, Aaron; Kohalmi, Susanne E.; Wang, Aiming

doi:10.1007/978-1-0716-1835-6_13

Cited by 1 publication

(1 citation statement)

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“…In particular, combined multi-omics analytical methods are widely used in the study of plant–microbial interactions [ 18 , 19 ]. In terms of the functional research related to peanuts, transcriptome and proteome sequencing were used to assist in screening the key genes involved in aerial and subterranean pods [ 20 ], drought resistance [ 21 , 22 ], development [ 23 , 24 ], environmental stress [ 25 ], and interaction with Aspergillus flavus [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Transcriptomic–Proteomic Analysis Revealed the Regulatory Mechanism of Peanut in Response to Fusarium oxysporum

Wang,

Zhu,

Zhang

et al. 2024

IJMS

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Peanut Fusarium rot, which is widely observed in the main peanut-producing areas in China, has become a significant factor that has limited the yield and quality in recent years. It is highly urgent and significant to clarify the regulatory mechanism of peanuts in response to Fusarium oxysporum. In this study, transcriptome and proteome profiling were combined to provide new insights into the molecular mechanisms of peanut stems after F. oxysporums infection. A total of 3746 differentially expressed genes (DEGs) and 305 differentially expressed proteins (DEPs) were screened. The upregulated DEGs and DEPs were primarily enriched in flavonoid biosynthesis, circadian rhythm-plant, and plant–pathogen interaction pathways. Then, qRT-PCR analysis revealed that the expression levels of phenylalanine ammonia-lyase (PAL), chalcone isomerase (CHI), and cinnamic acid-4-hydroxylase (C4H) genes increased after F. oxysporums infection. Moreover, the expressions of these genes varied in different peanut tissues. All the results revealed that many metabolic pathways in peanut were activated by improving key gene expressions and the contents of key enzymes, which play critical roles in preventing fungi infection. Importantly, this research provides the foundation of biological and chemical analysis for peanut disease resistance mechanisms.

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