Purification and Reconstitution of the Ryanodine- and Caffeine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum

Meissner, Gerhard; Lai, F. Anthony; Anderson, Kristin A.; Xu, Le; Liu, Qi-Yi; Herrmann‐Frank, Annegret; Rousseau, Éric; Jones, Rodney V.; Lee, Hee-Bong

doi:10.1007/978-1-4684-6003-2_20

Cited by 16 publications

(9 citation statements)

References 39 publications

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“…2C), which appeared to be identical to the previous result (30). Caffeine has been shown to be an activator of the ryanodine receptor in the sarcoplasmic reticulum (31,32). When caffeine was applied to ryanodine receptor cDNA-transfected cells, caffeine-sensitive Ca 2ϩ release was observed in one cell at the periphery of cell clusters, which appeared to be ryanodine receptor-expressing cells (Fig.…”

Section: Expression Of Transfected Connexin-43 and The Ryanodinesupporting

confidence: 84%

“…[Ca 2ϩ ] i was calculated as the ratio of the fluorescence intensities at 340 and 380 nm at 2-s intervals. Application of caffeine, an activator of the ryanodine receptor (31,32), was performed by replacing the control HEPES buffer with the same buffer containing 15 mM caffeine, during which maps of [Ca 2ϩ ] i in all cells in frame were obtained using the image processor.…”

Section: Construction Of Mutant Connexin-43s-mentioning

confidence: 99%

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Intercellular Calcium Signaling via Gap Junction in Connexin-43-transfected Cells

Toyofuku

Yabuki

Otsu

et al. 1998

Journal of Biological Chemistry

101

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Section: Expression Of Transfected Connexin-43 and The Ryanodinesupporting

confidence: 84%

Section: Construction Of Mutant Connexin-43s-mentioning

confidence: 99%

Intercellular Calcium Signaling via Gap Junction in Connexin-43-transfected Cells

Toyofuku

Yabuki

Otsu

et al. 1998

Journal of Biological Chemistry

101

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“…These vesicles were used for [ 3 H]ryanodine displacement binding affinity assays to determine the affinity of ryanoids for RyR2. Junctional sarcoplasmic reticular membrane vesicles were prepared in the presence of the protease inhibitors, using discontinuous sucrose gradients as described previously (23). Junctional sarcoplasmic reticular membrane vesicles were solubilized with CHAPS and 30 S RyR2 complexes were isolated by rate density gradient centrifugation and reconstituted into proteoliposomes (24).…”

Section: Methodsmentioning

confidence: 99%

Diketopyridylryanodine Has Three Concentration-dependent Effects on the Cardiac Calcium-release Channel/Ryanodine Receptor

Bidasee¹,

Xu²,

Meissner³

et al. 2003

Journal of Biological Chemistry

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By interacting with more than one site, ryanoids induce multiple effects on calcium-release channels. To date, the kinetics of interaction of only one of these sites has been characterized. Using C(4),C(12)-diketopyridylryanodine in both [(3)H]ryanodine binding and single channel experiments we characterized another site on the cardiac ryanodine receptor (RyR2) with which ryanoids interact. Competitive binding of this ryanoid to RyR2 implied a minimal two-site binding model. At the single channel level, C(4),C(12)-diketopyridylryanodine induced three distinct effects. At nanomolar concentrations, it increased channel open probability severalfold without inducing a subconductance. This effect was independent of membrane holding potential. As for other ryanoids, low micromolar concentrations of C(4),C(12)-diketopyridylryanodine readily induced a subconductance state. The major subconductance had a current amplitude of 52% of fully open, it was reversible, and its time to induction and duration were voltage- and concentration-dependent, affording Hill slopes of >2. At higher micromolar concentrations C(4),C(12)-diketopyridylryanodine induced long lasting, yet reversible shut states. Using a pharmacological strategy we have discerned an additional ryanoid-binding site on RyR2 that triggers an increase in channel activity. This site likely resides outside the strict confines of the transmembrane conducting pathway.

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“…Thapsigargin, a naturally occurring sesquiterpene lactone isolated from the umbelliferous plant Thapsia garganica (Rasmussen et al, 1978), was used to deplete IP,-dependent stores by selectively blocking a Ca-ATPase pump located in the smooth endoplasmic reticulum (SER) (Thastrup et al, 1989a,b;Verma et al, 1990). Once these pools are depleted, IP, generation does not result in calcium transients, whereas calcium-dependent calcium release stores may be activated by application of caffeine and can be blocked by ryanodine (Meissner et al, 1991). These experimental paradigms were applied to determine if intracellular calcium stores are involved in the axonal activity-induced…”

Section: Intracellular Calcium Stores In Myelinating Schwann Cellsmentioning

confidence: 99%

Axonal activation-induced calcium transients in myelinating Schwann cells, sources, and mechanisms

Lev‐Ram

Ellisman

1995

J. Neurosci.

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We have investigated the role of myelinating glia in events associated with propagation of the action potential at nodes of Ranvier using combinations of optical and electrophysiological recording methods. Calcium transients were observed in Schwann cells by fluorescent imaging of the nodal complex of fibers loaded with the calcium-sensitive dye fluo3-AM. To follow [Ca2+]i changes associated with neuronal activity at the node of Ranvier, nerves loaded with fluo3 were imaged during axonal activation using laser-scanning confocal microscopy. To elucidate sources of [Ca2+]i transients, we tested the effects of drugs known to alter [Ca2+]i. [Ca2+]i transients in Schwann cells were observed in response to axonal activation and these were subsequently blocked by ryanodine if ryanodine was present during a previous [Ca2+]i transient. Bath applications of caffeine induced [Ca2+]i transients which could be blocked by ryanodine. These findings indicate that calcium-activated calcium release occurs in Schwann cells in response to impulse activity.

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Purification and Reconstitution of the Ryanodine- and Caffeine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum

Cited by 16 publications

References 39 publications

Intercellular Calcium Signaling via Gap Junction in Connexin-43-transfected Cells

Intercellular Calcium Signaling via Gap Junction in Connexin-43-transfected Cells

Diketopyridylryanodine Has Three Concentration-dependent Effects on the Cardiac Calcium-release Channel/Ryanodine Receptor

Axonal activation-induced calcium transients in myelinating Schwann cells, sources, and mechanisms

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