Purification and Serological Characterization of Three Basic 15-Kilodalton Pathogenesis-Related Proteins from Tomato

Joosten, M.H.A.J.; Bergmans, Cécile J. B.; Meulenhoff, E. Josien S.; Cornelissen, Ben J. C.; Wit, P.J.G.M. de

doi:10.1104/pp.94.2.585

Cited by 53 publications

(49 citation statements)

References 30 publications

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“…Comparison of the electrophoretic patterns under native conditions described in the present study (Fig. 4B) to those reported by Joosten et al (1990) suggests that (a) our major protein, P14a, corresponds to P6 and is indeed the most prominent of the 15-kD proteins; (b) our P14b corresponds to P4; and (c) our P14c was not detected by Joosten et al (1990), since it migrates only slightly faster than P14a on native acidic gels and is separated from P14a upon cationexchange chromatography (see Fig. 3A, fractions 11 and 13) only with the use of a highly resolving NaCl gradient.…”

Section: Discussionsupporting

confidence: 72%

“…It was shown later that after infection with virulent or avirulent races of Cladosporium fulvum the pattern of induction of tomato basic 14-to 15-kD proteins was more complicated, and three distinct proteins named P2, P4, and P6 were purified (Joosten et al, 1990). The authors obtained evidence that P2 was related to the PR-4 family of tobacco PR proteins, whereas P4 and P6 were related to the PR-1 family.…”

Section: Discussionmentioning

confidence: 99%

“…It was purified and shown to be similar to the tobacco PR-1 proteins (Camacho Henriquez and Sanger, 1984;Lucas et al, 1985;Cornelissen et al, 1986). It was found later that the pattern of tomato PR proteins in the 15-kD range was more complicated than initially described, at least during the response to infection by Cladosporium fulvum (Joosten et al, 1990).…”

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confidence: 95%

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Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Niderman¹,

Genetet²,

Bruyère³

et al. 1995

Plant Physiol.

394

193

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Three distinct basic 14-kD proteins, P14a, P14b, and P14c, were isolated from tomato (Lycopersicon esculentum Mill. cv Baby) leaves infected with Phytophthora infestans. They exhibited antifungal activity against P. infestam both in vitro (inhibition of zoospore germination) and in vivo with a tomato leaf disc assay (decrease in infected leaf surface). Serological cross-reactions and amino acid sequence comparisons showed that the three proteins are members of the PR-1 group of pathogenesis-related (PR) proteins. P14a and P14b showed high similarity to a previously characterized P14, whereas P14c was found to be very similar to a putative basic-type PR-1 from tobacco predicted from isolated DNA clones. This protein, named PR-1 g, was purified from virus-infected tobacco (Nicotiana tabacum Samsun NN) leaves and characterized by amino acid microsequencing, along with the well-known acidic tobacco PR-la, PR-lb, and PR-lc. l h e various tomato and tobacco PR-1 proteins were compared for their biological activity and found to display differential fungicidal activity against P. infestam in both the in vitro and in vivo assays, the most efficient being the newly characterized tomato P14c and tobacco PR-1 g.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

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Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Niderman¹,

Genetet²,

Bruyère³

et al. 1995

Plant Physiol.

394

193

View full text Add to dashboard Cite

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“…PRs are well known as major constituents of the antimicrobial cocktails that plants produce in response to pathogen attack. They usually have the typical N-terminal signal peptide for secretion and mainly accumulate in the apoplast (Joosten et al, 1990;Van Loon and Van Strien, 1999). Previous studies already indicated that the vesicle-trafficking machinery downstream of the exocyst is important for the focal secretion of PR-1.…”

Section: Discussionmentioning

confidence: 99%

Phytophthora infestans RXLR effector AVR1 interacts with exocyst component Sec5 to manipulate plant immunity

et al. 2015

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Phytophthora infestans secretes numerous RXLR effectors that modulate host defense and thereby pave the way for successful invasion. Here, we show that the RXLR effector AVR1 is a virulence factor that promotes colonization and suppresses callose deposition, a hallmark of basal defense. To identify host targets of AVR1, we performed yeast two-hybrid screens and selected Sec5 as a candidate. Sec5 is a subunit of the exocyst, a protein complex that is involved in vesicle trafficking. AVR1-like (A-L), a close homolog of AVR1, also acts as a virulence factor, but unlike AVR1, A-L does not suppress CRINKLER2 (CRN2)-induced cell death or interact with Sec5. Compared with AVR1, A-L is shorter and lacks the carboxyl-terminal tail, the T-region that is crucial for CRN2-induced cell death suppression and Sec5 interaction. In planta analyses revealed that AVR1 and Sec5 are in close proximity, and coimmunoprecipitation confirmed the interaction. Sec5 is required for secretion of the pathogenesis-related protein PR-1 and callose deposition and also plays a role in CRN2-induced cell death. Our findings show that P. infestans manipulates an exocyst subunit and thereby potentially disturbs vesicle trafficking, a cellular process that is important for basal defense. This is a novel strategy that oomycete pathogens exploit to modulate host defense.

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“…Intracellular basis chitinases were extracted as described previously (Joosten et al, 1990(Joosten et al, , 1995. A 50-mL aliquot of Trichoderma viride spores was incubated overnight at room temperature at a concentration of 100 conidia mL 21 .…”

Section: Cell Protection Assays Using Crude Extract Of Chitinase Frommentioning

confidence: 99%

Effector-Mediated Suppression of Chitin-Triggered Immunity by Magnaporthe oryzae Is Necessary for Rice Blast Disease

et al. 2012

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Plants use pattern recognition receptors to defend themselves from microbial pathogens. These receptors recognize pathogen-associated molecular patterns (PAMPs) and activate signaling pathways that lead to immunity. In rice (Oryza sativa), the chitin elicitor binding protein (CEBiP) recognizes chitin oligosaccharides released from the cell walls of fungal pathogens. Here, we show that the rice blast fungus Magnaporthe oryzae overcomes this first line of plant defense by secreting an effector protein, Secreted LysM Protein1 (Slp1), during invasion of new rice cells. We demonstrate that Slp1 accumulates at the interface between the fungal cell wall and the rice plasma membrane, can bind to chitin, and is able to suppress chitin-induced plant immune responses, including generation of reactive oxygen species and plant defense gene expression. Furthermore, we show that Slp1 competes with CEBiP for binding of chitin oligosaccharides. Slp1 is required by M. oryzae for full virulence and exerts a significant effect on tissue invasion and disease lesion expansion. By contrast, gene silencing of CEBiP in rice allows M. oryzae to cause rice blast disease in the absence of Slp1. We propose that Slp1 sequesters chitin oligosaccharides to prevent PAMP-triggered immunity in rice, thereby facilitating rapid spread of the fungus within host tissue.

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Purification and Serological Characterization of Three Basic 15-Kilodalton Pathogenesis-Related Proteins from Tomato

Cited by 53 publications

References 30 publications

Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Pathogenesis-Related PR-1 Proteins Are Antifungal (Isolation and Characterization of Three 14-Kilodalton Proteins of Tomato and of a Basic PR-1 of Tobacco with Inhibitory Activity against Phytophthora infestans)

Phytophthora infestans RXLR effector AVR1 interacts with exocyst component Sec5 to manipulate plant immunity

Effector-Mediated Suppression of Chitin-Triggered Immunity by Magnaporthe oryzae Is Necessary for Rice Blast Disease

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