This review describes studies on ensuring the microbiological safety of food. These studies con tribute to the processing and storage of high-quality and highly safe foods. The studies are focused on these areas of investigation: 1) simple and rapid methods for detecting and identifying various foodborne pathogens and bacterial toxins; 2) scientific evidence at the basis of hygiene management for the production of agricultural produce, such as food poisoning bacterial contamination from the cultivation environment of produce that are raw materials for food products and the survival of pathogens on the produce; 3) effective decontamination methods through the combination of treatment with detergents and sterilizers for agricultural produce; and 4) elucidating the mechanisms of action of food ingredients on growth inhibition of vegetative cells and spores, on activity of bacterial toxin, and on inhibition of biofilm formation of foodborne pathogens that are the basis for the development of safe storage technologies for food. The findings of these studies contribute to improving the microbiological safety of foods, which leads to the development of food science and technology and the food industry.