Purification and Some Properties of An Aldehyde Oxidase from Streptomyces Rimosus ATCC10970

Abstract

An aldehyde oxidase was purified from a cell-free extract of Streptomyces rimosus ATCC10970 to an electrophoretically homogeneous state. The molecular mass of the native enzyme was estimated to be 150 kDa by a gel filtration. SDS-polyacryamide gel electrophoresis showed that the enzyme consisted of three non-identical subunits with molecular masses of 79, 39 and 23 kDa. The absorption spectrum revealed a distinctive feature as an enzyme belonging to the xanthine oxidase family with maxima at 277, 325, 365, 415, 450, 480, and 550 nm. A variety of aliphatic and aromatic aldehydes were oxidized, but nitrogen-containing heterocyclic compounds were not. Among the substrates tested, n-heptanal was most rapidly acted on. Its optimum pH and temperature were pH 7.0 and 30°C, respectively.

A Novel Microbial Aldehyde Oxidase Applicable to Production of Useful Raw Materials, Glycolic Acid and Glyoxylic Acid, from Ethylene Glycol

“…KY4690 [5], Pseudomonas stuzeri IFO12695 [6], Methylobacillus sp. KY4400 [7], and Streptomyces rimosus ATCC10970 [8] (Table 1). However, their N-terminal amino acid sequences were not similar to those of the BurkALOD, although the 39 kDa-subunit of ALOD from Pseudomonas sp.…”