Purification and some Properties of an Italian Isolate of Poplar Mosaic Virus

Luisoni, E.; Boccardo, G.; Milne, Robert G.

doi:10.1111/j.1439-0434.1976.tb04801.x

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…As recently reviewed by Luisoni et al (1976), poplar mosaic virus (PMV) is widespread in Europe. In the Netherlands the virus has been studied by Berg (1964).…”

Section: Introductionmentioning

confidence: 99%

“…In the Netherlands the virus has been studied by Berg (1964). Properties and morphology of its particles (Biddle and Tinsley, 1971a;Boccardo and Milne, 1976) place PMV in the carlavirus group (Fenner, 1976), although aphid transmission is uncertain, and serological relationships with recognized members of the carlavirus group could not be detected (Biddle and Tinsley, 1971c). Electron-microscopical studies of the virus in ultra-thin sections also indicate that PMV belongs to the carlavirus group (Atkinson and Cooper, 1976;Brunt et al, 1976;Boccardo and Milne, 1976).…”

Section: Introductionmentioning

confidence: 99%

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Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

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Poplar mosaic virus (PMV) was purified from Nicotiana megalosiphon or N. clevelandii and antisera with titres from 256 to 4096 were prepared. One of these was used for the detection of PMV in poplar stools with the enzyme-linked immunosorbent assay (ELISA). Although ELISA was less sensitive than the infectivity test on N. megalosiphon, both tests were equally reliable when using lower leaves in July and August, and when applied to tip leaves at the end of the growing season, With both tests more infected trees were detected than with visual inspection. For PMV a particle length of 661 nm was calculated. AddressInstituut voor Plantenziektenkundig Onderzoek, Binnenhaven 12, Postbus 42, 6700 AA Wageningen.

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“…As recently reviewed by Luisoni et al (1976), poplar mosaic virus (PMV) is widespread in Europe. In the Netherlands the virus has been studied by Berg (1964).…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

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“…The concentration of the virus however was low in the present purification, which may be attributed to the presence of fibrous matrix of the host tissue which interferes with the various stages of purification of the virus which is one of the major drawbacks encountered in many potyvirus purification procedures [14], who has also reported aggregation of virus particles at early stages of purification of CdMV. Similar problems in purification of potyviruses has been reported in Lettuce Mosaic Virus [15], Soybean Mosaic Virus [16], Potato Virus Y [17], Tobacco Etch Virus [18], Pea Seed-borne Mosaic Virus [18], Maize Dwarf Virus [20], and Poplar Mosaic Virus [21]. To avoid this, detergents such as Triton X-100 was used which disrupt the particles of elongated viruses that tend to aggregate and bind to plant organelles.…”

Section: Results and Discussion Virus Purification And Electron Micromentioning

confidence: 87%

Serological Detection of Cardamom Mosaic Virus Infecting Small Cardamom, Elettaria cardamomum L

Tiwari¹,

Peter²,

Phadnis³

2016

IJLSSR

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ABSTRACT-Small Cardamom (Elettariacardamomum L. Maton) is one of the major spice crops of India, which were the world's largest producer and exporter of cardamom till 1980. There has however been a reduction in production, mainly because of Katte disease, caused by cardamom mosaic virus (CdMV) a potyvirus. Viral diseases can be managed effectively by early diagnosis using serological methods. In the present investigation, CdMV isolates were sampled from Mudigere, Karnataka, ultra purified, and electron micro graphed for confirmation. Polyclonal antibodies were raised against the virus and a direct antigen coating plate Enzyme linked immunosorbent Assay (DAC-ELISA) and Dot-ELISA (DIBA) standardized to detect the virus in diseased and tissue cultured plants. Early diagnosis in planting material will aid in using disease free material for better yields and hence increased profit to the farmer.

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“…The distant serological relationship with shallot latent virus (Bos et al, 1978) links LLV with other viruses of the carlavirus group, while the serological relationship between LLV and PMV provides the hitherto missing link between PMV and other carlaviruses (Luisoni et al, 1976).…”

Section: Discussionmentioning

confidence: 99%

Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

Meer¹,

Maat²,

Vink³

1980

Netherlands Journal of Plant Pathology

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A virus with elongate particles (656 nm) was isolated from several Lonicera species. This virus, apparently belonging to the carlavirus group, is serologically distantly related to shallot latent virus and closely related to poplar mosaic virus. The inability to infect poplar and two other hosts of poplar mosaic virus characterizes the virus from Lonicera as a new virus which was named Lonicera latent virus.The virus was easily sap-transmissible but was not transmitted by Myzuspersicae. Dilution end-point was about 10-3, thermal inactivation between 65 ~ and 80 ~ and ageing in vitro 1-6 days.Heat treatment, combined with tip-rooting appeared to be a good method to eliminate the virus from several Lonicera species and cultivars.Additional keywords." Nicotiana megalosiphon, virus adaptation, yellow vein mosaic of Lonicera japonica. AddressInstituut voor Plantenziektenkundig Onderzoek,

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Purification and some Properties of an Italian Isolate of Poplar Mosaic Virus

Cited by 14 publications

References 12 publications

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Poplar mosaic virus: purification, antiserum preparation, and detection in poplars with the enzyme-linked immunosorbent assay (ELISA) and with infectivity tests on Nicotiana megalosiphon

Serological Detection of Cardamom Mosaic Virus Infecting Small Cardamom, Elettaria cardamomum L

Lonicera latent virus, a new carlavirus serologically related to poplar mosaic virus: some properties and inactivation in vivo by heat treatment

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