Purification and stability of specifically tritium labelled lysine vasopressin (Tyrosine2-3-T-lysine8-vasopressin)

Carlsson, Lars; Pliška, V.; Thorn, Niels A.

doi:10.1007/bf01897604

Cited by 8 publications

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“…As demonstrated by Carlsson et al (1969) the material loses its purity on standing. Before use it was purified on a CM Sephadex C 25 column and eluted under starting conditions with 0.5 m ammonium acetate.…”

Section: Swedenmentioning

confidence: 99%

Uptake of Tritiated Lysine-Vasopressin by Isolated Secretory Granules From Bovine Neurohypophyses

Vilhardt

1971

Acta Endocrinologica

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A subcellular fraction containing neurosecretory granules was obtained by differential centrifugation of homogenized neural lobes of bovine hypophyses. The isolated granules were incubated with tritium labelled lysine-vasopressin and the uptake of the radioactive hormone by the granules was studied. Since enzymes capable of breaking down vasopressin were present in the fraction, it was necessary after the incubation to separate the labelled vasopressin from its radioactive breakdown products. This was done by paper chromatography. The results showed that vasopressin readily reached a higher concentration in the granules than in the surrounding medium. This process was not changed by cold, N2 or metabolic inhibitors. During the incubation radioactivity soon appeared in the proteins of the granules.

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Section: Swedenmentioning

confidence: 99%