Purification and stabilization of 99mTc-d,l-HMPAO: role of organic extractants

Chattopadhyay, Sankha; Das, Manab Kumar; Vanaja, R.; Ramamoorthy, N.

doi:10.1016/s0969-8051(01)00235-9

Cited by 9 publications

(4 citation statements)

References 7 publications

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“…There are limited studies about milk proteins labeled with a radionuclide in the literature. One of them is about combining of radiolabeled whey proteins into casein micelles by heat processing published by Noh and Richardson [19][20][21][22][23][24][25][26][27][28][29]. In this study, WH was labeled with 14 C then radioactivities of the WH and radioacti-vities of the washed casein pellets from renneted skim milk were measured.…”

Section: Resultsmentioning

confidence: 99%

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Akinli

Tekin

Müftüler

et al. 2021

Hacettepe Journal of Biology and Chemistry

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B u çalışmada Whey proteini kalay klorür indirgenme metodu kullanılarak Tc-99m ile işaretlenmiştir. Radyokromatografi çalışmalarında kağıt elektroforezi tekniği kullanılmıştır. Uygun hareketli faz olarak SF (serum fizyolojik) kullanılmıştır. Aynı zamanda Tc-99m ile işaretli Whey protein'in bağlanma verimi yaklaşık %95 olarak bulunmuştur. Daha sonra, sırasıyla lipofilite ve stabilite çalışmaları gerçekleştirilmiştir. Son olarak Albino Wistar sıçanlarda görüntüleme ve biyodağılım çalışmaları yapılmıştır. Sonuç olarak, bütün elde edilen sonuçlar Tc-99m ile işaretli Whey protein'in gelecekte meme, yumurtalık ve pankreas kanserlerinin teşhisinde kullanılabileceğini göstermiştir.

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Section: Resultsmentioning

confidence: 99%

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Akinli

Tekin

Müftüler

et al. 2021

Hacettepe Journal of Biology and Chemistry

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“…5 The aim of this paper is to standardize the quality control methods of Tc-ENS and to study the stability of this radiopharmaceutical.…”

Section: Discussionmentioning

confidence: 99%

Quality control methods for ^99mTc‐labeled exogenous natural surfactant (^99mTc‐ENS)

Calmanovici

Ricardo

Goldman

et al. 2003

Labelled Comp Radiopharmac

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SummaryTo standardize the quality control for 99m Tc-ENS, the following methods were studied: (1) physical properties and pH, (2) radiochemical purity (chromatographic studies on Whatman-1 paper, or instant thin-layer chromatography and solvent extraction using different solvents and (3) rat biodistribution studies by intratracheal injection. The tolerance limits were fixed for each method. The radiopharmaceutical stability was also evaluated. The results showed that 99m Tc-ENS was a white suspension with a pH between 4.0 and 6.0. The limit for radiochemical impurities in Whatman-1 paper/acetone was fixed at lower than 2% and the established limit for the organic aliquot in cyclohexane extraction was greater than 2%. In the biodistribution studies, the limits for activity concentration were fixed at greater than 90% for lungs, less than 9% for the gastrointestinal system and less than 1% for the sum of the other organs studied. After a storage time of 6 h at room temperature or in a refrigerator, 99mTc-ENS physical properties and pH, radiochemical and biodistribution results were within the established values. In conclusion, the quality control methods for 99m Tc-ENS are tests on physical properties and pH, radiochemical purity by Whatman-1 paper/acetone chromatography and cyclohexane extraction and biodistribution studies in rats. The stability of this radiopharmaceutical is at least 6 h at room temperature.

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“…Many attempts have been made to improve the utilization of labelled product, some have been aimed at fractionating a reconstituted vial, others have been sought to overcome the restrictions on eluate, but most of the works were focused on stretching the shelf life by addition of chelating agents, oxidizing agents, free radical scavengers or pH buffers . Favourable results have been achieved with cobalt chloride, resulting in a new commercial kit with 6 h of stability but not indicated for cell labelling…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4]6,7 Many attempts have been made to improve the utilization of labelled product, some have been aimed at fractionating a reconstituted vial, 6,8,9 others have been sought to overcome the restrictions on eluate, 3 but most of the works were focused on stretching the shelf life by addition of chelating agents, oxidizing agents, free radical scavengers or pH buffers. 3,[10][11][12][13][14][15] Favourable results have been achieved with cobalt chloride, resulting in a new commercial kit with 6 h of stability but not indicated for cell labelling. 4,16 It has been reported that HMPAO is more sensitive to radiolysis than other 99m Tc radiopharmaceuticals, and it can be inferred that self irradiation plays an important role in the molecule stability, because of the free radicals produced from the decay of 99m Tc.…”

mentioning

confidence: 99%

Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

Martínez

Miñana

Martín-Falquina

et al. 2013

Labelled Comp Radiopharmac

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99mTc-d,l-hexamethylpropylene amine oxime ((99m) Tc-d,l-HMPAO) is a widely used radiopharmaceutical that suffers from an inherent instability with a shelf life of 30 min that constrains its availability for clinical use. A protocol for improving the stability of the kit with minimal modification of manufacturer's instructions and no chemicals addition to the commercial formulation is proposed. The protocol is based on the displacement of the oxygen present in the preparation, preventing free radicals build up and free pertechnetate formation. Although the degradation of (99m) Tc-d,l-HMPAO cannot be explained solely by the radiolytic production of free radicals, it appears to be an important factor in the shelf stability of the complex.

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Purification and stabilization of 99mTc-d,l-HMPAO: role of organic extractants

Cited by 9 publications

References 7 publications

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Quality control methods for ^99mTc‐labeled exogenous natural surfactant (^99mTc‐ENS)

Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

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Purification and stabilization of 99mTc-d,l-HMPAO: role of organic extractants

Cited by 9 publications

References 7 publications

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Tc-99m ile İşaretli Whey Proteinin Deney Hayvanlarındaki Biyodağılımı

Quality control methods for 99mTc‐labeled exogenous natural surfactant (99mTc‐ENS)

Influence of radiolytic‐produced hydrogen peroxide on the stability of commercial kit 99mtechnetium‐exametazime preparation

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Product

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Quality control methods for ^99mTc‐labeled exogenous natural surfactant (^99mTc‐ENS)