Purification and Structural Characterization of Dendrobium officinale Polysaccharides and Its Activities

Abstract

In this study, Dendrobium officinale polysaccharide (named DOPS‐1) was isolated from the stems of Dendrobium officinale by hot‐water extraction and purified by using Sephadex G‐150 column chromatography. The structural characterization, antioxidant and cytotoxic activity were carried out. Based on the results of HPLC, GC, Congo red experiment, together with periodate oxidation, Smith degradation, SEM, FT‐IR, and NMR spectral analysis, it expressed that DOPS‐1 was largely composed of mannose, glucose and galacturonic acid in a molar ratio of 3.2 : 1.3 : 1. The molecular weight of DOPS‐1 was 1530 kDa and the main chain was composed of (1→4)‐β‐D‐Glcp, (1→4)‐β‐D‐Manp and 2‐O‐acetyl‐(1→4)‐β‐D‐Manp. The measurement results of antioxidant activity showed that DOPS‐1 had the strong scavenging activities on hydroxyl radicals, DPPH radicals and superoxide radicals and the high reducing ability in vitro. Moreover, DOPS‐1 was cytotoxic to all three human cancer cells of MDA‐MB‐231, A549 and HepG2.

Isolation and Structural Characterization of Two Polysaccharides from Dracocephalum moldavica and Their Anti‐Complementary Activity

“…[24] The absorption peaks at 902.45 cm À 1 and 812.03 cm À 1 were attributed to the αand β-type glycosidic bond, respectively. [25] In addition, no absorption peak was found at about 1730 cm À 1 , indicating that they do not contain uronic acid, which is consistent with the results of chemical composition. [26]…”

“…The characteristic bands at 1000∼1200 cm −1 were attributed to the asymmetric glycosidic band vibrations of C−O−C, which indicated the existence of pyran rings [24] . The absorption peaks at 902.45 cm −1 and 812.03 cm −1 were attributed to the α ‐ and β ‐type glycosidic bond, respectively [25] . In addition, no absorption peak was found at about 1730 cm −1 , indicating that they do not contain uronic acid, which is consistent with the results of chemical composition [26] …”

Polysaccharides from fungi: A review on their extraction, purification, structural features, and biological activities

“…In addition, previous scientific studies have introduced numerous discoveries, indicating that fungal polysaccharides have various biological activities, such as, immunomodulatory, antioxidant, anti-tumor, hepatoprotective, anti-inflammatory, anti-aging, hypolipidemic, and radioprotective activities ( Fan et al, 2014 , Hoang et al, 2015 , Wang et al, 2021a , Yang et al, 2006 , Zhang et al, 2021 ). Moreover, the extraction and purification methods, structural characteristics, and chemical modification of fungal polysaccharides have been widely studied, and a certain research foundation has been achieved ( Ahmad, 2021 , Jiang et al, 2021 , Wang et al, 2021b ).…”

Research progress on extraction, purification, structure and biological activity of Dendrobium officinale polysaccharides

“…Currently, anion chromatography columns (DEAE-cellulose and DEAE-Sepharose Fast Flow) separated acidic polysaccharides and medium polysaccharides according to polysaccharide polarity ( 37 – 39 ). Gel filtration chromatography (Sephadex-G series and sephacryl-S series) separated polysaccharides based on molecular weight differences ( 40 – 42 ). For instance, Xie et al ( 39 ) used a DEAE-Cellulose column (1.6 cm × 60 cm) to elute with water and 0.05 M NaCl solution to obtain two separated fractions (DOP-W and DOP-S).…”