Purification and structural characterization of glycolipid biosurfactants fromPseudomonas aeruginosa YPJ-80

Park, Oh-Jin; Lee, Young‐Eun; Cho, Joong-Hoon; Shin, Hyun‐Jae; Yoon, Byung-Dae; Yang, Ji‐Won

doi:10.1007/bf02932503

Cited by 11 publications

(1 citation statement)

References 21 publications

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“…From spectra interpretation, it was assumed that biosurfactants produced by the microorganisms would be peptides with long hydrophobic alkyl chains, which agrees with the literature data. Indeed, biosurfactants can be mainly lipidic or peptidic [32][33][34][35], the peptidic biosurfactants usually being identified by typical absorption bands (1640 and 1518 cm −1 reflecting N-H, C=O, and C-N groups identifying amide functions, while 1368, 1450, and 2960 cm −1 reflect aliphatic chains -CH 3 and -CH 2 -) [35]. The formation of a foam preventing the recycling of the PDMS is therefore the main issue to solve in order to implement TPPB at an industrial scale.…”

Section: Separationmentioning

confidence: 99%

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

et al. 2022

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The treatment of a synthetic polluted gas containing seven volatile organic compounds (VOCs) was studied using a pilot plant in real industrial conditions. The process combined VOC absorption in silicone oil (PolyDiMethylSiloxane, i.e., PDMS), a biological regeneration of the PDMS in a two-phase partitioning bioreactor (TPPB), and a phase separation including settling and centrifugation. The TPPB was operated at a water/PDMS volume ratio of 75/25. The VOCs treatment performance was efficient during the entire test, corresponding to 10 PDMS regeneration cycles. The analysis of the content of the aqueous phase and PDMS confirmed that VOCs are progressively degraded until mineralization. The nitrogen consumption and the characterization of the microorganisms highlighted possible anoxic functioning of the biomass within the first decanter. Moreover, although the absorption and biodegradation performances were very satisfactory, the separation of all phases, essential for the PDMS recycling, was problematic due to the production of biosurfactants by the microorganisms, leading to the formation of a stable emulsion and foaming episodes. As a consequence, the packed column showed slight fouling. However, no significant increase in the pressure drop of the packed bed, as well as no significant impact on VOC absorption efficiency was observed.

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Section: Separationmentioning

confidence: 99%

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

et al. 2022

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Production and Characterisation of Glycolipid Biosurfactant by Halomonas sp. MB-30 for Potential Application in Enhanced oil Recovery

Dhasayan

Kiran

Selvin

2014

Appl Biochem Biotechnol

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Biosurfactant-producing Halomonas sp. MB-30 was isolated from a marine sponge Callyspongia diffusa, and its potency in crude oil recovery from sand pack column was investigated. The biosurfactant produced by the strain MB-30 reduced the surface tension to 30 mN m(-1) in both glucose and hydrocarbon-supplemented minimal media. The critical micelle concentration of biosurfactant obtained from glucose-based medium was at 0.25 mg ml(-1) at critical micelle dilution 1:10. The chemical structure of glycolipid biosurfactant was characterised by infrared spectroscopy and proton magnetic resonance spectroscopy. The emulsification activity of MB-30 biosurfactant was tested with different hydrocarbons, and 93.1 % emulsification activity was exhibited with crude oil followed by kerosene (86.6 %). The formed emulsion was stable for up to 1 month. To identify the effectiveness of biosurfactant for enhanced oil recovery in extreme environments, the interactive effect of pH, temperature and salinity on emulsion stability with crude oil and kerosene was evaluated. The stable emulsion was formed at and above pH 7, temperature >80 °C and NaCl concentration up to 10 % in response surface central composite orthogonal design model. The partially purified biosurfactant recovered 62 % of residual crude oil from sand pack column. Thus, the stable emulsifying biosurfactant produced by Halomonas sp. MB-30 could be used for in situ biosurfactant-mediated enhanced oil recovery process and hydrocarbon bioremediation in extreme environments.

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Membrane Filtration of Biosurfactants

Jauregi

Kourmentza

2019

Separation of Functional Molecules in Food by Membrane Technology

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Purification and structural characterization of glycolipid biosurfactants fromPseudomonas aeruginosa YPJ-80

Cited by 11 publications

References 21 publications

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

Removal of a Mixture of Seven Volatile Organic Compounds (VOCs) Using an Industrial Pilot-Scale Process Combining Absorption in Silicone Oil and Biological Regeneration in a Two-Phase Partitioning Bioreactor (TPPB)

Production and Characterisation of Glycolipid Biosurfactant by Halomonas sp. MB-30 for Potential Application in Enhanced oil Recovery

Membrane Filtration of Biosurfactants

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