Purification and structure determination of four bioactive molecules from a newly isolated Streptomyces sp. TN97 strain

Mehdi, Raoudha Ben Ameur; Sioud, Samiha; Fguira, Lilia Fourati Ben; Béjar, Samír; Mellouli, Lotfi

doi:10.1016/j.procbio.2006.02.010

Cited by 34 publications

(9 citation statements)

References 22 publications

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“…The chemical structures of these three compounds are named as irumamycin (1a), X-14952B (1b) and17-hydroxy-venturicidin A (1c), which possess potent antimicrobial activities against gram-positive, gram-negative bacteria and fungi (Fguira et al 2005). Similar studies were carried out by Mehdi et al (2006) in which the strain Streptomyces sp. TN97 produces three compounds, which are diketopiperazine derivatives, isocoumarin derivative and the N -acetyl-tyramine exhibits potent antimicrobial activities against gram-positive, gram-negative bacteria and fungi (Mehdi et al 2006) and also compounds 5,7-dimethoxy-4- p -methoxylphenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin isolated from S. aureofaciens CMUAc130 exhibited antifungal activities against tested fungi, and their MICs were found to be 120 and 150 µg/ml respectively and also two pure active compounds were isolated from Streptomyces sp.…”

Section: Resultssupporting

confidence: 79%

“…Similar studies were carried out by Mehdi et al (2006) in which the strain Streptomyces sp. TN97 produces three compounds, which are diketopiperazine derivatives, isocoumarin derivative and the N -acetyl-tyramine exhibits potent antimicrobial activities against gram-positive, gram-negative bacteria and fungi (Mehdi et al 2006) and also compounds 5,7-dimethoxy-4- p -methoxylphenylcoumarin and 5,7-dimethoxy-4-phenylcoumarin isolated from S. aureofaciens CMUAc130 exhibited antifungal activities against tested fungi, and their MICs were found to be 120 and 150 µg/ml respectively and also two pure active compounds were isolated from Streptomyces sp. US24 possessed antibacterial activity against Gram-positive and Gram-negative bacteria (Mellouli et al 2003).…”

Section: Resultssupporting

confidence: 79%

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Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity

et al. 2017

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The strain Streptomyces coelicoflavus BC 01 was isolated from mangrove soil and used as inoculum for submerged fermentation. The fermented broth was extracted with ethyl acetate, the crude extract was subjected to silica gel column chromatography and the homogeneity of the isolated fractions was determined by TLC and then subjected to RP-HPLC for their purity. The purification steps led to the isolation of three pure bioactive compounds named as BC 01_C1, BC 01_C2 and BC 01_C3. The chemical structure of these three compounds was established on the basis of their spectroscopic studies like UV, IR, 1H and 13C NMR and GC–MS data by comparison with reference data from literature. The structure of the compound BC 01_C1 was established as 5-amino-2-(6-(2-hydroxyethyl)-3-oxononyl) cyclohex-2-enone. The compound BC 01_C2 was established as8-(aminomethyl)-7-hydroxy-1-(1-hydroxy-4-(hydroxylmethoxy)-2,3-dimethylbutyl)-2-methyl dodecahydro phenanthren-9(1H)-one and the compound BC 01_C3 was established as1-((E)-2-ethylhex-1-en-1-yl)2-((E)-2-ethylidenehexyl)cyclohexane-1,2-dicarboxylate. The MIC values of the three isolated compounds (BC 01_C1, BC 01_C2 and BC 01_C3) were found between 12.5–75 μg/ml for bacteria and 50–125 μg/ml for fungi used in this study. These compounds also possess in vitro antioxidant and anti-inflammatory activities.

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Section: Resultssupporting

confidence: 79%

Section: Resultssupporting

confidence: 79%

Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity

et al. 2017

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“…TN97 (Mehdi et al 2006). This compound was reported to strongly inhibit horse radish peroxidase, to show antitumor activity (McGraw and Hemingway 1977), antiviral (Tang et al 2000), antimicrobial properties (Mehdi et al 2006); it had weak nematocidal activity (Fig. 5) in our experiments, although it significantly increase J2 mortality.…”

Section: Discussionmentioning

confidence: 99%

Nematicidal activity of fervenulin isolated from a nematicidal actinomycete, Streptomyces sp. CMU-MH021, on Meloidogyne incognita

Ruanpanun

Laatsch

Tangchitsomkid³

et al. 2010

World J Microbiol Biotechnol

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An isolate of the actinomycete, Streptomyces sp. CMU-MH021 produced secondary metabolites that inhibited egg hatch and increased juvenile mortality of the root-knot nematode Meloidogyne incognita in vitro. 16S rDNA gene sequencing showed that the isolate sequence was 99% identical to Streptomyces roseoverticillatus. The culture filtrates form different culture media were tested for nematocidal activity. The maximal activity against M. incognita was obtained by using modified basal (MB) medium. The nematicidal assay-directed fractionation of the culture broth delivered fervenulin (1) and isocoumarin (2). Fervenulin, a low molecular weight compound, shows a broad range of biological activities. However, nematicidal activity of fervenulin was not previously reported. The nematicidal activity of fervenulin (1) was assessed using the broth microdilution technique. The lowest minimum inhibitory concentrations (MICs) of the compound against egg hatch of M. incognita was 30 μg/ml and juvenile mortality of M. incognita increasing was observed at 120 μg/ml. Moreover, at the concentration of 250 μg/ml fervenulin (1) showed killing effect on second-stage nematode juveniles of M. incognita up to 100% after incubation for 96 h. Isocoumarin (2), another bioactive compound produced by Streptomyces sp. CMU-MH021, showed weak nematicidal activity with M. incognita.

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“…The amplification of the 16S rRNA gene was performed by PCR using the High Fidelity PCR Master Kit (Roche) and two primers P1 and P2 [13], P1. Denaturation of the target DNA at 94°C for 5 min followed by 30 cycles at 94°C for 1 min, primer annealing at 55°C for 1 min, and primer extension at 68°C for 1 min.…”

Section: Dna Extraction Pcr Amplification and Sequence Analysismentioning

confidence: 99%

Antifungal Molecules Produced by a New Salt-Tolerant and Alkaliphilic Streptomyces sp. BS30 Isolated from an Arid Soil

Souagui

Grosdemange-Billiard

Tritsch

et al. 2015

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci.

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A salt-tolerant and alkaliphilic actinomycete BS30 isolated from a Saharan soil sample of Algeria, exhibited optimum growth at 2 % (w/v) NaCl and pH10. The strain exhibited antimicrobial activity against yeast and toxinogenic fungi. Analysis of the 16S rRNA gene sequence (1476 nucleotides) of the BS30 strain showed 98.77 % similarity with 16S rRNA gene sequence of Streptomyces spiroverticillatus NBRC 12821 T , the most closely related type species. The phylogenetic analysis indicated that the BS30 strain represent a distinct phyletic line suggesting a potential new genomic species. Two bioactive molecules were isolated from the culture broth using various separation and purification steps. The first partially purified bioactive product was subjected to gas chromatography-mass spectrometry (GC-MS). The second fraction was analyzed by ultraviolet-visible (UV-VIS) spectrometry, electro-spray ionization mass (ESI-MS) and high resolution mass spectrometry (HR-ESI-MS) coupled with analytical liquid chromatography (HPLC) with a diode array detector (DAD). The two compounds showed significant antifungal activity against Aspergillus niger 2CA936.

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Purification and structure determination of four bioactive molecules from a newly isolated Streptomyces sp. TN97 strain

Cited by 34 publications

References 22 publications

Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity

Purification and structural elucidation of three bioactive compounds isolated from Streptomyces coelicoflavus BC 01 and their biological activity

Nematicidal activity of fervenulin isolated from a nematicidal actinomycete, Streptomyces sp. CMU-MH021, on Meloidogyne incognita

Antifungal Molecules Produced by a New Salt-Tolerant and Alkaliphilic Streptomyces sp. BS30 Isolated from an Arid Soil

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