Purification and unique properties in UV and CD spectra of Cd-binding peptide 1 from Schizosaccharomycespombe

Plant metallothioneins

“…These polypeptides, and other y-glutamyl isopeptides in which Gly is either absent or substituted with 8i-alanine, are designated class III metallothioneins (Kojima, 1991). These compounds were first identified and characterized in the fission yeast Schizosaccharomycespombe and termed cadystins (Murasugi et al, 1981;Kondo et al, 1984). Similar polypeptides were subsequently purified from plant cell cultures and termed phytochelatins .…”

Cadmium-Sensitive, cad1 Mutants of Arabidopsis thaliana Are Phytochelatin Deficient

“…These have been named PCs (Grill et al, 1985), cadystins (Kondo et al, 1983), poly-y(EC),G peptides (Tackson et al, 1987), and Cd-binding peptides (Reese and Wagner, 1987a) in various studies and will be referred to here as PCs. PCs appear to be ubiquitous in higher plants (Gekeler et al, 1989) and have also been found in alga1 species (Gekeler et al, 1988) and in some yeasts, particularly Schizosaccharomyces pombe, in which they were first discovered (Murasugi et al, 1981. The structure of PCs suggested that GSH (y-glutamylcysteinylglycine) is the substrate for PC biosynthesis.…”

Brassica juncea Produces a Phytochelatin-Cadmium-Sulfide Complex

“…These metal-binding peptides have been called PC,2 cadystins, Cd-peptides, y(EC),-G, and y-glutamyl peptides; they will be referred to here, as in recent reviews (24,28,30), as PCs. PCs were first discovered in the fission yeast as components of metal-containing complexes from Cd-induced cells (19,20); it was demonstrated that previous reports of metallothionein-like proteins from plants could be attributed to PCs (9) and that PCs were produced by all higher plants tested (7). PCs have also been shown to exist in the algae Chlorella fusca (6) and the yeast Candida glabrata, which produces both PCs and a metallothionein (17,18).…”