Purification by expanded bed adsorption and characterization of an α-amylases FORILASE NTL® from A. niger

Toledo, Andrea; Severo, João Baptista; Souza, Roberto Rodrigues de; Campos, Eleonora; Santana, José Carlos Curvelo; Tambourgi, Elias Basile

doi:10.1016/j.jchromb.2006.08.011

Cited by 28 publications

(33 citation statements)

References 17 publications

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“…This shows that bromelain purification is somehow proportional to the expansion degree or flow velocities under the conditions studied here. Similar results were found by Toledo et al (2006) working with α-amylase. It also agrees with the RTD study, because the bed height increases the contact between enzymes and the ion-exchange resin, Amberlite IRA 410.…”

Section: Expanded Bed Adsorption Of Bromelainsupporting

confidence: 87%

“…Analyzing the RTD obtained after a perfect iontracer pulse (axial dispersion, plug-flow exchange of mass with stagnant zones) gave a quantitative description of the underlying hydrodynamic situation during the EBA process (Fernandez-Lahore et al, 2001). Table 1 shows the RTD results after substitution of data into equations (1) to (3), according to the methodology used by several authors (Santos et al, 2000;Fernandez-Lahore et al, 2001;Yamamoto et al, 2001;Toledo et al, 2006).…”

Section: Sds-pagementioning

confidence: 99%

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Expanded bed adsorption of bromelain (E.C. 3.4.22.33) from Ananas comosus crude extract

Silveira

Souza-Jr²,

Santana

et al. 2009

Braz. J. Chem. Eng.

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-This work focuses on the adsorption of Bromelain in expanded bed conditions, such as the adsorption kinetics parameters. The adsorption kinetics parameters showed that after 40 minutes equilibrium was achieved and maximum adsorption capacity was 6.11 U per resin mL. However, the maximum adsorption capacity was only determined by measuring the adsorption isotherm. Only by the Langmuir model the maximum adsorption capacity, Qm, and dissociation constant, kd, values could be estimated as 9.18 U/mL and 0.591, respectively, at 25 °C and 0.1 mol/L phosphate buffer pH 7.5. A column made of glass with an inner diameter of 1 cm was used for the expanded bed adsorption (EBA). The residence time was reduced 10 fold by increasing the expansion degree 2.5 times; nonetheless, the plate number (N) value was reduced only 2 fold. After adsorption, the bromelain was eluted in packed bed mode, with a downward flow. The purification factor was about 13 fold and the total protein was reduced 4 fold. EBA showed to be feasible for purification of bromelain.

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Section: Expanded Bed Adsorption Of Bromelainsupporting

confidence: 87%

Section: Sds-pagementioning

confidence: 99%

Expanded bed adsorption of bromelain (E.C. 3.4.22.33) from Ananas comosus crude extract

Silveira

Souza-Jr²,

Santana

et al. 2009

Braz. J. Chem. Eng.

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“…Na sequência, a eluição das enzimas é feita com o NaCl 0,25M, fazendo com que estas se desprendam das resinas (o que faz aparecerem os pequenos picos), sendo assim purificadas. e guardado a 5 °C (BIAZUS et al, 2005a(BIAZUS et al, , 2005b(BIAZUS et al, , 2006a(BIAZUS et al, , 2006b(BIAZUS et al, , 2006c(BIAZUS et al, , 2007CURVELO-SANTANA, 2003;FERREIRA et al, 2007;SEVERO Jr. et al, 2007;TOLEDO et al, 2007).…”

Section: Adsorção Em Leito Expandidounclassified

“…Então, 5 mL da solução de malte de milho a 2% no tampão usado foram injetados abaixo do leito da coluna para promover a adsorção das enzimas sobre a resina Amberlite IRA 410. A eluição foi feita pelo uso de uma solução de NaCl 0,25 M a 14 mL/minuto de fluxo descendente (BIAZUS et al, 2006a;KALIL, 2000;SANTOS, 2001;TOLEDO et al, 2007). Amostras de 5 mL foram coletadas em tubos de ensaios nas saídas da coluna.…”

Section: • Ensaios Enzimáticosunclassified

“…Rendimento da purificação: a eficiência dos processos foi avaliada por dois parâmetros, dados pelas Equações 1 e 2. A primeira é a recuperação da atividade enzimática (RA) que compara a atividade total no material purificado com a atividade total no material bruto (dado em porcentagem) e a segunda é o fator de purificação (FP) que compara a atividade enzimática específica total no material purificado com a atividade enzimática específica total no material bruto (BIAZUS et al, 2006a(BIAZUS et al, , 2007SANTOS, 2001;TOLEDO et al, 2007), como Equações 1 e 2:…”

Section: Introductionunclassified

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Purificação de amilases de malte de Zea mays

Biazus¹,

Santana²,

Souza³

et al. 2010

Ciênc. Tecnol. Aliment.

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Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

Gautério

Malta

Reginatto

et al. 2017

J of Chemical Tech & Biotech

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BACKGROUND Once peroxidase purification can improve substrate degradation capacity, peroxidase from rice bran was purified by expanded bed adsorption (EBA) coupled to diafiltration/ultrafiltration (DF/UF) and applied for deoxynivalenol (DON) reduction. RESULTS The DF/UF step was optimized according to membrane molecular weight cutoff (MWCO), DF cycles number, operation temperature and CaCl2 addition to the DF buffer. DON reduction tests were carried out with both clarified and purified peroxidase. The use of MWCO of 10 kDa, 10 °C, six DF cycles and adding 4 mmol L−1 CaCl2 to DF buffer promoted enzyme purification of 4.4‐fold and enzymatic recovery of 79.4%. The global purification process combining two techniques resulted in enzyme purification of 75.1‐fold with an enzymatic recovery of 22.8%. Peroxidase purified by EBA combined with DF/UF resulted in DON reduction of 81.7% in a model system. CONCLUSION Peroxidase purification was achieved by scale‐up integrated strategies. The use of purified peroxidase from agricultural by‐products could be a promising alternative for enzymatic DON levels reduction. © 2017 Society of Chemical Industry

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Purification by expanded bed adsorption and characterization of an α-amylases FORILASE NTL® from A. niger

Cited by 28 publications

References 17 publications

Expanded bed adsorption of bromelain (E.C. 3.4.22.33) from Ananas comosus crude extract

Expanded bed adsorption of bromelain (E.C. 3.4.22.33) from Ananas comosus crude extract

Purificação de amilases de malte de Zea mays

Use of partially purified peroxidase of agricultural by‐product rice bran in deoxynivalenol reduction

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