Purification, cDNA Cloning, and Developmental Expression of the Nodule-Specific Uricase from <i>Phaseolus vulgaris</i> L.

Sánchez, Federico; Campos, Francisco; Padilla, Jaime; Bonneville, Jean‐Marc; Enríquez, Consuelo; Caput, Daniel

doi:10.1104/pp.84.4.1143

Cited by 38 publications

(23 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Few of these proteins have been identified: leghemoglobin, uricase II and a nodule-enhanced glutamine synthetase [2,13,18]. In the same experiment a group of abundant transcripts encoding for proteins in the 30 kd range was detected [2].…”

mentioning

confidence: 77%

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules

et al. 1994

Self Cite

View full text Add to dashboard Cite

In Phaseolus vulgaris there is a nodulin family, Npv30, of ca. 30 kDa, as detected in an in vitro translation assay [2]. We isolated a gene (npv30-1) for one of the members of this family. The nucleotide sequence of the promoter of npv30-1 contains nodule-specific motifs common to other late nodulin genes. The promoter was fused to the GUS reporter gene; this chimeric fusion was introduced into Lotus corniculatus via Agrobacterium rhizogenes transformation. GUS activity was only detected in the infected cells of the nodules of transgenic plants. By contrast, the expression of a 35S-GUS construct was restricted to the uninfected cells and the vascular tissue.

show abstract

mentioning

confidence: 77%

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules

et al. 1994

Self Cite

View full text Add to dashboard Cite

show abstract

“…The 34 and 100 kDa bands were next in abundance. The first one corresponds to uricase II and represented 2°70 of the total nodule soluble protein in P. vulgaris [29]. The 100 kDa band could be equivalent to soybean sucrose synthetase based on data recently reported [32].…”

Section: Construction Of a Cdna Library From P Vulgaris Nodule Poly(mentioning

confidence: 99%

Expression of nodule-specific genes in Phaseolus vulgaris L.

Campos¹,

Padilla²,

Vázquez³

et al. 1987

Plant Mol Biol

Self Cite

View full text Add to dashboard Cite

The identification of some nodule-specific host proteins (nodulins) from common bean (Phaseolus vulgaris L.), a tropical ureide-transporting legume, is described. Particularly, the existence and developmental expression of several abundant nodule-specific transcripts of P. vulgaris are shown, including leghemoglobin, nodulespecific uricase and a group that in vitro translates into a cluster of about 30 kDa products. The expression pattern of nodulins in effective (Fix(+)) nodules compared to ineffective (Fix(-)) ones is also presented. The modified expression of main nodulins observed between these nodules indicates that different levels and/or factors associated with their regulation are involved. The intracellular infection by Rhizobium as a decisive step in the induction of some P. vulgaris nodulins is discussed.

show abstract

“…Nothing is known about expression regulation of these uricases. Synthesis of nodulin-35 takes place during the nodule ontogeny in uninfected cells of Glycine max and Phaseolus vulgaris (Bergmann et al 1983, Nguyen et al 1985, Sfinchez et al 1987, Papadopoulou et al 1995, and the enzyme synthesis is regulated by oxygen at the mRNA level in Glycine max callus tissue Jochimsen 1986, Xue et al 1991). However, little is known about the regulation of uricase synthesis in the green alga Chlamydomonas reinhardtii.…”

Section: Introductionmentioning

confidence: 96%

Urate-mediated regulation of urate oxidase inChlamydomonas reinhardtii

et al. 1998

View full text Add to dashboard Cite

Summary.Expression of uricase (urate oxidase) from Chlamydomonas reinhardtii has been investigated by using specific polyclonal antibodies. By Western blot analyses performed under nondenaturing conditions, a 124 kDa protein band corresponding to active uricase was detected in protein extracts from cells cultured with urate or nitrogen-starved cells. This protein band was absent in cells cultured with ammonium. Besides the 124 kDa band, the antibodies also reacted with a massive protein band, with an apparent molecular mass of 500 kDa, that was detected in all nutritional conditions assayed. In vitro, inactive uricase from cells grown with ammonium was activated by incubation in presence of urate. The appearance of uricase activity in vitro coincided with a decrease of the 500 kDa protein and an increase of the 124 kDa band corresponding to the active enzyme. We suggest that a posttranslational regulation of uricase synthesis takes place in C. reinhardtii, and that urate may be responsible for the assembly or maturation of inactive precursors to form the active uricase.

show abstract

Purification, cDNA Cloning, and Developmental Expression of the Nodule-Specific Uricase from Phaseolus vulgaris L.

Cited by 38 publications

References 11 publications

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules

The expression of a chimeric Phaseolus vulgaris nodulin 30-GUS gene is restricted to the rhizobially infected cells in transgenic Lotus corniculatus nodules

Expression of nodule-specific genes in Phaseolus vulgaris L.

Urate-mediated regulation of urate oxidase inChlamydomonas reinhardtii

Contact Info

Product

Resources

About