Purification, characterization and anti-fatigue activity of polysaccharide fractions from okra (Abelmoschus esculentus(L.)Moench)

Abstract

The aim of this study was to investigate the anti-fatigue activity of polysaccharide fractions from Abelmoschus esculentus (L.) Moench (AE) in mice. After crude polysaccharide (CAEP) was extracted from AE and purified by DEAE cellulose-52 column, two polysaccharide fractions (AEP-1 and AEP-2) were obtained. The structural analysis suggested that AEP-1 and AEP-2 were a RG-I polysaccharide and an AG-II polysaccharide, respectively. According to the results of the weight-loaded swimming test, compared with the negative control group, the CAEP, AEP-1 and AEP-2 treatment groups could prolong the swimming time, decrease serum urea nitrogen (SUN) and blood lactic acid (BLA), and increase hepatic glycogen (HG) and muscle glycogen (MG), which indicated that okra polysaccharides have an effective anti-fatigue activity. Furthermore, our study exhibited the anti-fatigue mechanism of okra polysaccharide was correlated with retarding the accumulation of creatine kinase (CK) and lactate dehydrogenase (LDH) in serum, and enhancing succinate dehydrogenase (SDH), adenosine triphosphate (ATP) and adenosine triphosphatase (ATPase) levels. In addition, the anti-fatigue activity of AEP-1 was stronger than that of AEP-2, and significantly better than that of CAEP. Therefore, AEP-1 and AEP-2 may be the main active anti-fatigue functional substances of AE.

Extraction, isolation, characterisation, antioxidant and anti‐fatigue activities of Pleurotus eryngii polysaccharides

“…Meanwhile, the clearance and metabolism of lactic acid in the muscle were increased, the accumulation of lactic acid decreased, and the occurrence of fatigue was delayed or the eliminated by increasing LDH levels. To a certain extent, fatigue was relieved and the muscle tissues were protected by polysaccharides (Gao et al , ).…”

“…A 1 represents the absorbance of the test solution at 510 nm. A 2 represents the absorbance of H 2 O instead of H 2 O 2 at 510 nm, and A 0 represents of H 2 O at 510 nm instead of the test solution (Gao et al , ; Wang et al , ; Gao et al , ).…”

Phytochemical information and pharmacological activities of Okra (Abelmoschus esculentus): A literature‐based review

“…Furthermore, the organ (e.g., kidney, liver, pancreas, and brain) protective (Doreddula et al, ; Sabitha, Ramachandran, et al, ; Tongjaroenbuangam et al, ) and antifatigue (Gao et al, ) capacity of the A. esculentus may be due to its cytoprotective capacity. The nontoxic nature of the herb may also relate its protective power (Doreddula et al, ).…”

“…Moreover, the polysaccharide fractions of A. esculentus at 50, 100, and 200 (p.o.) mg/kg were found to show an antifatigue activity by decreasing in serum urea nitrogen and blood lactic acid, and increasing in hepatic glycogen and muscle glycogen; as well as by retarding the accumulation of creatine kinase and lactate dehydrogenase in serum, and enhancing succinate dehydrogenase, adenosine triphosphate, and adenosine triphosphatase levels in male Kunming mice ( n = 30) (Gao et al, ). Postulated mechanism of action in the literatures of some biological activities has been shown in Table .…”

Anti-Fatigue Effects of Yogurt Fermented with Lactobacillus delbrueckii subsp. bulgaricus OLL1073R-1 in Healthy People Suffering from Summer Heat Fatigue: A Randomized, Double-Blind, Placebo-Controlled Trial

“…Potent anti-fatigue activities of foods, plants, medical herbs, and lactic acid bacteria have been reported previously [ 3 , 4 , 5 , 6 , 7 , 8 ]. Some of the active components were expected to be polysaccharides [ 9 , 10 ], and polysaccharides purified from fungi, marine algae, and green tea were shown to exhibit anti-fatigue effects in animal studies after physiological challenges such as swimming and running [ 11 , 12 , 13 ]. However, few human trials have investigated the effects of food or its components on the chronic fatigue that healthy people suffer in daily life as in the case of summer heat fatigue.…”