Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae

Li, Jinyu; Yuan, Peng; Wang, Xinhui; Aipire, Adila; Li, Minjing; Yang, Jie; Ying, Tianlei; Fu, Changshuang; Wei, Xianxian; Zhang, Fuchun

doi:10.1039/c7fo00227k

Cited by 46 publications

(23 citation statements)

References 30 publications

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“…The monosaccharide composition of PEP was analyzed according to the literature [43]. To analyze the monosaccharide composition, PEP was hydrolyzed with 4 M trifluoroacetic acid at 120 • C for 8 h. The hydrolyzed products of PEPs were derived with PMP according to the previous description [44].…”

Section: Preparation Of Polysaccharidesmentioning

confidence: 99%

Antioxidant, Hypolipidemic and Hepatic Protective Activities of Polysaccharides from Phascolosoma esculenta

Jiang

Lin

et al. 2020

Marine Drugs

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The aims of this study were to investigate the antioxidant, hypolipidemic and hepatic protective effects of Phascolosoma esculenta polysaccharides (PEP). PEP was prepared from Phascolosoma esculenta by enzyme hydrolysis and its characterization was analyzed. The antioxidant activities of PEP were evaluated by the assays of scavenging 1,1-Diphenyl-2-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radicals and chelating ferrous ion in vitro. It showed that PEP could scavenge radicals effectively and had favorable antioxidant activities. In the meantime, the hypolipidemic effect of PEP was investigated in vivo by using mice model fed with high-fat diet with or without PEP treatment. Compared with the hyperlipidemic mice without treatment, the serum levels of total cholesterol (TC) (30.1–35.7%, p < 0.01), triglyceride (TG) (24.5–50.8%, p < 0.01 or p < 0.05), low-density lipoprotein cholesterol (LDL-C) (49.6–56.8%, p < 0.01) and liver levels of TC (21.0–28.4%, p < 0.01), TG (23.8–37.0%, p < 0.01) decreased significantly, whereas serum high-density lipoprotein cholesterol (HDL-C) (47.7–59.9%, p < 0.01 or p < 0.05) increased significantly after treatment with different dosage of PEP (0.2, 0.4 and 0.8 g per kg body weight, respectively). In addition, superoxide dismutase (SOD) (10.2–22.2% and 18.8–26.9%, p < 0.05), glutathione peroxidase (GSH-Px) (11.9–15.4% and 26.6–30.4%, p < 0.05) activities in serum and liver enhanced markedly while aspartate aminotransferase (AST) (18.7–29.6% and 42.4–58.0%, p < 0.05), alanine transaminase (ALT) (42.7–46.0% and 31.2–42.2%, p < 0.05) activities, as well as the levels of malondialdehyde (MDA) (15.9–24.4% and 15.0–16.8%, p < 0.01 or p < 0.05) in serum and liver reduced markedly. Moreover, the histopathological observation of livers indicated that PEP could attenuate liver cell injury. The animal experimental results demonstrated that PEP exerted hypolipidemic and hepatoprotective roles in hyperlipidemic mice. In summary, our results above suggest that PEP might be a potential natural antioxidant and utilized as a therapeutic candidate for hyperlipidemia.

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Section: Preparation Of Polysaccharidesmentioning

confidence: 99%

Antioxidant, Hypolipidemic and Hepatic Protective Activities of Polysaccharides from Phascolosoma esculenta

Jiang

Lin

et al. 2020

Marine Drugs

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“…GUPS-C was further purified by DEAE-52 chromatography according to our previous protocol with a minor modification [11]. In brief, 0.5 g of GUPS-C was dissolved in 200 mL distilled water and filtered through 0.22 µM filter.…”

Section: Purification Of Gupsmentioning

confidence: 99%

“…Many plant-derived polysaccharides have been extensively studied for immune enhancing effect because of their safety profiles [9]. Polysaccharides can activate macrophages, dendritic cells (DCs), T lymphocytes, B lymphocytes, and natural killer cells to produce immune-related molecules, such as cytokines, antibodies, and complement molecules [10][11][12]. Recently, several studies have shown that polysaccharides bind to receptors such as Toll-like receptors (TLRs) on the surface of macrophages or DCs to trigger several down-stream signaling pathways to promote immune responses [13,14].…”

Section: Introductionmentioning

confidence: 99%

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

et al. 2020

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Glycyrrhiza uralensis is a Chinese herbal medicine with various bioactivities. Three fractions (GUPS-I, GUPS-II and GUPS-III) of G. uralensis polysaccharides (GUPS) were obtained with molecular weights of 1.06, 29.1, and 14.9 kDa, respectively. The monosaccharide compositions of GUPS-II and GUPS-III were similar, while that of GUPS-I was distinctively different. The results of scanning electron microscopy, FT-IR, and NMR suggested that GUPS-II and GUPS-III were flaky with a smooth surface and contained α- and β-glycosidic linkages, while GUPS-I was granulated and contained only α-glycosidic linkages. Moreover, GUPS-II and GUPS-III exhibited better bioactivities on the maturation and cytokine production of dendritic cells (DCs) in vitro than that of GUPS-I. An in vivo experiment showed that only GUPS-II significantly enhanced the maturation of DCs. These results indicate that GUPS-II has the potential to be used in combination with cancer immunotherapy to enhance the therapeutic effect.

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“…The monosaccharide composition was determined by HPLC according to our previous report with some modification (J. Li et al, 2017). Briefly, TAPS and OSPS were hydrolysed with 4 M trifluoroacetic acid at 120°C for 2 h, and the fractions were incubated with 1phenyl-3-methyl-5-pyrazolone (PMP, ALADDIN, Shanghai) at 70°C for 60 min.…”

Section: Determination Of Monosaccharide Compositionmentioning

confidence: 99%

“…However, the activity of PFPS might be correlated with its triple-helix conformation (J. Li et al, 2017), which conferred higher immunostimulating activity (Falch et al, 2000).…”

Section: The Immunoregulatory Effects Of These Four Polysaccharides Omentioning

confidence: 99%

Comparison of structural characteristics and immunoregulatory activities of polysaccharides from four natural plants

Mahabati

Aipire

Yuan

et al. 2020

Food and Agricultural Immunology

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Polysaccharides have various bioactivities including anti-tumour, antioxidant and immunoregulation, which are correlated with their structural characteristics. Here, four polysaccharides from Glycyrrhiza uralensis (GUPS), Pleurotus ferulae (PFPS), Triticum aestivum (TAPS) and Oryza sativa (OSPS) were purified to compare their structural characteristics and immunoregulatory activities. The data showed that TAPS, OSPS, GUPS and PFPS contained different monosaccharide components and had distinctly structural characteristics. All the four polysaccharides promoted the maturation of dendritic cells (DCs) through TLR4 with different activities. GUPS and PFPS also reduced the phagocytosis of DCs but not for TAPS and OSPS. Importantly, GUPS and PFPS enhanced the Th1 skewing immune response in mice without inflammation.TAPS and OSPS only showed weak immunostimulatory activity in vivo. The immunostimulatory activities of the four polysaccharides were ranked as PFPS = GUPS > TAPS > OSPS. ARTICLE HISTORY

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Purification, characterization and bioactivities of polysaccharides from Pleurotus ferulae

Cited by 46 publications

References 30 publications

Antioxidant, Hypolipidemic and Hepatic Protective Activities of Polysaccharides from Phascolosoma esculenta

Antioxidant, Hypolipidemic and Hepatic Protective Activities of Polysaccharides from Phascolosoma esculenta

Preparation, Characterization, and Immuno-Enhancing Activity of Polysaccharides from Glycyrrhiza uralensis

Comparison of structural characteristics and immunoregulatory activities of polysaccharides from four natural plants

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