Purification, Characterization, and Biosynthesis of Bovine Cartilage Lysozyme Isoforms

Moss, Jonathan M.; Damme, Marie-Paule Isabelle Van; Murphy, William H.; Stanton, Peter G.; Thomas, Pat; Preston, B. N.

doi:10.1006/abbi.1996.9828

Cited by 16 publications

(6 citation statements)

References 36 publications

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“…Chitosan is a polycationic polysaccharide comprising glucosamine and N-acetylglucosamine residues. Moreover, chitosan can be enzymatically degraded in vivo by lysozyme [13,14], a polycationic protein present in the ECM of human cartilage [15,16]. These features make chitosan a potential material for use in cartilage tissue engineering to modulate chondrocyte morphology, differentiation and stimulating chondrogenesis.…”

Section: Introductionmentioning

confidence: 99%

Injectable chitosan-based hydrogels for cartilage tissue engineering

et al. 2009

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Section: Introductionmentioning

confidence: 99%

Injectable chitosan-based hydrogels for cartilage tissue engineering

et al. 2009

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“…Chitosan administered by intra-articular injection promotes thicker epiphyseal cartilage in the tibial and femoral joints, with the proliferation of chondrocytes. These characteristics make this polymer a potential material for use in cartilage tissue engineering to modulate chondrocyte morphology, modulate differentiation, and stimulate chondrogenesis [96,[131][132][133][134][135][136][137].…”

Section: Cartilage Tissue Engineeringmentioning

confidence: 99%

A Review on Chitosan’s Uses as Biomaterial: Tissue Engineering, Drug Delivery Systems and Cancer Treatment

et al. 2020

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Chitosan, derived from chitin, is a biopolymer consisting of arbitrarily distributed β-(1-4)-linked D-glucosamine and N-acetyl-D-glucosamine that exhibits outstanding properties— biocompatibility, biodegradability, non-toxicity, antibacterial activity, the capacity to form films, and chelating of metal ions. Most of these peculiar properties are attributed to the presence of free protonable amino groups along the chitosan backbone, which also gives it solubility in acidic conditions. Moreover, this biopolymer can also be physically modified, thereby presenting a variety of forms to be developed. Consequently, this polysaccharide is used in various fields, such as tissue engineering, drug delivery systems, and cancer treatment. In this sense, this review aims to gather the state-of-the-art concerning this polysaccharide when used as a biomaterial, providing information about its characteristics, chemical modifications, and applications. We present the most relevant and new information about this polysaccharide-based biomaterial’s applications in distinct fields and also the ability of chitosan and its various derivatives to selectively permeate through the cancer cell membranes and exhibit anticancer activity, and the possibility of adding several therapeutic metal ions as a strategy to improve the therapeutic potential of this polymer.

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“…The abundance of chondrocalcin in cartilage is often used as an indication of new collagen synthesis. Finally, lysozyme [44] and phospholipase A2 [45] are worthy of mention. Both are cationic proteins that may owe their presence in the cartilage matrix to the high content of anionic aggrecan.…”

Section: Proteins Of the Cartilage Extracellular Matrixmentioning

confidence: 99%

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Roughley

2001

Arthritis Res

135

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CILP = cartilage intermediate-layer protein; CMP = cartilage matrix protein; COMP = cartilage oligomeric matrix protein; CS = chondroitin sulfate; CS1/CS2 = chondroitin-sulfate-attachment regions of aggrecan; G1/G2/G3 = globular regions [of aggrecan]; IL-1 = interleukin-1; PRELP = proline-and arginine-rich end leucine-rich repeat protein; SLRP = small leucine-rich repeat proteoglycan.Available online http://arthritis-research.com/content/3/6/342 IntroductionThe extracellular matrix of articular cartilage contains a large variety of noncollagenous proteins. Many of these are listed in Table 1, and while this list is by no means exhaustive, it does include those that have been studied in the most detail. It is impossible to give any common feature that unites this group of molecules, as they vary greatly in structure and function, and in some cases it is not clear that they are even made by the chondrocytes. Many of the molecules are proteoglycans, bearing glycosaminoglycan chains, whereas others are glycoproteins or even nonglycosylated proteins. Some of the molecules represent degradation products of larger precursors that accumulate because of their interaction with other matrix components. Many of the molecules play a structural role, whereas others may be involved in regulating cell function. In addition, many of the molecules vary in their abundance and structure with anatomical site or the person's age, and many are not unique to cartilage. The importance of many of the molecules to cartilage function is illustrated in Table 2, which shows their association with pathology when they are produced in a mutant form. Proteoglycans of the cartilage extracellular matrix Aggregating proteoglycansAmong the noncollagenous proteins of cartilage, aggrecan has undoubtedly received the greatest attention, because of its high abundance in cartilage, its close association with the ability of the tissue to resist compression, and its modification in many cartilage disorders. Aggrecan belongs to the family of aggregating proteoglycans that form large, multimolecular complexes with hyaluronan AbstractCartilage contains numerous noncollagenous proteins in its extracellular matrix, including proteoglycans. At least 40 such molecules have been identified, differing greatly in structure, distribution, and function. Some are present in only selected cartilages or cartilage zones, some vary in their presence with a person's development and age, and others are more universal in their expression. Some may not even be made by the chondrocytes, but may arise by absorption from the synovial fluid. In many cases, the molecules' function is unclear, but the importance of others is illustrated by their involvement in genetic disorders. This review provides a selective survey of these molecules and discusses their structure, function, and involvement in inherited and arthritic disorders.

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Purification, Characterization, and Biosynthesis of Bovine Cartilage Lysozyme Isoforms

Cited by 16 publications

References 36 publications

Injectable chitosan-based hydrogels for cartilage tissue engineering

Injectable chitosan-based hydrogels for cartilage tissue engineering

A Review on Chitosan’s Uses as Biomaterial: Tissue Engineering, Drug Delivery Systems and Cancer Treatment

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