Purification, Characterization, and Gene Expression of All Sigma Factors of RNA Polymerase in a Cyanobacterium

Imamura, Sousuke; Yoshihara, Satoshi; Nakano, Serina; Shiozaki, Noriko; Yamada, Akiko; Tanaka, Kan; Takahashi, Hideo; Asayama, Munehiko; Shirai, Makoto

doi:10.1016/s0022-2836(02)01242-1

Cited by 118 publications

(272 citation statements)

References 62 publications

Supporting

Mentioning

256

Contrasting

Order By: Relevance

“…Rapid transient increase of SigB transcripts has been detected in high-salt stress (Fig. 5), in heat stress (Imamura et al, 2003;Shoumskaya et al, 2005;Tuominen et al, 2006), and after a dark-tolight shift (Tuominen et al, 2003). Furthermore, upregulation of the sigB gene has been found to occur under oxidative stress induced by hydrogen peroxide treatment (Kanesaki et al, 2007) and under nitrogen starvation (Imamura et al, 2006).…”

Section: Discussionmentioning

confidence: 89%

“…It is well documented that the SigB factor is involved in the up-regulation of heat shock genes, especially the hspA gene, at high temperatures (Imamura et al, 2003;Singh et al, 2006;Tuominen et al, 2006). Many heat shock proteins, particularly HspA, are important chaperones .…”

Section: Discussionmentioning

confidence: 99%

“…DNA microarray analysis has shown that the expression of the sigD gene increases in response to high light in Synechocystis (Hihara et al, 2001;Huang et al, 2002). Furthermore, Imamura et al (2003) reported an increase in the amount of SigD protein after high-light treatment. In another cyanobacterium, Synechococcus elongatus PCC 7942, one of the five group 2 s factors, RpoD3, was recognized as a high-light-responsive s factor (Seki et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

“…The sigA gene encodes the principal (group 1) s factor that is essential for cell viability. The sigB, sigC, sigD, and sigE genes encode group 2 s factors (primary-like s factors) that show extensive amino acid similarity with the SigA factor but are not essential for cell viability under optimal growth conditions (Imamura et al, 2003;Tuominen et al, 2003). Recent results have shown that group 2 s factors are important under suboptimal conditions (Osanai et al, 2005;Singh et al, 2006;Tuominen et al, 2006Tuominen et al, , 2008Summerfield and Sherman 2007).…”

mentioning

confidence: 99%

See 3 more Smart Citations

Characterization of Single and Double Inactivation Strains Reveals New Physiological Roles for Group 2 σ Factors in the Cyanobacterium Synechocystis sp. PCC 6803

Pollari

Gunnelius²,

Tuominen³

et al. 2008

Plant Physiology

View full text Add to dashboard Cite

Cyanobacteria are eubacteria that perform oxygenic photosynthesis like plants. The initiation of transcription, mediated by the RNA polymerase holoenzyme, is the main determinant of gene regulation in eubacteria. The s factor of the RNA polymerase holoenzyme is responsible for the recognition of a promoter sequence. In the cyanobacterium Synechocystis sp. PCC 6803, the primary s factor, SigA, is essential for cell viability. The SigB, SigC, SigD, and SigE factors show significant sequence similarity with the SigA factor but are nonessential. In this study, we have used homology modeling to construct a three-dimensional model of Synechocystis RNA polymerase holoenzyme and all group 1 and 2 s factors. According to the models, the overall three-dimensional structures of group 1 and 2 s factors are similar, the SigB and SigD factors being the most similar ones. In addition, we have constructed a complete set of group 2 s factor double inactivation strains, DsigBC, DsigBD, DsigBE, DsigCD, DsigCE, and DsigDE. All double mutants grow well under standard conditions, but differences are observed in stress conditions. The transition from lag phase to exponential growth is slow in the DsigBD strain, and all strains lacking the SigD factor were found to be sensitive to bright light. Furthermore, all group 2 s factors were found to be involved in acclimation to salt-or sorbitol-induced osmotic stresses.

show abstract

Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Characterization of Single and Double Inactivation Strains Reveals New Physiological Roles for Group 2 σ Factors in the Cyanobacterium Synechocystis sp. PCC 6803

Pollari

Gunnelius²,

Tuominen³

et al. 2008

Plant Physiology

View full text Add to dashboard Cite

show abstract

“…The sigG (slr1545) gene is apparently essential for cell viability in this cyanobacterium (Huckauf et al, 2000). Although transcripts of these group 3 sigma factor genes are detectable, the amounts of the proteins in cells are less than the detection limits by immunoblot analysis (Imamura et al, 2003b).…”

mentioning

confidence: 99%

Group 3 sigma factors in the marine cyanobacterium Synechococcus sp. strain PCC 7002 are required for growth at low temperature

Inoue-Sakamoto

Gruber

Christensen

et al. 2007

J. Gen. Appl. Microbiol.

View full text Add to dashboard Cite

Transcription of Plastid Genes

Liere¹,

Börner²

Regulation of Transcription in Plants

View full text Add to dashboard Cite

Purification, Characterization, and Gene Expression of All Sigma Factors of RNA Polymerase in a Cyanobacterium

Cited by 118 publications

References 62 publications

Characterization of Single and Double Inactivation Strains Reveals New Physiological Roles for Group 2 σ Factors in the Cyanobacterium Synechocystis sp. PCC 6803

Characterization of Single and Double Inactivation Strains Reveals New Physiological Roles for Group 2 σ Factors in the Cyanobacterium Synechocystis sp. PCC 6803

Group 3 sigma factors in the marine cyanobacterium Synechococcus sp. strain PCC 7002 are required for growth at low temperature

Transcription of Plastid Genes

Contact Info

Product

Resources

About