Purification, characterization and immobilization of a keratinase from Aspergillus oryzae

Farag, Aïda M.; Hassan, Mohamed A.

doi:10.1016/j.enzmictec.2003.09.002

Cited by 169 publications

(100 citation statements)

References 27 publications

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“…Further, a group of enzymes can be secreted simultaneously to target multiple nutrients (Chaturvedi et al, 2013;Anand et al, 1990). Keratinase is produced by various bacteria, actinomycetes and fungi in an optimum range of physical parameters (Farag and Hassan, 2004;Thys et al, 2004;Anbu et al, 2005). In the presented study, our study reports similar to Sousa et al (2015;Jaroslava et al, (2014).…”

Section: Discussionsupporting

confidence: 81%

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Sharma¹,

Sharma²,

Seth³

2017

American Journal of Applied Sciences

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Earth has innate background for fungi that cover individual kingdom since evolution. The keratinophilic fungi are allied moulds that produce the keratinase enzyme to degrade the keratinous materials in or on the soil. Keratinous materials are insoluble and resistant to degradation by common proteinase enzymes. It is important to study the microorganism producers of such enzymes for use in the biotechnology industry. In order to present study, two isolates of fungi were evaluated to determine if they had the ability to degrade keratin as nutrient substrate. They were grown in an inundated culture medium containing poultry feathers. Among species, best keratin substrate degradation activity as well as keratinase enzyme activity was recorded in Arthoderma multifidium (KU578107) followed by Chrysosporium tropicum (KU578108) gradually leading manner day by days.

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Section: Discussionsupporting

confidence: 81%

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Sharma¹,

Sharma²,

Seth³

2017

American Journal of Applied Sciences

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“…(27%), non-sporulating fungus (17%), and bacteria (10%). As described by Onifade et al [22] and Farag and Hassan [12], most of the keratinolytic fungi are belonging to fungi imperfecti. The identification and characterization of new fungal species able to degrade keratinous waste may help to understand its role in nature.…”

Section: Discussionmentioning

confidence: 99%

“…Most of the keratinases reported to date have been found to be serine proteases [6][7][8][9][10], and a few metalloproteases have shown keratinolytic activity [11,12].…”

Section: Introductionmentioning

confidence: 99%

Purification and properties of a novel fungal alkaline keratinase from Cunninghamella echinulata

More¹,

Divyalakshmi²,

Prakash³

et al. 2013

Turk J Bioch

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“…These temperature profiles are similar to those found by other authors. The enzyme described by Ionata et al (2008) had optimal activity at 55°C, while Farag and Hassan (2004) also reported the highest activities for a keratinase from Aspergillus oryzae at 55°C.…”

Section: Effects Of Ph and Temperature On The Proteolytic Activitiesmentioning

confidence: 99%

Hide unhairing and characterization of commercial enzymes used in leather manufacture

Dettmer

Ayub

Gutterres

2011

Braz. J. Chem. Eng.

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-The enzymatic treatment of hides in tannery processes is a promising technology. However, the reaction kinetics of commercial enzymes available to the leather industry are not fully understood and their activities have been mainly determined with model proteins such as casein as substrate, which are not of direct relevance for cattle hides. Therefore, it is important to determine their activities on collagen and keratin, the main proteins of skin, in order to use these enzymes in leather processing. This work describes the study of five proteases, used commercially in tanneries, to assess their ability to act upon collagen and keratin and to determine their unhairing. Results showed that all commercial enzymes tested had more activity on collagen than on keratin. Unhairing was also tested and four out of the five enzymes tested showed some unhairing activity. Optima of the temperature and pH of the enzymes were very similar for all five enzymes, with maximal activities around 55°C and pH 9 to 12, respectively.

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Purification, characterization and immobilization of a keratinase from Aspergillus oryzae

Cited by 169 publications

References 27 publications

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Evaluation of Keratinolytic Activity Succeeds by Keratinophilic Fungi in Jaipur, India

Purification and properties of a novel fungal alkaline keratinase from Cunninghamella echinulata

Hide unhairing and characterization of commercial enzymes used in leather manufacture

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