Purification, characterization, and molecular cloning of gonadotropin subunits of silver carp (Hypophthalmichthys molitrix)

Chang, Ya-Wen; Huang, Chang‐Jen; Huang, Fore‐Lien; Liu, C.S.; Lo, Tung‐Bin

doi:10.1016/0016-6480(90)90043-l

Cited by 32 publications

(7 citation statements)

References 32 publications

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“…The nucleotide substitution rates were estimated by comparing the GTH-Iß and -Ilß cDNAs of masu The amino acid sequences of the masu salmon gonadotrophin (GTH) ß subunits were compared with those of the European eel (Querat et al 1990), common carp (Chang et al 1988), silver carp (Chang et al 1990), chum salmon (Sekine et al 1989) and chinook salmon (Trinh et al 1986) ß subunits. The positive numbers denote the amino acid residues of the mature masu salmon Iß subunit.…”

Section: Nucleotide Substitution Ratesmentioning

confidence: 99%

Molecular cloning of cDNAs encoding two gonadotrophin β subunits (GTH-Iβ and -IIβ) from the masu salmon, Oncorhynchus masou: rapid divergence of the GTH-Iβ gene

Kato

Kobashi²,

Maruyama³

et al. 1993

Journal of Molecular Endocrinology

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Two types of cDNA (GTH-I beta and -II beta) encoding the beta subunit of masu salmon (Oncorhynchus masou) gonadotrophin were cloned using the reverse transcription-polymerase chain reaction for pituitary mRNAs. The nucleotide sequence of GTH-I beta cDNA was 469 bp long, encoding 137 amino acids, and GTH-II beta cDNA was 476 bp long, encoding 142 amino acids. These two masu salmon beta subunit types showed low homologies of 52% (nucleotide sequence) and 33% (amino acid sequence). The evolutionary interval between masu and chum salmon was estimated to be 5.65 and 1.43 million years by comparing GTH-I beta and GTH-II beta respectively. These time values are markedly inconsistent with the evolutionary time (3.0 million years) estimated from fossil records and an isozyme study. Southern blot analyses showed that the I beta gene restriction fragment lengths differed among five teleosts, whereas, with one exception, the II beta gene showed well conserved patterns. Therefore, the GTH-I beta gene may have diverged at a faster rate than the GTH-II beta gene.

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Section: Nucleotide Substitution Ratesmentioning

confidence: 99%

Molecular cloning of cDNAs encoding two gonadotrophin β subunits (GTH-Iβ and -IIβ) from the masu salmon, Oncorhynchus masou: rapid divergence of the GTH-Iβ gene

Kato

Kobashi²,

Maruyama³

et al. 1993

Journal of Molecular Endocrinology

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“…FSH and LH are complex heterodimer glycoproteins, composed of a common a-subunit and a hormone specific beta subunit (Li and Ford 1998). Since two chemically distinct GTHs were identified in chum salmon (Oncorhynchus keta) (Suzuki et al 1988a; Kawauchi et al 1989), the properties, structural and expression analyses of FSHb, LHb and common a-subunit have been reported in a number of teleost species (Banerjee and Khan 2007;Chang et al 1988Chang et al ,1990Elizur et al 1996;Hassin et al 1995;Jackson et al 1999;Jeng et al 2007;Kagawa et al1998;Kajimura et al 2000;Kim et al 2007;Li et al 2005;So et al 2005;Van Der Kraak et al 1992, Yoshiura et al 1997.…”

Section: Introductionmentioning

confidence: 98%

Molecular cloning, characterization and expression of FSH and LH beta subunits from grass carp (Ctenopharyngodon idella)

Zhou

Niu

Tao

et al. 2008

Fish Physiol Biochem

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Follicle-stimulating hormone beta subunit (FSHbeta) and luteinizing hormone beta subunit (LHbeta) have been cloned and characterized from the pituitary of grass carp (Ctenopharyngodon idella). The full length of FSHbeta and LHbeta cDNA was 393 bp and 441 bp, with open reading frame encoding proteins of 130 and 146 amino acids, respectively. Phylogenetic analysis of the proteins of FSHbeta and LHbeta showed a high homology with other fishes. Homology analysis also indicated that LHbeta has higher conservation than FSHbeta. The expression analysis of grass carp FSHbeta and LHbeta by RT-PCR suggested that they were only expressed in the pituitary. Real-time quantitative PCR protocols were developed and validated to measure FSHbeta and LHbeta mRNAs during ovarian development. The FSHbeta and LHbeta mRNA level was very low in the pituitaries of early-pubertal fish and significantly increased during the ovulation period. These results suggested that in grass carp the gonadotropins synthesized synchronously in order for asynchronous oogenesis to take place.

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“…It is now firmly established that in several salmonid species, in carp and in tuna two chemically distinct GTHs, GTH-I and GTH-II, are synthetized in the pituitary (Kawauchi et al 1986;Ando and Ishii 1988;Suzuki et al 1988 a,b;Chang et al 1990). The question is raised whether these two hormones are synthetized and secreted by two gonadotropic cell types.…”

Section: Introductionmentioning

confidence: 98%

GTH-cells in the pituitary of the African catfish, Clarias gariepinus, during gonadal maturation: an immuno-electron microscopical study

Schulz

et al. 1993

Fish Physiol Biochem

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In an ultrastructural immunocytochemical study we investigated the development of the gonadotropic cells in the pituitary of two to six months old male African catfish in relation to testicular development. In this period, pituitary and testicular tissue samples were collected on five occasions (groups I-V). Blood samples could only be taken from the fish in groups III-V. The testicular development was divided in three stages i.e., immature (only spermatogonia, group I), early (spermatogonia and spermatocytes, groups II and III) and advanced (all germ cell stages including spermatozoa, groups IV and V) spermatogenesis. 11-Ketotestosterone blood levels were low, except for the last group. Antisera were raised against the complete catfish α,βGTH-II, as well as to the separate α- and β-subunits of catfish GTH-II. In the proximal pars distalis of immature fish, undifferentiated cells, somatotrops, putative thyrotrops (pTSH) and putative gonadotrops (pGTH) were found. In the two latter, secretory granules were labeled with anti-αGTH, but not with anti-βGTH-II. pTSH- and pGTH-cells were distinguished on the basis of the size of their secretory granules. During early spermatogenesis, two classes of putative gonadotrops could be distinguished. One type had the same immunocytochemical and ultrastructural characteristics as in immature fish; the secretory granules in the second cell type, which was more abundant, were also immunopositive for anti-βGTH-II. The mean volume of the secretory granules in these GTH-II cells was three times larger than that in the early appearing pGTH-cells. In addition, the later appearing GTH-II cells contained large inclusions, known as globules. These structures labeled with anti-αβGTH-II and with anti-βGTH-II, but not with anti-αGTH. It is assumed that the globules are involved in a differential storage and/or breakdown of the GTH-II subunits. During advanced spermatogenesis the two gonadotropic cell types could still be distinguished, but the early appearing pGTH-cell type was scarce. The present observations permit the conclusion that the early appearing cells may be GTH-I cells. However, definitive proof about their identity depends on the availability of antibodies or cDNA probes specific for GTH-I.

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Purification, characterization, and molecular cloning of gonadotropin subunits of silver carp (Hypophthalmichthys molitrix)

Cited by 32 publications

References 32 publications

Molecular cloning of cDNAs encoding two gonadotrophin β subunits (GTH-Iβ and -IIβ) from the masu salmon, Oncorhynchus masou: rapid divergence of the GTH-Iβ gene

Molecular cloning of cDNAs encoding two gonadotrophin β subunits (GTH-Iβ and -IIβ) from the masu salmon, Oncorhynchus masou: rapid divergence of the GTH-Iβ gene

Molecular cloning, characterization and expression of FSH and LH beta subunits from grass carp (Ctenopharyngodon idella)

GTH-cells in the pituitary of the African catfish, Clarias gariepinus, during gonadal maturation: an immuno-electron microscopical study

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