Purification, identification, and characterization of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from alcalase digested horse gram flour

Bhaskar, Bincy; Ananthanarayan, Laxmi; Jamdar, Sahayog N.

doi:10.1016/j.lwt.2018.12.059

Cited by 57 publications

(41 citation statements)

References 29 publications

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“…These structural features all significantly contributed to its relatively high ACE-inhibitory activity (72.24 µM), which was higher (p < 0.05) than that of the peptides identified from horrens protein (GSAGY, IC 50 : 706 µM) and casein (NMAINPSKENLCSTFCK, IC 50 : 129.07 µM) [38,40]. Furthermore, the Arg at the N-terminal of RWDISQPY contributed substantially to the high activity [41]. An increasing number of studies have found that, apart from molecular mass and C-terminal tripeptides, other amino acid sequences such as Nutrients 2020, 12, 653 9 of 14 the N-terminal residue, C-terminal tetrapeptide, pentapeptide, and even heptapeptide of peptides also contribute to the inhibitory activity [25,41].…”

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 95%

“…Compared to noncompetitive and uncompetitive inhibitors, competitive peptides seem to exhibit relatively higher ACE-inhibitory activity [4]. Bhaskar et al [41] and Lin et al [28] also identified peptides TVGMTAKF, QLLLQQ and PFPGPIPN with competitive inhibition patterns. Now, it has been demonstrated that hydrogen bonds formed between the ACE active sites (S1, S1 , and S2) and peptides' C-terminal tripeptide are crucial to ACE inhibition [6].…”

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

“…Furthermore, the Arg at the N-terminal of RWDISQPY contributed substantially to the high activity [41]. An increasing number of studies have found that, apart from molecular mass and C-terminal tripeptides, other amino acid sequences such as Nutrients 2020, 12, 653 9 of 14 the N-terminal residue, C-terminal tetrapeptide, pentapeptide, and even heptapeptide of peptides also contribute to the inhibitory activity [25,41].…”

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

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Efficacy of a Novel ACE-Inhibitory Peptide from Sargassum maclurei in Hypertension and Reduction of Intracellular Endothelin-1

2020

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Sargassum maclurei is a potential protein resource because of its high protein content and relatively balanced amino acid composition. To promote its usage in food, medical, or other industries, S. maclurei protein was hydrolyzed by pepsin and papain to obtain bioactive peptides. The S. maclurei protein hydrolysates (SMPHs) were purified using gel chromatography and reversed-phase high performance liquid chromatography (RP-HPLC), and 12 major fractions were obtained. The fraction D11 with the highest angiotensin I-converting enzyme (ACE) inhibition (61.59%, at 1 mg/mL) was subjected to liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and about 17 peptides were identified, of which the RWDISQPY (1063.5 Da) was chosen to be synthesized based on in silico analysis. The RWDISQPY demonstrated high ACE inhibition ability (IC 50 : 72.24 µM) with competitive inhibition mode, and could effectively (p < 0.05) lower the systolic blood pressure and diastolic pressure of spontaneously hypertensive rats at the concentration of 150 mg/kg body weight. The results of the molecular docking simulation demonstrated that RWDISQPY could bind with the active sites S1 and S2 of ACE via short hydrogen bonds. Moreover, RWDISQPY showed acceptable endothelin-1 suppressing capacity (26.21% at 1.5 mg/mL). These results indicate that S. maclurei could be developed into functional foods such as antihypertensive products.

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Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 95%

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

Section: Inhibition Kinetics Of Synthetic Peptidesmentioning

confidence: 99%

See 2 more Smart Citations

Efficacy of a Novel ACE-Inhibitory Peptide from Sargassum maclurei in Hypertension and Reduction of Intracellular Endothelin-1

2020

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“…The above results further confirmed the report that the majority of ACE-inhibitory and antioxidant peptides are short sequences with 2-12 residuals, of which hydrophobic amino acids (Phe, Val, Leu, Ile, Pro, Ala, Trp, and Met) are the major ones [10,29]. Although the structural bioinformatics showed that ACE-inhibitory activity had a negative correlation with molecular mass of peptides, RGQVIYVL (946.6 Da) showed a higher activity than that of peptides identified from tilapia skin gelatin (VGLPNSR, IC 50 = 80.9 µM), horse gram protein (QLLLQQ, IC 50 = 75.0 µM), and rice bran protein (YSK, IC 50 : 76 µM) [25,30,31]. The reason may be the presence of Tyr and branch amino acids (Val and Leu) at the C-terminal of RGQVIYVL simultaneously.…”

Section: Characterization Of Pooled Peptide Fraction By Mass Spectrommentioning

confidence: 99%

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

et al. 2019

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Albumin is the major fraction of quinoa protein that is characterized as having high nutritional value. However, until now, scant information is available on the bioactivity of quinoa albumin or its hydrolysates. To promote its usage, we extracted albumin in this study from quinoa bran assisted with cellulase and hemicellulose, and hydrolyzed it by alcalase and trypsin to produce bioactive peptides. The hydrolysates (QBAH) were purified by gel filtration and reversed-phase high-performance liquid chromatography (RP-HPLC), followed by identification using liquid chromatography-mass spectrometry (LC-MS/MS). Furthermore, based on in silico analysis, one angiotensin-I converting enzyme (ACE)-inhibitory and antioxidant peptide, RGQVIYVL (946.6 Da), and two antioxidant peptides, ASPKPSSA (743.8 Da), and QFLLAGR (803.5 Da), from QBAH were synthesized. RGQVIYVL showed a high ACE-inhibitory activity (IC 50 = 38.16 µM) with competitive mode of inhibition, and showed significant antihypertensive effect in spontaneously hypertensive rats at a concentration of 100-150 mg/kg body weight (bw). Molecular docking simulation showed that it could interact with the active ACE site via hydrogen bonds with high binding power. Moreover, RGQVIYVL, ASPKPSSA, and QFLLAGR all demonstrated high ·OH scavenging activity (IC 50 = 61.69-117.46 µM), ABTS + scavenging activity (58.29-74.28%) and Fe 2+ chelating ability (32.54-82.48% at 0.5 mg/mL). They could also retain activity after gastrointestinal enzyme digestion. These results indicate that quinoa albumin is a potential source of bioactive peptides possessing antioxidant and ACE-inhibitory activities.

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Morphological traits of drought tolerant horse gram germplasm: classification through machine learning

et al. 2020

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BACKGROUND Horse gram (Macrotyloma uniflorum (Lam.) Verdc.) is an underutilized pulse crop with good drought resistance traits. It is a rich source of protein. Conventional breeding methods for high yielding and abiotic stress tolerant germplasm are hampered by the scarcity of morphological data sets. Thus, horse gram cultivars considered for this study is classified based on prevailing growth factors showing homogenous genotype in various agro ecological zones. Nowadays, several machine learning (ML) methods are used in the field of plant phenotyping. RESULTS We adopted unsupervised learning techniques from the K‐means clustering algorithm to analyze important morphological traits: plant shoot length, total plant height, flowering percentage, number of pods per plant, pod length, number of seeds per plant, and seed length variants between germplasm. Unsupervised clustering revealed that 20 germplasm accessions were grouped in four clusters in which high‐yielding traits were predominantly observed in cluster 2. CONCLUSION These findings could guide ML‐based classification to characterize suitable germplasms on the basis of high‐yielding varieties for different agro‐ecological zones. © 2020 Society of Chemical Industry

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Purification, identification, and characterization of novel angiotensin I-converting enzyme (ACE) inhibitory peptides from alcalase digested horse gram flour

Cited by 57 publications

References 29 publications

Efficacy of a Novel ACE-Inhibitory Peptide from Sargassum maclurei in Hypertension and Reduction of Intracellular Endothelin-1

Efficacy of a Novel ACE-Inhibitory Peptide from Sargassum maclurei in Hypertension and Reduction of Intracellular Endothelin-1

Isolation of Novel ACE-Inhibitory and Antioxidant Peptides from Quinoa Bran Albumin Assisted with an In Silico Approach: Characterization, In Vivo Antihypertension, and Molecular Docking

Morphological traits of drought tolerant horse gram germplasm: classification through machine learning

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