Purification of a Human Prostate Specific Antigen

Mc, Wang; La, Valenzuela; Gp, Murphy; Tm, Chu

doi:10.1016/s0022-5347(05)65270-6

Cited by 35 publications

(38 citation statements)

References 11 publications

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“…A recent study by Okabe et al [30] demonstrated that human Klk3, also known as prostate-specific antigen [31,32], degrades IGFBP-3 with a restricted chymotrypsin-like activity. In this sense, mKlk27 apparently resembles human Klk3.…”

Section: Discussionmentioning

confidence: 99%

Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity

2000

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A cDNA clone of a new mouse tissue kallikrein, designated mKlk27, was isolated from an adult mouse testis cDNA library. mKlk27 was expressed in the submaxillary glands and testis of the mouse. In testis, mKlk27 gene was expressed exclusively in the Leydig cells of the adult mouse. Active recombinant mKlk27 exhibited chymotrypsin-like cleavage specificity. A single amino-acid substitution of Gly for Asp at position 209 in mKlk27 resulted in complete loss of its chymotryptic activity but acquisition of tryptic activity. mKlk27 effectively hydrolyzed casein, gelatin and fibronectin. Insulin-like growth factor binding protein-3 was also hydrolyzed by recombinant mKlk27. These results suggest that mKlk27 plays an important role in association with the function of the adult mouse testis.

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Section: Discussionmentioning

confidence: 99%

Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity

2000

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“…Since the early identification of PSA [7] and proposal to use serum PSA as a first-line aid toward early detection of PCa [8], there has been a substantial dialogue among physicians, clinicians, and researchers regarding the cost-benefit of such screening. It is agreed that an appreciable number of clinical interventions resulting from "positive" PSA tests may be unnecessary, causing some patients unnecessary physical, emotional, and financial burdens [9].…”

Section: Introductionmentioning

confidence: 99%

PSA enzymatic activity: A new biomarker for assessing prostate cancer aggressiveness

Ahrens¹,

Bertin²,

Vonesh

et al. 2013

The Prostate

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“…The reported first cancer marker was the light chain of immunoglobulin in the urine, as identified in 75% of patients with myeloma. For example, CA-125 which has been proved to be a prognostic biomarker for ovarian epithelial cancer [14], carcinoembryonic antigen which has been proved to be the prognosis biomarker for colon cancer, and prostate-specific antigen which has been considered to be one of the best prostate cancer markers [15,16].…”

Section: The Role Of Biomarker In Diagnosis and Prognosis Of Osccmentioning

confidence: 99%

“…Also notable the family of calcium-binding proteins (calgranulin A, calgranulin B, S10 0 A14, calmod ulinlike protein), keratinocyte differentiation markers (involucrin, small pro-line-rich protein 3, profilin 1, cornifin A, cornifin B), and many membrane-related molecules (annexin A1, actin-like protein 2) were identified. A number of proteins including heat shock protein 27 (HSP27) (15), HSP70 (16), and GSH-Stransferase (15), expected to be of higher abundance in tumor samples, were identified. A number of signaling molecules (e.g., Ras GTPase-activating-like protein IQGAP1, obscurin, tyrosine-protein kinase, ITK) were also identified.…”

Section: Protein Biomarkers For Early Detection Of Osccmentioning

confidence: 99%

Potential biomarkers for oral squamous cell carcinoma: Proteomics discovery and clinical validation

Ding

et al. 2014

Proteomics Clinical Apps

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Oral squamous cell carcinoma (OSCC) is the worldwide concerned cancer. In spite of the advances in treatment, the 5-year survival rate has only increased subtly during the past two decades, which is largely due to the advanced stages of disease at diagnosis and the frequent development of relapse and second primary tumors. Therefore, the identification of underlying OSCC protein biomarker during cancer initiation and progression could aid the diagnosis and treatment of OSCC. In this review, recent researches on proteomics analysis of tissue, saliva, and serum for early detection and evaluation aggressiveness and occurrence of OSCC were summarized. The emphasis is placed on early detection by tissues, saliva, and serum of patients with histologically defined OSCC patients. Although lots of researches for searching OSCC protein biomarker have done, few common protein biomarkers have been detected. Low-redundant protein in tissues, saliva, and serum from OSCC may more accurately reflected the progression of OSCC, so novel approach for the depth research strategy and the sample choice for proteomics are of importance in OSCC biomarker discovery.

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Purification of a Human Prostate Specific Antigen

Cited by 35 publications

References 11 publications

Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity

Cloning and characterization of mouse Klk27, a novel tissue kallikrein expressed in testicular Leydig cells and exhibiting chymotrypsin-like specificity

PSA enzymatic activity: A new biomarker for assessing prostate cancer aggressiveness

Potential biomarkers for oral squamous cell carcinoma: Proteomics discovery and clinical validation

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