Purification of a lectin-like antifungal protein from the medicinal herb, Withania somnifera

Ghosh, Modhumita

doi:10.1016/j.fitote.2008.10.004

Cited by 26 publications

(17 citation statements)

References 42 publications

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“…In the past few years, scientists have isolated .70 legume lectins, mostly derived from seeds and now called the legume lectin family [6]. Many lectins are also found in other plant tissues, including leaves [7], rinds [8], rhizomes [9], and roots [10]. These lectins have been found to be highly homologous to the seed lectins.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Li¹,

Yin²,

Liu³

et al. 2012

ABBS

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Sophora alopecuroides lectin (SAL), a novel lectin from the seeds of Sophora alopecuroides, was purified by ionexchange chromatography on diethylaminoethyl (DEAE)-and carboxymethyl (CM)-Sepharose columns, followed by gel filtration on a Sephadex 75 10/300 GL column. SAL was found to be a monomer of 39916.3 Da, as determined by tricine-sodium dodecyl sulphate-polyacrylamide gel electrophoresis and high-performance liquid chromatography (HPLC). The N-terminal 10-amino acid sequence of SAL, KPWALSFSFG, resembles those of other legume lectins. SAL exhibits hemagglutinating activity against rabbit erythrocytes at 11.9 mg/ml. Its hemagglutinating activity is stable in the pH range 7-11 and in the temperature range 30 -908 8 8 8 8C, and is stimulated by Mn 21. The hemagglutinating activity of SAL is most potently inhibited by 50-mM D-galactose. SAL suppresses mycelial growth in Penicillium digitatum and Alternaria alternata; the IC 50 of the antifungal activity toward P. digitatum and A. alternata were found to be 3.125 and 3.338 mM, respectively. SAL suppresses the proliferation of human cervical cancer cells (HeLa) at an IC 50 of 6.25 mM (P < 0.05). But it has no inhibiting effect on bacteria. This is the first report of a lectin from seeds of S. alopecuroides.

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Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Li¹,

Yin²,

Liu³

et al. 2012

ABBS

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“…However, the AFP was not purified from A. giganteus using the isoelectric focusing so far. Meanwhile, many of antifungal protein have been isolated and purified by using the isoelectric focusing system [9]- [11]. The established purification protocol of AFP could be efficient and valuable in AFP purification and yield.…”

Section: Discussionmentioning

confidence: 99%

“…It is based on the principle that the protein placed in a pH gradient within an electric field will migrate toward either the cathode or anode until it reaches a pH at which there is no net charge on the protein molecule [9]- [11].…”

Section: Optimization Of Afp Purification From a Giganteus By Isoelementioning

confidence: 99%

“…Most recently 16.22 mg•AFP•l −1 were purified using Cellulose phosphate (Cph) as cation exchange chromatography and Sephadex G-50 with incubation the culture at 28˚C for about 100 hrs followed by an additional incubation period for 17 -20 hrs at 37˚C [8]. A few studies were established for purification different antifungal peptides applying the isoelectric focusing procedure [9]- [11], where no one used this technique for purification of AFP from A. gigenteus so far.…”

Section: Introductionmentioning

confidence: 99%

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Bio-Control of Alternaria alternata during Banana Storage by Purified AFP Using Isoelectric Focusing Technique

Barakat¹

2014

FNS

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Interestingly, antifungal protein AFP was purified from Aspergillus giganteus supernatants with modified isoelectric focusing procedure after adaptation of the secretion conditions. Subsequently, the antifungal activity as well as the mode of action against Alternaria alternata was tested in vitro. Moreover, different concentrations of AFP were applied to banana fruits for 15 days at 20˚C in vivo. Obtained results illustrated that A. giganteus was able to secrete about 39.78 ± 2.39 mg AFP•l −1 Olson medium. The employed ammonium sulfate (AS 75%) precipitation procedure followed by dialysis steps yielded about 16 -22 mg AFP•l −1 culture supernatant with general mean of 18.67 ± 1.98 mg•l −1 . The lost amount of AFP during purification using AS and 3KDa cut-off dialysis membrane is about 50% thus, purification procedure must be further improved. Indeed, concluded results from MIC and hyphal extension inhibition test noticed that AFP was efficiently affected by either growth or hyphae form of A. alternata in vitro. The MIC of AFP against A. alternata was 2 μg•ml −1 . However, short, thick and highly septated hyphae with damaged constricted apical regions extruding from condensed mycelium aggregates in treated hyphae compared to the untreated culture was remarkably shown. The mode of action of in vitro experiment manifested that AFP was effective to act the fungal cell and permeabilize the cell membrane of A. alternata. Furthermore, the in vivo experiment showed that AFP could reduce post-harvest decay on banana caused by A. alternata. AFP at concentration of 15 and 25 μg•ml −1 exhibit Alternaria decayed reduction by 45.45% and 77.27%, respectively. While no Alternaria decayed area was observed when 50 μg•ml −1 was applied during the storage time. Quantification of DNA by species-specific PCR could exude a positive correlation between the DNA amount and decayed area. In conclusion, AFP can be efficiently used as a bio-preservative agent during storage and handling of banana fruits, and considered as an excellent biological alternative to combat secondary growth of filamentous fungi.

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“…Its medicinal properties are due to withanolides, the characteristic secondary metabolites of this plant species (Matsuda et al, 2001). Few studies also show that the plant has herbicidal , and antifungal activates (Ghosh, 2009). However, studies regarding antifungal activity of W. somnifera against F. oxysporum f. sp.…”

Section: Introductionmentioning

confidence: 99%

Antifungal activity of methanolic root extract of Withania sommnifera against Fusarium oxysporum f. Sp. Cepae

Javaid¹,

Akhtar²

2015

Afr. J. Trad. Compl. Alt. Med.

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Background: Basal rot of onion (Allium cepa L.) caused by Fusarium oxysporum f. sp. cepae is a common soil-borne disease that causes significant yield losses. Generally, synthetic fungicides are used to combat the menace which causes environmental pollution. The present study was carried out to assess the antifungal activity of Withania somnifera (L.), Dunal, a Solanaceous medicinal plant, against the pathogen of this disease. Materials and Methods: Different concentrations (from 0.5 to 4%) of methanolic extract of root stem and fruit of the test plant species were prepared and their bioactivity was assessed against the target fungal pathogen. Methanolic extract of root was further fractionated with n-hexane, chloroform, ethyl acetate and n-butanol. A range of concentrations of these extracts viz. 200, 100… 3.125 mg mL -1 were prepared and assessed for their antifungal activities. Results: Methanolic root extract exhibited the best antifungal activity, causing up to 93% decrease in biomass of the fungal pathogen. n-hexane, chloroform and ethyl acetate fractions of methanolic root extract exhibited pronounced antifungal activity resulting in 46-79%, 40-73% and 35-76% reduction in fungal biomass respectively. Conclusion: The present study concludes that root extract of W. somnifera possesses potent antifungal constituents which can be used for the control of F. oxysporum f. sp. cepae.

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Purification of a lectin-like antifungal protein from the medicinal herb, Withania somnifera

Cited by 26 publications

References 42 publications

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Bio-Control of <i>Alternaria alternata</i> during Banana Storage by Purified AFP Using Isoelectric Focusing Technique

Antifungal activity of methanolic root extract of <i>Withania sommnifera</i> against <i>Fusarium oxysporum</i> f. Sp. <i>Cepae</i>

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Purification of a lectin-like antifungal protein from the medicinal herb, Withania somnifera

Cited by 26 publications

References 42 publications

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from &lt;italic&gt;Sophora alopecuroides&lt;/italic&gt; seeds

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from &lt;italic&gt;Sophora alopecuroides&lt;/italic&gt; seeds

Bio-Control of &lt;i&gt;Alternaria alternata&lt;/i&gt; during Banana Storage by Purified AFP Using Isoelectric Focusing Technique

Antifungal activity of methanolic root extract of <i>Withania sommnifera</i> against <i>Fusarium oxysporum</i> f. Sp. <i>Cepae</i>

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Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Isolation and characterization of a novel lectin with antifungal and antiproliferative activities from <italic>Sophora alopecuroides</italic> seeds

Bio-Control of <i>Alternaria alternata</i> during Banana Storage by Purified AFP Using Isoelectric Focusing Technique