Purification of Adrenocorticotropic Peptides by Carrier Displacement Chromatography

INFLUENCES OF PROTEOLYTIC ENZYMES ON PAROTIN VII. ISOLATION AND SOME PROPERTIES OF PAROTIN-T. B<SUB>1</SUB> AND PAROTIN-T. B<SUB>2</SUB>

“…The B1-fraction precipitated readily at the concentration of 5% of TCA as shown in Figure 1, while no more precipitate was produced by increasing the TCA-concentration of the solution. Li et al(1952) reported that the adrenocorticotropic peptides prepared by peptic digestion of sheep ACTH protein was purified by fractional precipitation with TCA,and the activity in the digest was found in the precipitate at the concentration from 5 to 25% of TCA.In contrast with the result of ACTH peptide, the precipitate at the concentration from 5 to 25% of TCA was not obtained in author's experiment of tryptic digest of parotin. However, on the other hand, the precipitate (B2-fraction) could be obtained by the addition of ether-acetone mixture (1: 1) to the TCA supernatant.…”

Biochemical Analysis

“…The gonadotropic proteins from pregnancy urine have been adsorbed on kaolin, eluted, and chromatographed on calcium phosphate gel (108). ACTH peptide mixtures have been purified with active carbon and carrier displacement technique (301), but more general use of oxycellulose columns has been made (28, 53, 164, 414)• Richter et al (414) were able to use buffered IRC 50 resin to effect some purification of their ACTH preparation. The resins of low cross linkage should replace oxycellulose as an adsorbent for peptides, especially when a carboxylic resin becomes available in this form.…”

Adsorption Studies of Enzymes and Other Proteins

“…Certain individual enzymes and viruses are discussed in detail later, as well as the many examples of filter paper chromatography of enzymes and other proteins. A number of interesting studies on polypeptides have appeared recently-gramicidin (208), peptides (97,158), tissue peptides (52), ACTH peptides (113,114), licheniformins (mol. wts.…”