Purification of Allergens in Cladosporium herbarum

10 strains (isolates) of Aspergillus fumigatus, grown and harvested under identical conditions, were compared with respect to biochemical and immunological properties. The total protein and carbohydrate content and isoelectric focusing pattern were determined for each of the individual strains. Their antigenic and allergenic activity was examined by a combination of crossed immunoelectrophoresis (CIE)/crossed radioimmunoelectrophoresis (CRIE) and RAST inhibition. Biochemical and immunochemical data indicated that considerable variation exists between the various isolates of A. fumigatus. 44 antigens were demonstrated in CIE of A. fumigatus, and 18 different allergens were identified in CRIE (2 major, 10 intermediate and 6 minor allergens).

Section: Discussionmentioning

confidence: 90%

Section: Crossed Radioimmunoeleciropltoresis (Crie/mentioning

confidence: 99%

Antigenic Variability of Different Strains of Aspergillus fumigatus

Wallenbeck

Aukrust²,

Einarsson

1984

“…Twenty-two patient sera with suspected allergy to A. altérnala (selected by RAST results and by history in combination with SPT) were used for classification of the individual allergens of A. altér nala extracts into minor, intermediate and major allergens [4], A pool of 2 of these broad-spectrum patient sera (5726 and 5734) was used for studying strain variability in detail.…”

Section: Patient Seramentioning

confidence: 99%

“…Indirect autoradiography was performed at -70°C using Du Pont Cronex-4 X-ray films in Kodak Cassettes with Du Pont light ing plus intensifying screen [14], By applying a CRIE classification system based upon exposure time necessary for visible radiostaining [2], allergens may be defined as major, intermediate and minor [4], CRIE class A means dense radiostaining, while CRIE class G corresponds to weak but specific radiostaining. A mixture of equal amounts of all individual isolates was defined as 100% relative potency in RAST inhibition analysis.…”

Section: Crossed Radioimmunoelectrophoresis (Crie)mentioning

confidence: 99%

Variability of Antigenicity/Allergenicity in Different Strains of Alternaria alternata

Steringer

Aukrust²,

Einarsson

1987

Biochemical and immunological properties were studied in crude mould extracts from 10 different strains of Alternaria alternata. The mould strains were grown on completely synthetic medium and harvested under identical conditions. The biochemical and immunological analyses (protein content, carbohydrate content, IEF, RAST-inhibition, CIE/CRIE and the content of a major allergen) showed significant variations between the individual strains of A. alternata. 32 antigens were identified in the CIE pattern of A. alternata and 19 different allergens were identified in CRIE analysis (1 major, 7 intermediate and 6 minor allergens). There was a significant variation in the content of the major A. alternata allergen (Ag-8, partially identical to Alt-I) between the different individual strains. Furthermore, a considerable variation in the content of Ag-8 was observed for a specific strain cultivated at different occasions under identical cultivation conditions during a time period of 3 years.

“…All plates were exposed several times, using time periods varying from I to 27 h (11 ]. Each exposure corre sponded to one particular CRIE class, using the CRIE classifica tion system previously described [12,13]. As a negative control, a patient serum (8369) not sensitive to rye pollen proteins was uti lized (Phadebas Rast class 0, total IgE 16.3 kU/1).…”

Section: Crossed Radioimmunoelectrophoresis(crie)mentioning

confidence: 99%

Crossed Radioimmunoelectrophoretic Analysis of Cultivated Rye (Secale cereale) Pollen Allergens

Renck¹,

Einarsson²

1984

Cultivated rye pollen extracts were characterized by means of. crossed immunoelectrophoresis (CIE) and crossed radioimmunoelectrophoresis (CRIE). 32 antigens were identified in CIE and among these 16 were radiostained in CRIE analysis of a rye pollen sensitive patient panel. Crossed line immunoelectrophoresis revealed that some of the rye pollen antigens were immunological partially identical with antigens of wheat flour and rye fluor.