Purification of antibiotics produced byLentinus squarrosulus and preliminary characterization of a compound active againstRigidoporus lignosus

Sudirman, Lisdar Idwan; Lefèbvre, G.; Kiffer, E.; Botton, Bernard

doi:10.1007/bf01570183

Cited by 15 publications

(8 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The water soluble glucans from L. squarrosulus have immune-enhancing properties [7], while water soluble extract from mycelia eliminated ulcer in rats within 72 hours [6]. Other investigators reported its potential as a biocontrol agent against Rigidoporus lignosus, a fungal pathogen of rubber, and an antimicrobial against Bacillus subtilis, Mucor ramannianus and yeast [4].…”

Section: Introductionmentioning

confidence: 99%

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Anike¹,

Isikhuemhen²,

Blum³

et al. 2015

ABB

View full text Add to dashboard Cite

Lentinus squarrosulus Mont. is an emerging tropical white rot basidiomycete, with nutritional and medicinal benefits. Low levels of commercial cultivation of the mushrooms limit their availability for use as food and medicine. Mycelia from submerged fermentation are a suitable alternative to the mushroom from L. squarrosulus. Three strains, 340, 339 and 218, were studied to determine optimum growth conditions for mycelia mass and crude exo-polysaccharides (CEPS) production. The experiments were conducted in a completely randomized design (CRD) with a factorial structure. Nutrients involving 8 carbon and 8 nitrogen sources were screened, and concentrations of the best sources were optimized. Optimized nutrients, interaction between strains and other parameters such as agitation and medium volume were investigated to obtain optimum fermentation conditions for biomass and CEPS production. Biomass yield varied among strains depending on carbon or nitrogen nutrient sources. Starch and yeast extract at 30 and 25 g/L were identified as the most important nutrients in mycelia and CEPS production. Nutrient optimization resulted in a 3-fold increase in mycelia mass: 12.8, 10.0 and 15.3 g/L in strains 340, 339 and 218 respectively. There was a significant interaction between strain, agitation, and volume (p < 0.001). Mycelia mass increased with volume under shake conditions, while polysaccharides decreased. There was a weak and negative correlation between mycelia mass and polysaccharides (p = 0.02). Static conditions favored more polysaccharide production. Optimized fermentation conditions resulted in very high increase in biomass: 238.1, 266.9 and 185.0 g/L in strains 340, 339 and 218 respectively. Results obtained could be useful in modeling fermentation systems for large-scale production of mycelia mass, CEPS and other bio-products from L. squarrosulus.

show abstract

Section: Introductionmentioning

confidence: 99%

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Anike¹,

Isikhuemhen²,

Blum³

et al. 2015

ABB

View full text Add to dashboard Cite

show abstract

“…A literature survey showed that only a few Lentinus sp. have been investigated as sources of natural bioactive products and effects; these include the antibiotic activity found in L. degener and L. squarrosullus (Sudirman et al 1994), the antithrombotic activity of L. adhaerens (Lauer et al 1991), and the cytotoxic and antimalarial activities of L. connatus (Rukachaisirikul et al 2005).…”

Section: Resultsmentioning

confidence: 99%

“…CCB culture isolates were collected in Brazilian states of Alagoas, Mato Grosso Sul, andSão Paulo between March 1992 and1994. These isolates were previously identified in the Basidiomycete Culture Collection of the Instituto of the Botânica of São Paulo state, Brazil.…”

Section: Collection and Identificationmentioning

confidence: 99%

Cytotoxic, immunosuppressive, trypanocidal and antileishmanial activities of Basidiomycota fungi present in Atlantic Rainforest in Brazil

Rosa

Machado

Rabello

et al. 2009

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

One hundred three Basidiomycota fungi representing 84 species and 17 families were collected from different Atlantic Rainforest in Brazil. Their basidiomes and fermentation broth extracts were screened in a bioassay panel that included three human cancer cells lines, human peripheral blood mononuclear cells (PBMCs), the enzyme trypanothione reductase (TryR) from Trypanosoma cruzi, and amastigote forms of Leishmania amazonensis. Forty-two extracts representing 21 genera and 35 species presented activities higher than 60% in one or more assays employed in this study. Eighteen extracts were toxic to one or more human cancer cell lines. Extracts from Lentinus strigosus CCB 178 and Lentinus sp. UFMGCB 38 showed selectivity towards cancer cells as they showed only a minor impact on PBMCs. Six extracts suppressed PBMCs proliferation and showed low toxic effect to cancer cells. Thirty-four extracts inhibited the activity of the TryR. Of these, five showed low toxicity towards PBMCs. Extracts from Gymnopilus areolatus, Irpex lacteus, L. strigosus, Nothopanus hygrophanus, Pleurotus flabellatus, and unidentified Basidiomycetes were toxic to L. amazonensis. The results of this screening reinforce the potential of Basidiomycota fungi as sources of bioactive natural products that may be developed into new therapeutic agents for cancer and neglected diseases such as trypanosomiasis and leishmaniasis.

show abstract

“…Chemical studies on a large-scale fermentation broth of BCC 22366 afforded a new tetrahydrobenzofuran derivative 1, along with the known 1,2-dihydroxymintlactone (2) (Stadler et al, 1995). There was a report of two antibiotic compounds from L. squarrosulus, although their structures have not been elucidated (Sudirman et al, 1994 showed HMBC correlation to the ketone carbon (C-8), which indicated an isovaleroyl group (C-8-C-12). COSY data also demonstrated the connection of two other methylenes (CH 2 -4 and CH 2 -5), which constituted a part of the six-membered ring.…”

Section: Introductionmentioning

confidence: 97%

A tetrahydrobenzofuran derivative from the fermentation broth of Lentinus squarrosulus BCC 22366

Isaka

Sappan

Rachtawee

et al. 2011

Phytochemistry Letters

View full text Add to dashboard Cite

Purification of antibiotics produced byLentinus squarrosulus and preliminary characterization of a compound active againstRigidoporus lignosus

Cited by 15 publications

References 27 publications

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Cytotoxic, immunosuppressive, trypanocidal and antileishmanial activities of Basidiomycota fungi present in Atlantic Rainforest in Brazil

A tetrahydrobenzofuran derivative from the fermentation broth of Lentinus squarrosulus BCC 22366

Contact Info

Product

Resources

About

Purification of antibiotics produced byLentinus squarrosulus and preliminary characterization of a compound active againstRigidoporus lignosus

Cited by 15 publications

References 27 publications

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by &lt;i&gt;Lentinus squarrosulus&lt;/i&gt;

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by &lt;i&gt;Lentinus squarrosulus&lt;/i&gt;

Cytotoxic, immunosuppressive, trypanocidal and antileishmanial activities of Basidiomycota fungi present in Atlantic Rainforest in Brazil

A tetrahydrobenzofuran derivative from the fermentation broth of Lentinus squarrosulus BCC 22366

Contact Info

Product

Resources

About

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>

Nutrient Requirements and Fermentation Conditions for Mycelia and Crude Exo-Polysaccharides Production by <i>Lentinus squarrosulus</i>