Purification of bubaline luteinizing hormone by gel filtration chromatography in the presence of blue dextran

Chaudhary, Rajesh; Jain, Shuchi; Muralidhar, K.; Gupta, Munishwar N.

doi:10.1016/j.procbio.2005.10.003

Cited by 17 publications

(11 citation statements)

References 18 publications

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“…Haeckel et al., to the best of our knowledge, were the first to make use of the tendency of blue dextran to bind certain proteins; they used it for the purification of yeast pyruvate kinase . This technique was also utilized for the purification of other proteins including human erythrocyte pyruvate kinase and bubaline luteinizing hormone …”

Section: Anthraquinones As Biochemical and Pharmacological Tools: Reamentioning

confidence: 99%

“…147 This technique was also utilized for the purification of other proteins including human erythrocyte pyruvate kinase 154,155 and bubaline luteinizing hormone. 156 Affinity chromatography, or what is also known as dye-ligand chromatography, 120 differs from the gel filtration technique in that blue dextran is fixed to the adsorbent system to trap the protein of interest out of the crude sample. Elution of the protein is then achieved as in gel filtration chromatography ( Fig.…”

Section: A Role Of Reactive Blue 2 In Protein Purificationmentioning

confidence: 99%

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Anthraquinones As Pharmacological Tools and Drugs

Malik

Müller

2016

Medicinal Research Reviews

358

211

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Anthraquinones (9,10-dioxoanthracenes) constitute an important class of natural and synthetic compounds with a wide range of applications. Besides their utilization as colorants, anthraquinone derivatives have been used since centuries for medical applications, for example, as laxatives and antimicrobial and antiinflammatory agents. Current therapeutic indications include constipation, arthritis, multiple sclerosis, and cancer. Moreover, biologically active anthraquinones derived from Reactive Blue 2 have been utilized as valuable tool compounds for biochemical and pharmacological studies. They may serve as lead structures for the development of future drugs. However, the presence of the quinone moiety in the structure of anthraquinones raises safety concerns, and anthraquinone laxatives have therefore been under critical reassessment. This review article provides an overview of the chemistry, biology, and toxicology of anthraquinones focusing on their application as drugs.

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Section: Anthraquinones As Biochemical and Pharmacological Tools: Reamentioning

confidence: 99%

Section: A Role Of Reactive Blue 2 In Protein Purificationmentioning

confidence: 99%

Anthraquinones As Pharmacological Tools and Drugs

Malik

Müller

2016

Medicinal Research Reviews

358

211

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“…The method exploited differential binding of LH (in the crude extract) to blue dextran for the fi rst chromatography step. Before the second step, addition of high salt released LH from the blue dextran, enabling effective purifi cation [ 6 ]. Fusion ferritin (heavy-chain ferritin plus light-chain ferritin) has also been purifi ed by urea-gradient gel fi ltration.…”

Section: Column Cleaning and Storagementioning

confidence: 99%

Gel-Filtration Chromatography

Ó’Fágáin

Cummins

O’Connor

2016

Methods in Molecular Biology

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“…The method exploited differential binding of LH (in the crude extract) to blue dextran for the first chromatography step. Before the second step, addition of high salt released LH from the blue dextran, enabling effective purification (4). Fusion ferritin (heavy-chain ferritin plus light-chain ferritin) has also been purified by urea-gradient gel-filtration.…”

Section: Applications Of Gel-filtration Chromatographymentioning

confidence: 99%