Purification of chloroplast elongation factors

Tiboni, Orsola; Ciferri, Orio

doi:10.1016/0076-6879(86)18080-3

Cited by 4 publications

(3 citation statements)

References 10 publications

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“…The results showed that the two proteins involved in translation process were downregulated; the putative elongation factor 3 (EF-3) (PHATRDRAFT_27838), which facilitates EF-1-alpha-dependent binding with aminoacyl-tRNA to the ribosome, thus resulting in the transduction of mechanical energy from nucleoside triphosphate energy for translocation during translation 57 and chloroplast elongation factor Ts, (EFTS_PHATC), which associates with the EF-Tu-GDP complex and remains bound to the aminoacyl-tRNA, thus facilitating the conversion of GDP to GTP 58 . This showed that the translation levels of proteins decreased after 48 h of –P starvation.…”

Section: Resultsmentioning

confidence: 99%

Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum

Feng

Yang

Zheng

et al. 2015

Sci Rep

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Phosphorus (P) is an essential macronutrient for the survival of marine phytoplankton. In the present study, phytoplankton response to phosphorus limitation was studied by proteomic profiling in diatom Phaeodactylum tricornutum in both cellular and molecular levels. A total of 42 non-redundant proteins were identified, among which 8 proteins were found to be upregulated and 34 proteins were downregulated. The results also showed that the proteins associated with inorganic phosphate uptake were downregulated, whereas the proteins involved in organic phosphorus uptake such as alkaline phosphatase were upregulated. The proteins involved in metabolic responses such as protein degradation, lipid accumulation and photorespiration were upregulated whereas energy metabolism, photosynthesis, amino acid and nucleic acid metabolism tend to be downregulated. Overall our results showed the changes in protein levels of P. tricornutum during phosphorus stress. This study preludes for understanding the role of phosphorous in marine biogeochemical cycles and phytoplankton response to phosphorous scarcity in ocean. It also provides insight into the succession of phytoplankton community, providing scientific basis for elucidating the mechanism of algal blooms.

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Section: Resultsmentioning

confidence: 99%

Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum

Feng

Yang

Zheng

et al. 2015

Sci Rep

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“…EF-Tu is an abundant soluble protein and has been purified from spinach [ 31 ] and Euglena gracilis [30]. Hybridization analysis using the E. coli EF-Tu gene (tufA) first suggested the existence of a gene encoding chloroplast EF-Tu in the chloroplast genome of Chlamydomonas reinhardtii [33].…”

Section: Introductionmentioning

confidence: 99%

Purification of chloroplast elongation factor Tu and cDNA analysis in tobacco: the existence of two chloroplast elongation factor Tu species

et al. 1993

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We have purified a chloroplast elongation factor Tu (EF-Tu) from tobacco (Nicotiana tabacum) and determined its N-terminal amino acid sequence. Two distinct cDNAs encoding EF-Tu were isolated from a leaf cDNA library of N. sylvestris (the female progenitor of N. tabacum) using an oligonucleotide probe based on the EF-Tu protein sequence. The cDNA sequence and genomic Southern analyses revealed that tobacco chloroplast EF-Tu is encoded by two distinct genes in the nuclear genome of N. sylvestris. We designated the corresponding gene products EF-Tu A and B. The mature polypeptides of EF-Tu A and B are 408 amino acids long and share 95.3% amino acid identity. They show 75-78% amino acid identity with cyanobacterial and chloroplast-encoded EF-Tu species.

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“…However, higher plant chloroplast EF-Tu, which shows higher sequence homology to bacterial elongation factor than to cytoplasmic elongation factor, is encoded by nuclear DNA (Baldauf and Palmer, 1990;Baldauf et al, 1990;Bonny and Stutz, 1993). Chloroplast EF-Tu is an abundant soluble protein and has been purified from spinach (Tiboni et al, 1978), E. gracilis (Tiboni and Ciferri, 1986), and Nicotiana tabacum (Murayama et al, 1993). A single nuclear tufA gone was identified in Arabidopsis thaliana (Baldauf and Palmer, 1990) and multiple copies of this gene seemed to exist in the nuclear genome of other Brassicaceae (Baldauf et al, 1990).…”

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confidence: 98%

Gene expression of chloroplast translation elongation factor Tu during maize chloroplast biogenesis

et al. 1997

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Purification of chloroplast elongation factors

Cited by 4 publications

References 10 publications

Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum

Examination of metabolic responses to phosphorus limitation via proteomic analyses in the marine diatom Phaeodactylum tricornutum

Purification of chloroplast elongation factor Tu and cDNA analysis in tobacco: the existence of two chloroplast elongation factor Tu species

Gene expression of chloroplast translation elongation factor Tu during maize chloroplast biogenesis

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