Purification of commercial preparations of NADP+ from AMP contamination

Combining the Power of Biocatalysis and Membrane-Based Purification To Access NADP+

“…However, it seems obvious that the purification process by ion exchange chromatography requires the use of certain chemicals such as LiCl, NaOH, HCl, solvents such as ethanol or methanol, and a large quantity of buffer which induces an important desalting step. 11,12,29,30 On the contrary, the purification by membrane filtration highlighted in this study does not require any organic solvent but water. Moreover, the water used in the membrane filtration process can be reused and recycled.…”

“…7−10 Despite the fact that this system allows to obtain a remarkable purity rate, it requires the use of an organic solvent and a large amount of water to wash the salts generated during the process. 11,12 The use of this purification method is particularly challenging due to the byproducts of the NADP + biocatalytic production pathway (Figure 1). 13,14 Indeed, three of the byproducts�adenosine monophosphate (AMP), adenosine diphosphate (ADP), as well as residual adenosine triphosphate (ATP) from the first step�have a very similar ionic profile to NADP + and high salt concentrations are needed to efficiently separate them from the wanted product.…”

“…Most oxidoreductases, which represent one of the largest classes of enzymes (25% of all known enzymes), are of significant interest but require the cofactor NADP­(H) or NAD­(H) for their catalytic activity. Despite this drawback, much effort is put into the development of these systems as they are able to promote bio oxido-reduction reactions that are vital for the global pharmaceutical and chemical market due to their enantioselectivity and intrinsic specificity. , Although the synthesis and regeneration methods of these cofactors are being developed, one of the current constraints concerns the purification processes of these molecules, which are poorly described in the literature and generally based on ion exchange chromatography. − Despite the fact that this system allows to obtain a remarkable purity rate, it requires the use of an organic solvent and a large amount of water to wash the salts generated during the process. , The use of this purification method is particularly challenging due to the byproducts of the NADP + biocatalytic production pathway (Figure ). , Indeed, three of the byproductsadenosine monophosphate (AMP), adenosine diphosphate (ADP), as well as residual adenosine triphosphate (ATP) from the first stephave a very similar ionic profile to NADP + and high salt concentrations are needed to efficiently separate them from the wanted product.…”