Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses

Stéhelin, D; Guntaka, Ramareddy V.; Varmus, Harold; Bishop, J. Michael

doi:10.1016/0022-2836(76)90152-2

Cited by 249 publications

(119 citation statements)

References 33 publications

Supporting

Mentioning

114

Contrasting

Unclassified

Order By: Relevance

“…Our results provide evidence that strongly suggests that rASVs have been generated by recombination between td viral and cellular src sequences which have been identified in normal chicken and other avian cells (18,19,20 (12,19,21 …”

Section: Discussionsupporting

confidence: 59%

Recombination between viral and cellular sequences generates transforming sarcoma virus

Wang¹,

Halpern²,

Nadel³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

A series of sarcoma viruses has been obtained from tumors induced by transformation-defective (td) mutants of the Schmidt-Ruppin strain of Rous sarcoma virus, subgroup A (SR-A). The RNA sequences of these “recovered avian sarcoma viruses” (rASVs) were compared with those of td mutants and of SR-A by oligonucleotide fingerprinting. Of six sarcoma-specific oligonucleotides present in SR-A RNA, three to six were missing in the RNAs of the four td mutants examined. All six isolates of rASV examined have regained these six oligonucleotides. In addition, most rASV RNAs have three new oligonucleotides not present in the RNA either of td mutants or of SR-A. The newly obtained oligonucleotides are located between 800 and 2600 nucleotides from the 3′ end of rASV RNA, which corresponds to the src region of SR-A RNA mapped previously. Furthermore, viral RNAs of two td mutants isolated from a clone of rASV lack most src -specific oligonucleotides, including the three new ones. No differences were found among RNAs of td, SR-A, and rASV in the regions outside of src . Our results indicate that RNA sequences that rASVs have acquired from cells in the process of conversion from td virus to transforming virus are mapped within the src region and segregate with the transforming function. Some of the sequences are new and some are identical with those in SR-A RNA.

show abstract

Section: Discussionsupporting

confidence: 59%

Recombination between viral and cellular sequences generates transforming sarcoma virus

Wang¹,

Halpern²,

Nadel³

et al. 1978

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…Then the first oncogene was finally described: SRC (for 'sarcoma'), a gene responsible for tumor induction upon integration into the host genome (Stehelin et al, 1976).…”

Section: The Warburg Effectmentioning

confidence: 99%

Metabolic reprogramming of the tumor

2012

View full text Add to dashboard Cite

Cancer is classically considered as a genetic and, more recently, epigenetic multistep disease. Despite seminal studies in the 1920s by Warburg showing a characteristic metabolic pattern for tumors, cancer bioenergetics has often been relegated to the backwaters of cancer biology. This review aims to provide a historical account on cancer metabolism research, and to try to integrate and systematize the metabolic strategies in which cancer cells engage to overcome selective pressures during their inception and evolution. Implications of this renovated view on some common concepts and in therapeutics are also discussed.

show abstract

“…Stehelin et al (11) isolated radioactive DNA (cDNAsarc) complementary to most or all of the viral gene(s) (src) required for transformation of fibroblasts by avian sarcoma virus (ASV) (11)(12)(13).…”

mentioning

confidence: 99%

“…In the previous study (10) [3H]cDNAsarc have been described (11). For preparation of cDNAsa0c, the genome of Prague C strain of ASV was transcribed into complementary DNA; hybridization was then used to select for DNA specific for the region missing from the genome of a transformation-defective (td) deletion mutant of Pr-C of ASV.…”

mentioning

confidence: 99%

Nucleotide sequences related to the transforming gene of avian sarcoma virus are present in DNA of uninfected vertebrates.

Spector¹,

Varmus²,

Bishop³

1978

Proc. Natl. Acad. Sci. U.S.A.

206

View full text Add to dashboard Cite

We have detected nucleotide sequences related to the transforming gene of avian sarcoma virus (ASV) in the DNA of uninfected vertebrates. Purified 1 for review). The rate of evolution of such sequences has been estimated from the final extent of annealing and thermal stability of duplexes formed between cellular DNA and virus-specific reagents. In general, virusspecific DNA in cellular genomes appears to evolve at least as rapidly as cellular unique-sequence DNA; this pattern has been documented for viral DNA endogenous to primates (2), cats (3), rodents (4), and birds (5-10). In some instances, the apparently rapid evolution of virus-specific DNA could be explained by its relatively recent introduction into the germ line of a species by infection with a virus originating in another species (3).In the cases cited, the hybridization reagents were not specific for defined portions of the viral genome, and the endogenous viruses under study were, in most cases, not oncogenic. Stehelin et al. (11) isolated radioactive DNA (cDNAsarc) complementary to most or all of the viral gene(s) (src) required for transformation of fibroblasts by avian sarcoma virus (ASV) (11-13).cDNA,.rc annealed to DNA not only from normal chickens, the presumed natural host for ASV, but also to DNA from several other birds spanning 100 X 106 years of evolution (10). This apparent conservation of nucleotide sequences related to src (hereafter called "sarc sequences") during avian speciation contrasted sharply with the lack of conservation of sequences encoding a nontransforming virus endogenous to chickens and closely related to ASV. Moreover, the conservation of cellular sarc sequences suggested that they might have an important, but as yet unknown, function, and that they might be present, albeit in a diverged form, in vertebrates other than birds.In the previous study (10) (14). Homogenized tissue or cells were suspended in buffer containing 0.1 M NaCI, 10 mM Tris-HCI (pH 7.4), 1 mM EDTA, 0.5% sodium dodecyl sulfate (NaDodSO4), and 500 ,ug of Pronase per ml at 370 for 1-2 hr and extracted with phenol/chloroform/isoamyl alcohol (25:24:1 vol/vol). The high molecular weight DNA was then spooled out of solution after addition of ethanol. High molecular weight salmon sperm and calf thymus DNA (Worthington Biochemical Corp.) were treated as above.The DNA was reduced to 6-7 S and residual RNA was digested by boiling in 0.3 M NaOH for 20 min. The samples were then neutralized with HCl, adjusted to 0.5% NaDodSO4, treated with 500 ,ug of Pronase per ml for 45 The publication costs of this article were defrayed in part by page charge payment. This article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.

show abstract

Purification of DNA complementary to nucleotide sequences required for neoplastic transformation of fibroblasts by avian sarcoma viruses

Cited by 249 publications

References 33 publications

Recombination between viral and cellular sequences generates transforming sarcoma virus

Recombination between viral and cellular sequences generates transforming sarcoma virus

Metabolic reprogramming of the tumor

Nucleotide sequences related to the transforming gene of avian sarcoma virus are present in DNA of uninfected vertebrates.

Contact Info

Product

Resources

About