Purification of flavonoids from Carex meyeriana Kunth based on AHP and RSM: Composition analysis, antioxidant, and antimicrobial activity

Cheng, Xianhe; Cheng, Yin-Wei; Zhang, Nijia; Zhao, Shuang; Cui, Hao; Zhou, Hongli

doi:10.1016/j.indcrop.2020.112900

Cited by 31 publications

(20 citation statements)

References 37 publications

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“…To evaluate the statistically significant difference in this model, Fisher’s test and p -value were conducted. The model F -value of 175.99 indicated that this mode is significant [ 28 ]. The p -value < 0.05 indicated that the model terms were significant.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products

Cho

Kim³

et al. 2020

Plants

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Currently, many extracts from natural sources are added to cosmetic products for reducing facial aging and wrinkles. This study investigated the antiwrinkle activity of enriched extract of Isatidis Folium used for a novel antiwrinkle cream product. The result demonstrated that this enriched extract has excellent antiwrinkle activity by significantly inhibiting mRNA expression of matrix metalloproteinase-1, matrix metalloproteinase-3, and pro-inflammatory cytokines IL-1β and upregulating the mRNA expression of IL-4 and procollagen. Additionally, to implement effective quality control of the entire manufacturing process of antiwrinkle cream products based on the enriched extract of Isatidis Folium, the main chemical constituents of the enriched extract of Isatidis Folium was evaluated by high–performance liquid chromatography-photodiode array-tandem mass spectrometry (HPLC-PDA-ESI-MS/MS), five constituents were undisputedly confirmed. An HPLC-UV method in 15-min analysis time for quality assessment of the entire manufacturing process of antiwrinkle cream products was proposed and validated. The optimal conditions for extracting TMCA (3,4,5-trimethoxycinnamic acid) from the developed antiwrinkle cream products were determined using response surface methodology based on central composite design. The established HPLC method and optimal extract condition are suitable for routinely analyzing this novel antiwrinkle cream product.

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Section: Resultsmentioning

confidence: 99%

Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products

Cho

Kim³

et al. 2020

Plants

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“…Biomolecules and extracts of other sources also demonstrated activity for scavenging hydroxyl radicals. Cheng et al [50] showed the activity of flavonoids from Carex meyeriana on scavenging the hydroxyl radical. Melocactus zehntneri ethanolic extract containing phenolic compounds, saponins and polysaccharides showed 100% hydroxyl scavenging activity for polar extract (using 100 µg/mL), whereas the non-polar extracts showed an activity of around 20% [46].…”

Section: Discussionmentioning

confidence: 99%

In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

et al. 2020

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Agave sisalana agro-industrial residue has considerable potential against damage associated with oxidative stress and skin aging. This study aims to demonstrate, in vitro and in vivo, the potential of Agave sisalana agro-industrial residue as a safe and effective alternative for the prevention of damage caused by oxidative stress and aging. The antioxidant activity was evaluated in vitro (total antioxidant capacity, reducing power, DPPH radical scavenging, metal chelating (Fe2+ and Cu2+), and hydroxyl radical scavenging) and in vivo using the Caenorhabditis elegans organism model. The extract showed in vitro antioxidant activity in all tests performed. Tests with C. elegans showed that the extract was able to reduce the intracellular levels of reactive oxygen species (ROS) and increase the survival rate of worms. A downregulation of gst-4::GFP expression suggests a direct action against free radicals. Agave sisalana agro-industrial residue extract (AsRE) can therefore be considered as a source of antioxidant biomolecules, and the use of this agro-industrial residue in a new production process can lead to sustainability and socioeconomic development.

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“…Ultra performance liquid chromatography (UPLC) analysis was performed on a UPLC-Orbitrap-MS system (Thermo, USA) with a Waters HSS T3 column (50 × 2.1 mm, 1.8 μm; Waters, Milford, USA). The determination of chemical compounds of PBAP had been slightly adjusted on the basis of previous literature (Cheng et al, 2020). The mobile phase was composed of different proportions of 0.1% formic acid solution (A) and 0.1% formic acid-acetonitrile-isopropyl alcohol solution (B) at a flow velocity of 0.3 ml/min and a column temperature of 40°C.…”

Section: Uplc-ms Analysismentioning

confidence: 99%

Composition analysis and antioxidant activity of purified Boletus auripes pigment using macroporous resin

Guo

Qiu

Zhou

et al. 2022

Food Processing Preservation

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Fungi pigment has been widely used in the food industry due to their high safety and various physiological activities, however, study on the Boletus auripes pigment (BAP) has rarely been reported. The aim of the present study was to optimize the purification process of BAP using macroporous resin. Moreover, the chemical composition and antioxidant activity of the purified BAP (PBAP) were also evaluated. The optimal purification parameters for the resin of XDA‐7 were as follows: at pH 6.0, the concentration of BAP was 2.0 mg/ml (30 ml) with a loading flow rate of 2.0 ml/min, and 80% ethanol was used as the eluent solvent with the elution flow rate of 1.5 ml/min. The results showed that the purity of PBAP after purification by XDA‐7 was 5.8 times higher than that of BAP. Fourier transform‐infrared spectrometry showed that the absorption peaks of PBAP were associated with the signals of the aldoketones functional group. Ultra performance liquid chromatographytandem‐mass spectrometry indicated that there were 32 components were identified from the PBAP, including 15 organic acids, 9 glycosides, 3 esters, 3 vitamins, and 2 alkaloids. The scavenging capacity of DPPH free radical (DPPH·), ABTS free radical (ABTS+.), Hydroxyl free radical (·OH), and the reducing power was measured to investigate the antioxidant capacity of BAP and PBAP. The obtained results showed that the two pigments possessed good antioxidant capacity with the dose–effect relationship. The IC50 values of PBAP for scavenging DPPH·, ABTS+., and ·OH were 0.048, 0.001, and 0.31 mg/ml, respectively, and they were much higher than the corresponding results that showed 0.103, 0.049, and 1.295 mg/ml of the BAP. Obviously, the antioxidant activity of BAP was significantly enhanced after purification. In short, the study provided a reference for the further development and utilization of the pigment from B. auripes. Novelty impact statement As a new source of natural pigments, Boletus auripes pigment (BAP) has not attracted the attention of researchers. In this article, BAP was purified by macroporous resin, and its physicochemical property and antioxidant activity were analyzed. The relevant research could establish the biological foundation for building a quantitative structure–function relationship and the application of purified BAP in functional food and medicine.

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Purification of flavonoids from Carex meyeriana Kunth based on AHP and RSM: Composition analysis, antioxidant, and antimicrobial activity

Cited by 31 publications

References 37 publications

Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products

Evaluation of the Antiwrinkle Activity of Enriched Isatidis Folium Extract and an HPLC–UV Method for the Quality Control of Its Cream Products

In Vitro and In Vivo Antioxidant Activity of Agave sisalana Agro-Industrial Residue

Composition analysis and antioxidant activity of purified Boletus auripes pigment using macroporous resin

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