Purification of galectin-3 from ovine placenta: Developmentally regulated expression and immunological relevance

Iglesias, M. Mercedes; Rabinovich, Gabriel A.; Ambrosio, Andrea L.; Castagna, Leonardo F.; Sotomayor, Claudia Elena; Wolfenstein‐Todel, Carlota

doi:10.1093/glycob/8.1.59

Cited by 26 publications

(23 citation statements)

References 29 publications

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“…4,42 On the other hand, redundancy is often built into mechanisms operating in key events during development as a form of fail-safe insurance. 43 Galectins have been implicated in several physiopathological processes requiring carbohydrate recognition, such as cell adhesion, 28 cell growth regulation, 44,45 immunomodulation, 46,47 apoptosis, 34,48,49 inflammation, 50 embryogenesis, 29 reproduction, 51,52 tumor spreading 53,54 and premRNA splicing. 35,55 The precise knowledge of the mechanism of action of galectins is lacking, although their effects are believed to relate to interactions with specific carbohydrate ligands.…”

mentioning

confidence: 99%

Galectins: an evolutionarily conserved family of animal lectins with multifunctional properties; a trip from the gene to clinical therapy

1999

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Galectins constitute a family of evolutionarily conserved animal lectins, which are defined by their affinity for poly-Nacetyllactosamine-enriched glycoconjugates and sequence similarities in the carbohydrate recognition domain. During the past decade, attempts to dissect the functional role for galectins in vivo have been unsuccessful in comparison to the overwhelming information reached at the biochemical and molecular levels. The present review deals with the latest advances in galectin research and is aimed at validating the functional significance of these carbohydrate-binding proteins. Novel implications of galectins in cell adhesion, cell growth regulation, immunomodulation, apoptosis, inflammation, embryogenesis, metastasis and pre-mRNA splicing will be particularly discussed in a trip from the gene to the clinical therapy. Elucidation of the molecular mechanisms involved in galectin functions will certainly open new avenues not only in biomedical research, but also at the level of disease diagnosis and clinical intervention, attempting to delineate new therapeutic strategies in autoimmune diseases, inflammatory processes, allergic reactions and tumor spreading.

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confidence: 99%

Galectins: an evolutionarily conserved family of animal lectins with multifunctional properties; a trip from the gene to clinical therapy

1999

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“…Future studies should challenge this paradigm in the context of other physiological systems. Consistently, expression of galectins-1 and -3 was found to be differentially regulated during mammalian gestation (43,44). Iglesias et al (44) reported the coexistence of mitogenic galectin-3 and apoptotic galectin-1 (43) in ovine placenta.…”

Section: Galectins-1 and -3: Why A Novel Paradigm?mentioning

confidence: 75%

“…Galectins have been involved in several in vitro physiopathological processes requiring carbohydrate recognition, such as cell adhesion (26), cell growth regulation (38), immunomodulation (39,40), apoptosis (32,41), inflammation (42), embryogenesis (28), reproduction (43,44), tumor spreading (45) and pre-mRNA splicing (25). It should be emphasized that the vast majority of galectin functions have been assigned to galectins-1 and -3.…”

Section: Functions Of Galectinsmentioning

confidence: 99%

“…That galectins could be implicated in the regulation of the immune response was first inferred from galectin-1 localization in immune privileged sites of the body, such as the placenta (6,43,44) and the eye (60) (61). Accordingly, galectin-1 could be proposed as an alternative regulatory signal to regulate immune privilege.…”

Section: Are Galectins Important In the Context Of Innate Immunity?mentioning

confidence: 99%

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Galectins: a key intersection between glycobiology and immunology

Rabinovich

Riera

Landa

et al. 1999

Braz J Med Biol Res

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Galectins are a family of evolutionarily conserved animal lectins, widely distributed from lower invertebrates to mammals. They share sequence and structure similarities in the carbohydrate recognition domain and specificity for polylactosamine-enriched glycoconjugates. In the last few years significant experimental data have been accumulated concerning their participation in different biological processes requiring carbohydrate recognition such as cell adhesion, cell growth regulation, inflammation, immunomodulation, apoptosis and metastasis. In the present review we will discuss some exciting questions and advances in galectin research, highlighting the significance of these proteins in immunological processes and their implications in biomedical research, disease diagnosis and clinical intervention. Designing novel therapeutic strategies based on carbohydrate recognition will provide answers for the treatment of autoimmune disorders, inflammatory processes, allergic reactions and tumor spreading.

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“…While galectin-1 has been shown to trigger T cell apoptosis (Perillo et a1.,1995;Rabinovich et al, 1998;Iglesias et al, 1998a), galectin-3 has been shown to stimulate proliferation (Yang et al, 1996;Iglesias et al, 1998b;Inohara et al, 1998). Similarly to members of the Bcl-2 family, galectins-1 and -3 belong to an additional family of proteins with high sequence homologies but opposite effects on cell survival.…”

Section: Galectin-1 Expression In Immune Privileged Sites" a Novel Mementioning

confidence: 99%

“Galectin‐1 Induces Central and Peripheral Cell Death:Implications in T‐Cell Physiopathology”

Sotomayor

Rabinovich

2000

Journal of Immunology Research

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The immune system has a remarkable capacity to maintain a state of equilibrium even as it responds to a diverse array of foreign proteins and despite its contact exposure to self-antigens. Apoptosis is one of the mechanisms aimed at preserving the homeostasis after the completion of an immune response, thus returning the immune system to a basal state and warranting the elimination of autoagressive cells in both central and peripheral lymphoid organs. Targeted deletions in critical genes involved in the apoptotic death machinery together with natural spontaneous mutations have clearly shown the importance of apoptosis in the regulation of the immune response. This complex scenario of stimulatory and inhibitory genes has been enriched with the finding that galectin-1, a 14.5 kDa [3-galactoside-binding protein, is able to induce apoptosis of immature cortical thymocytes and mature T cells by cross-linking cell surface glycoconjugates. Galectin-1 is present not only in central and peripheral lymphoid organs, but also at sites of immune privilege. In the present article we will discuss the implications of galectin-1-induced apoptosis in T-cell physiopathology in an attempt to validate its therapeutic potential in autoimmune and inflammatory diseases.Keywords: galectin-1, apoptosis, immunomodulation, macrophages, autoimmunity INTRODUCTION Death, along with growth and differentiation, is a critical part of the life cycle of the cell. Homeostasis control of cell number is thought to be the result of the dynamic balance between cell proliferation and cell death. It is only in the past ten years, that the attention has been focused on the physiological occurrence of cell death and its role in the homeostasis.Apoptosis or programmed cell death, is a phenomenon that plays a crucial role in a myriad of physiological and pathological processes. This review will briefly cover some relevant aspects of programmed cell death in the immune system in an attempt to provide valuable information about new molecules responsible for triggering death signals, such as galectin-1. The implications of this protein will be discussed in the context of T cell physiology and the regulation of central and peripheral tolerance. Finally, novel and intriguing findings will also be discussed implicating the use of this carbohydrate-binding protein in the treatment of autoimmune and inflammatory diseases.

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Purification of galectin-3 from ovine placenta: Developmentally regulated expression and immunological relevance

Cited by 26 publications

References 29 publications

Galectins: an evolutionarily conserved family of animal lectins with multifunctional properties; a trip from the gene to clinical therapy

Galectins: an evolutionarily conserved family of animal lectins with multifunctional properties; a trip from the gene to clinical therapy

Galectins: a key intersection between glycobiology and immunology

“Galectin‐1 Induces Central and Peripheral Cell Death:Implications in T‐Cell Physiopathology”

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