Purification of Gonadotrophin From Human Menopausal Urine

Donini, P.; Puzzuoli, D.; Montezemolo, R

doi:10.1530/acta.0.0450321

Cited by 38 publications

(17 citation statements)

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“…The Donini et al. (10), and Canfield et al (11) were tested in radioimmunoassays: (1) second international reference preparation of human postmenopausal gonadotropin (2IRPHMG) with biological activity of 40 international units (IU) per ampoule or 1 IU = 0.2295 mg for both hLH and human follicle-stimulating hormone (hFSH, follitropin); (2) (14). Cleavage from resin and removal of protecting groups were accomplished by treatment with liquid hydrogen fluoride.…”

mentioning

confidence: 99%

Evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin.

Chen¹,

Hodgen²,

Matsuura³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

167

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Substances from urinary extracts of normal, nonpregnant subjects and human pituitary gonadotropin preparations were found to react similarly to human chorionic gonadotropin (hCG) in a radioimmunoassay system that is highly specific for hCG and without crossreactivity to human luteinizing hormone (hLH). The COOH-terminus of the hCG,# subunit (6, 7). Unexpectedly, in radioimmunoassays using this antiserum and 125I-hCG, both the urinary extracts previously thought not to contain hCG and pituitary extracts were found to give rise to dose-response curves indistinguishable from those of highly purified hCG. These results were similar to those of the earlier assays (2). In order to elucidate the basis for these unexpected observations, radioimmunoassays were carried out prior to and following gel filtration of these extracts on Sephadex G-100. The results indicate that substances with immunological, physical, and biological properties of hCG are present in both crude and partially purified urinarygonadotropin preparations

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mentioning

confidence: 99%

Evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin.

Chen¹,

Hodgen²,

Matsuura³

et al. 1976

Proc. Natl. Acad. Sci. U.S.A.

167

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“…Esterification of the hydroxyl group of 17/3-hydroxyandrostano-[2,3-d]-isoxazole with 3-cyclohexylpropionic acid affords a potent anabolic agent with long duration of action and minimal androgenicity. A good myotrophic to androgenic ratio appears also to be present in 17/3-hydroxy-l7a-methylandrostano-[3,2-c]-isoxazole (Arnold & others, 1963b ;Donini & Montezemolo, 1961) where the side of the isoxazole ring involved in the ring fusion has been changed. This compound would seem to show negligible progestational activity and to have a low inhibitory effect on pituitary gonadotrophin production.…”

Section: Anabolic Agentsmentioning

confidence: 99%

Biological activity in steroids possessing nitrogen atoms: recent advances

Martin‐Smith

Sugrue

1964

Journal of Pharmacy and Pharmacology

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“…However, and contrary to the old and simple conception derived from animal experiments that FSH acts on the tubules and LH on Leydig cells [11], it was not until recent years that the availability of highly purified preparations of human pituitary (HPG) [10] and urinary preparations (HMG) [9] containing both FSH and LH activities, has made possible some progress of our knowledge in the regulation of human spermatogenesis. Although several earlier reports [2] have emphasized the usefulness of high and long-term administration of human chorionic gonadotropin (HCG) alone in stimulating germinal epithelium in prepuberal hypogonadotropic patients (eunuchoids) and, more recently, the necessity of adding prepara tions with FSH activity (HMG) for the completion of spermatogenesis [14, 28], no one was able to prove that the beneficial eifect of HCG could be replaced by testosterone, as it seems to occur in hypophysectomized rats [6].…”

Section: Introductionmentioning

confidence: 96%

Effect of Testosterone in the Recovery of Spermatogenesis in Hypophysectomized Patients

Mancini¹,

Lloret²,

Guitelman³

et al. 1971

Gynecol Obstet Invest

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A group of adult hypophysectomized patients (due to cromophobe adenoma) was studied at different periods of time after operation. All of them showed loss of libido and potentia, low levels of urinary FSH and LH and of testosterone glucoronide. Daily doses of corticoids and thyroid hormone were administered. Testis biopsies were taken before and after each one of the following courses of treatment. The 14 patients were divided into 5 different groups. Group I, comprising 3 patients, was treated first with HMG (Pergonal-500, 3 ampoules per week) combined with HCG (3,000 IU per week). After 16 weeks, HMG continued to be administered, but HCG was discontinued and replaced by testosterone propionate at different weekly doses in each patient (200, 100 or 50 mg) during another 16 weeks. The 3 patients of group II were first administered HMG (similar dose and period of time as above) and then with HMG combined with testosterone (200, 100 or 50 mg, respectively). The 3 patients of group III were first treated with HCG in similar dose and period of time as above, and then with HCG combined with testosterone in similar dose and period of time as above. The 3 patients of group IV were given testosterone alone in similar doses and period of time as above. Finally, the 2 patients of group V were used as controls and no gonadotropins or testosterone treatment were administered during the period of the research. The following was observed: (1) HMG combined with HCG induced restoration of full spermatogenesis, development of mature Leydig cells and regression of preexistent peritubular hyalinosis. HMG alone showed a partial recovery of Leydig cells and of spermatogenesis until spermatid stages. HCG induced development of germinal cells until middle meiosis process and complete development of Leydig cells. (2) When testosterone was added to HMG or to HCG, an inhibitory effect was noted as judged by the regression of spermatogenesis and Leydig cells development, which was accompanied by reappearance of peritubular hyalinosis. The intensity of this inhibitory effect was dose-dependent. When testosterone was given alone, an almost complete atrophy of seminiferous tubules and Leydig cells was present. (3) The probable mechanism of action of testosterone upon the endogenously circulating or exogenously administred gonadotropins is discussed.

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Purification of Gonadotrophin From Human Menopausal Urine

Cited by 38 publications

References 0 publications

Evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin.

Evidence for a gonadotropin from nonpregnant subjects that has physical, immunological, and biological similarities to human chorionic gonadotropin.

Biological activity in steroids possessing nitrogen atoms: recent advances

Effect of Testosterone in the Recovery of Spermatogenesis in Hypophysectomized Patients

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