Purification of high value proteins from particle containing potato fruit juice via direct capture membrane adsorption chromatography

Schoenbeck, I.; Graf, Alain-Michel; Leuthold, Martin; Pastor, Agustı́n; Beutel, Sascha; Scheper, Thomas

doi:10.1016/j.jbiotec.2013.09.018

Cited by 29 publications

(16 citation statements)

References 15 publications

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“…; Schoenbeck et al . ). At present, potato fruit juice is treated and utilized as fertilizer or animal feed (Andersson et al .…”

Section: Introductionmentioning

confidence: 97%

In Silico Assessment of the Potential of Patatin as a Precursor of Bioactive Peptides

Zhu

et al. 2015

Journal of Food Biochemistry

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The aim of this study was to evaluate patatin, a major storage protein in potato tuber, as a precursor of bioactive peptides based on in silico approaches. In silico proteolysis of patatin using 16 proteases released numerous bioactive peptides exhibiting antioxidant, angiotensin-I converting enzyme (ACE) inhibitory, dipeptidyl peptidase (DPP)-IV inhibitory, glucose uptake, neuropeptide inhibitory and ion flow-regulating capacities. Chymotrypsin C released the most bioactive sequences from patatin compared with other proteases. In addition, the results of PeptideRanker demonstrated that a number of di-and tripeptides derived from patatin possessed great potential as bioactive peptides. PRACTICAL APPLICATIONSPatatin is a major protein in potato fruit juice, which is regarded as a by-product of potato starch industry. Effective conversion of patatin into high value-added ingredients will significantly improve its potential for food application and elevate its commercial value. This study provides a theoretical basis for development of patatin as a high value-added and biofunctional ingredient in the food industry.

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“…; Schoenbeck et al . ). At present, potato fruit juice is treated and utilized as fertilizer or animal feed (Andersson et al .…”

Section: Introductionmentioning

confidence: 97%

In Silico Assessment of the Potential of Patatin as a Precursor of Bioactive Peptides

Zhu

et al. 2015

Journal of Food Biochemistry

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“…However, the low flux and short lifespan of UF method may impede its wide industrial application. In contrast, direct capture membrane adsorption chromatography has been developed to bind native potato proteins instead of retention (Schoenbeck et al ., ), which can permit high flow and more loading of PFJ as well as remove phenolic compounds. Furthermore, this method is extremely effective to recover patatin using quaternary ammonium (Q‐membrane) with long‐term stability.…”

Section: Separation and Purification Of Patatinmentioning

confidence: 99%

“…Potato is the world's fourth most important food crop after rice, wheat and corn, while China is the world's largest potato producing country with around 87 million metric tons each year (Elahi & Mu, ; Zhang et al ., ). During industrial potato starch manufacturing, a dilute by‐product known as potato fruit juice (PFJ) is produced (Løkra et al ., ; Schoenbeck et al ., ). On a dry matter basis, PFJ contains approximately 2% (w/w) protein, which has great potential as a high value‐added food ingredient (Barta et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Towards potato protein utilisation: insights into separation, functionality and bioactivity of patatin

Liu

Soladoye

2019

Int J of Food Sci Tech

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Potato fruit juice (PFJ) is a dilute industrial by-product of potato starch industry containing potato protein that is abundant in patatin, a major storage protein. From an economic standpoint, conversion of potato protein into a high value-added food ingredient has received considerable attention. In this review, the structural and physicochemical characteristics of patatin are introduced. The separation and purification methods of patatin are compared and summarised. The functional properties of patatin as food ingredients are comprehensively reviewed. The beneficial effects of patatin on health and food application are emphatically presented. In addition, the current challenges for development of patatin as a functional food ingredient are also discussed. This review offers a theoretical basis for further application of patain in the food industry. Functionality and bioactivity of patatin Y. Fu et al. 2315 Functionality and bioactivity of patatin Y. Fu et al.

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“…Therefore, membrane chromatography with tangential flow has been recently investigated by several research groups. In food industry applications, it has been implemented for the recovery of bioactive proteins from industrial potato fruit juice [5,6] or for the separation of glycosylated caseinomacropeptide from skim milk whey [7]. In the area of recombinant technology, Orr et al used the integration of tangential flow and membrane chromatography to obtain a one-step purification of an extracellularly produced enzyme [8].…”

Section: Introductionmentioning

confidence: 99%

Considerations on the flow configuration of membrane chromatography devices for the purification of human basic fibroblast growth factor from crude lysates

Gieseler

Pepelanova

Meyer

et al. 2016

Engineering in Life Sciences

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Membrane chromatography possesses numerous advantages such as operation at high flow rates, low back pressure, ease of handling and scale up, which make the membrane adsorber process a viable alternative to conventional packed column chromatography. A purification process for the isolation of human recombinant basic fibroblast growth factor (FGF‐2) based on membrane chromatography was investigated using devices with different flow configurations. In the first process, the FGF‐2 capture step was performed with an axial flow device, while the alternative method achieved direct capture of FGF‐2 from unclarified cell lysate with a tangential flow device. In both processes, FGF‐2 purities exceeded 82% and the purified cytokine displayed high biological activity. Binding capacity (BC) from fermentation broth of the axial flow device was 28 mg/mL. This was 50% higher than the BC obtained with the tangential flow device under particle‐free supernatant conditions (18 mg/mL) and 150% higher compared to the BC achieved with unclarified cell lysate (11 mg/mL). While membrane chromatography in tangential flow mode omits clarification and thus reduces the number of stages in the downstream process, it displays lower peak resolution and leads to a lower overall process yield.

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Purification of high value proteins from particle containing potato fruit juice via direct capture membrane adsorption chromatography

Cited by 29 publications

References 15 publications

In Silico Assessment of the Potential of Patatin as a Precursor of Bioactive Peptides

In Silico Assessment of the Potential of Patatin as a Precursor of Bioactive Peptides

Towards potato protein utilisation: insights into separation, functionality and bioactivity of patatin

Considerations on the flow configuration of membrane chromatography devices for the purification of human basic fibroblast growth factor from crude lysates

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