Purification of human immunoglobulin G: a new approach to plasma fractionation

Abstract

The technology has been shown to be linearly scalable and has the capacity to contribute to increased production of important plasma fraction therapeutics.

Evaluation of commercial chromatographic adsorbents for the direct capture of polyclonal rabbit antibodies from clarified antiserum

“…Currently the large-scale purification of polyclonal antibodies is, for the most part, carried out by the plasma fractionation industry, with production being geared towards generation of a wide range of plasma proteins (including albumin, Factor VIII, Factor IX, Protein C and von Willebrand Factor) rather than single antibody products [1][2][3]. Since the first reports on the use of immobilised Protein A for the affinity purification of antibodies over 30 years ago [4,5].…”

“…Since the first reports on the use of immobilised Protein A for the affinity purification of antibodies over 30 years ago [4,5]. Protein A affinity chromatography has become the industrial standard for the purification of clinical grade monoclonal antibodies [3,[6][7][8], but is rarely applied in the plasma fractionation industry [1,2], largely for reasons of cost and reservations concerning Protein A's chemical stability, adsorbent shelf-life and cleaning [9]. For the production of diagnostic antibody products such concerns are less serious; thus Protein A chromatography currently represents one of most potent separation tools available to the diagnostic industry.…”

Poly(2-dimethylamino ethylmethacrylate)-Based Polymers To Camouflage Red Blood Cell Antigens

“…Immediately after, the cell suspension was incubated under lateral agitation (CAT model M5, CAT Ingenieurburo, Staufen, DE) at a rate of 36 rpm for 1 h at 37°C. This second mixing was performed at room temperature (RT°; 25°C) for the homopolymers, whereas for the copolymers, three distinct temperatures (25,30, and 37°C) have been investigated. After that, 1 mL of PBS was added to the treated RBCs to wash and centrifuge them (Mini Spin Plus Microcentrifuge, Eppendorf) for 10 min at 600 rpm.…”

Label-free capacitive immunosensors for ultra-trace detection based on the increase of immobilized antibodies on silver nanoparticles

“…The electrodes prepared with 48 pmol of nanoparticles provided the highest immobilized yield 88.2-88.6% (3.5 × 10 13 molecules, calculated using a molecular weight of 150 kDa for an IgG [28,29]). This agreed well with the larger surface area as indicated by the higher anodic peak current.…”