Purification of hydrophobic complex antibody formats using a moderately hydrophobic mixed mode cation exchange resin

Koehnlein, Wolfgang; Holzgreve, Annika; Schwendner, Klaus; Skudas, Romas; Schelter, Florian

doi:10.1016/j.chroma.2022.463696

Journal of Chromatography A

2023

DOI: 10.1016/j.chroma.2022.463696

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Purification of hydrophobic complex antibody formats using a moderately hydrophobic mixed mode cation exchange resin

Wolfgang Koehnlein

Annika Holzgreve

Klaus Schwendner

et al.

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2024

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Cited by 11 publications

References 18 publications

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Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Goodarzi,

Jalalirad

2024

Biotech & Bioengineering

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Increasing attention has been paid to the purity of therapeutic proteins imposing extensive costs and challenges to the downstream processing of biopharmaceuticals. One of the efforts, that has been exerted to overcome such limitations, was developing multimodal or mixed‐mode chromatography (MMC) resins for launching selective, orthogonal, non‐affinity purification platforms. Despite relatively extensive usage of MMC resins, their real potential and fulfillment have not been extensively reviewed yet. In this work, the explanation of practical and key aspects of downstream processing of recombinant proteins with or without MMC resins was debated, as being useful for further purification process development. This review has been written as a step‐by‐step guide to deconvolute both inherent protein purification and MMC complexities. Here, after complete elucidation of the potential of MMC resins, the effects of frequently used additives (mobile phase modifiers) and their possible interactions during the purification process, the critical characteristics of common product‐related impurities (e.g., aggregates, charge variants, fragments), host‐related impurities (e.g., host cell protein and DNA) and process related impurities (e.g., endotoxin, and viruses) with solved or unsolved challenges of traditional and MMC resins have been discussed. Such collective experiences which are reported in this study could be considered as an applied guide for developing successful downstream processing in challenging conditions by providing a clear insight into complex MMC resins and impurities.

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Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Goodarzi,

Jalalirad

2024

Biotech & Bioengineering

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General strategies for IgG‐like bispecific antibody purification

2024

Biotechnology Progress

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Bispecific antibodies (bsAbs) can simultaneously bind two different antigens or epitopes. Their dual‐targeting capability enables novel mechanisms of action, gaining therapeutic advantages over conventional monospecific mAbs. In recent years, the number of bsAbs grows rapidly and bsAbs under development are available in diverse formats. In particular, Fc‐containing IgG‐like bsAbs, which represent the major group, can be constructed in asymmetric or symmetric format. For asymmetric ones, whose assembly requires multiple distinct chains, although numerous strategies have been developed to promote desired chain pairing, product‐related variants such as free chains, half molecules and mispaired species are usually present at various levels. For symmetric ones, increased level of aggregates and truncating variants is often associated with their production. In general, bsAbs pose greater challenges to the downstream team than regular mAbs. In the past few years, our team successfully developed the downstream process for over 70 bsAbs in greater than 30 different formats and accumulated substantial experience. This review introduces general strategies that we have used while purifying these challenging molecules.

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Progress of ligand-modified agarose microspheres for protein isolation and purification

Qi,

Chen

2024

Microchim Acta

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Purification of hydrophobic complex antibody formats using a moderately hydrophobic mixed mode cation exchange resin

Cited by 11 publications

References 18 publications

Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

Clear insight into complex multimodal resins and impurities to overcome recombinant protein purification challenges: A review

General strategies for IgG‐like bispecific antibody purification

Progress of ligand-modified agarose microspheres for protein isolation and purification

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