Purification of kidney alkaline phosphatase

Alvarez, E. Fernández; Lora‐Tamayo, M.

doi:10.1042/bj0690312

Cited by 16 publications

(2 citation statements)

References 20 publications

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“…The partially purified enzyme [6] and the kidney tissue homogenate showed similar alkaline phosphatase kinetic behaviours. A first-order rate of reaction was obtained with 6-11 mmol, 2-glycerophosphate ( fig.…”

Section: Discussionmentioning

confidence: 80%

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Identity of Rat Kidney and Urine Alkaline Phosphatase Actions and Relation to the Elfect of Nitrofuranfuradroxyl

Awad¹,

Nf²

1975

Enzyme

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The activities of alkaline phosphatases of kidney and urine appear similar and identical in action. Both are magnesium-dependent and inhibited by higher concentrations of 2-glycerophosphate. A band of similar mobility is obtained on electrophoresis of kidney and urine enzymes. In vitro magnesium competitively inhibits their activities when the ratio of Mg^+2 ions/ 2-glycerophosphate is above a definite level. Their activities are not affected by exogenous zinc and follow the Michaelis-Menten equation only when attention is given to the ratio of Mg^+2ions/substrate. Nitrofuranfuradroxyl is a mixed type inhibitor showing a second-order rate of reaction with kidney and urine phosphatases.

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Section: Discussionmentioning

confidence: 80%

“…The activity of alkaline phosphatase was estimated in partially purified [6] enzyme and in tissue homogenate in different concentrations (30-70 mg of tissue/ml). Mg+2 (0.3-3.2 mmol/ml) and 2-glycerophosphate (5.8-23.3 mmol/1) were used.…”

Section: Methodsmentioning

confidence: 99%

Identity of Rat Kidney and Urine Alkaline Phosphatase Actions and Relation to the Elfect of Nitrofuranfuradroxyl

Awad¹,

Nf²

1975

Enzyme

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SÄuenchromatographie Von Enzymen

Turba¹

1960

Advances in Enzymology - And Related Areas of Molecular Biology

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Untersuchungen über Esterasen: Charakterisierung der aktiven Gruppen der sauren Phosphormonoesterase der Kartoffel

Lora‐Tamayo¹,

Alvarez²

1959

Fette, Seifen, Anstrichm.

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kaltem absol. Chloroform. AnscblieBend wird im Vakuumexsikkator getrocknet. Ausbeute 81 O / o , Schmp. 244"-245' C. rz H2 I 111. Synthese der Sterin-und Tritcrprn-betairiester nach P. Plattner und M . Geiger18 1 g des Sterins oder Triterpens wird mit 3 g Saurechlorid des Betains und 10 ml absol. Chloroform unter sorgfaltigem Ausschlui3 von Feuchtigkeit 2 Std. zum Sieden erhitzt. Gegen Ende der Reaktion fugt man noch 5 ml Chloroform hinzu. Die erkaltete Chloroform-Losung engt man nach dem Abfiltrieren des iiberschussigen Saurechlorids im Vakunm ein. Der Destillationsruckstand wird im Soxhlet 6 Std. mit trockenem Ather, der die Sterin-und Triterpen-betainester nicht lost, zwecks Entfernens von Ausgangsmaterial extrahiert. Die so gereinigten Sterin-und Triterpen-betainester werden aus absol. Methanol bzw. aus Aceton-Ather umkristallisiert. 18Helv. chim. Acta 28, 1362 [1945]. a-Amyryl-betainestcr-chlorid C,,H,,OINCl ber.: C 74.70°/o H 10.65 O / o N 2.49 O / o gef.: C 73.00 O / o H 10.90 "/a N 2.72 "/a Die Betainester der Ursolund Oleanolsaure sind so stark hygroskopisch, d a 8 sie nicht direkt, sondern nur als Pikratc fur die Analyse Anwendung finden konnten. Oleanolsaure-betainester-chlorid-pikrat, Schmp. 169"-176' C C,lHe;~OllN,CI ber.: C 62.80 O/o H 7.66O/o N 7.13 "/o gef.: C 60.45 O/o

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Purification of kidney alkaline phosphatase

Cited by 16 publications

References 20 publications

Identity of Rat Kidney and Urine Alkaline Phosphatase Actions and Relation to the Elfect of Nitrofuranfuradroxyl

Identity of Rat Kidney and Urine Alkaline Phosphatase Actions and Relation to the Elfect of Nitrofuranfuradroxyl

SÄuenchromatographie Von Enzymen

Untersuchungen über Esterasen: Charakterisierung der aktiven Gruppen der sauren Phosphormonoesterase der Kartoffel

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