Purification of lactoferrin from sweet whey using ultrafiltration followed by expanded bed chromatography

Maciel, Kátia Silva; Santos, Leandro Soares; Bonomo, Renata Cristina Ferreira; Veríssimo, Lizzy Ayra Alcântara; Minim, Valéria Paula Rodrigues; Minim, Luís Antônio

doi:10.1016/j.seppur.2020.117324

Cited by 18 publications

(20 citation statements)

References 31 publications

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“…The source and manufacturing process greatly affect the composition and functionality of whey proteins, which can reach approximately 80% purity. Whey sub-fractions such as lactoferrin pose manufacturing and purification challenges [ 146 ]. Whey proteins have a high nutritional value and demonstrate antimicrobial, antioxidant, and anticancer properties [ 36 , 147 ].…”

Section: Electrospinning Animal-derived Proteins For Wound Healingmentioning

confidence: 99%

“…Lactoferrin is a globular glycoprotein with nutritional, antimicrobial, anti-inflammatory and anti-oxidant properties [ 146 ]. Due to its iron-chelating property, it exerts a wide range of bacteriostatic effects on a variety of pathogens [ 96 , 97 ], including Gram-positive and Gram-negative bacteria, viruses and fungi [ 153 , 154 ].…”

Section: Electrospinning Animal-derived Proteins For Wound Healingmentioning

confidence: 99%

“…Proteins such as soy protein [ 83 ], keratin, collagen, gelatin and elastin, for example, carry RGD or other cell-recognition motifs that facilitate recognition by cells, cell adhesion to fibers and cell migration across the wound bed ( Figure 1 ). Proteins such as soy protein [ 88 ], lactoferrin [ 146 ] and lysozyme [ 98 ] further possess innate antimicrobial, anti-oxidant and anti-inflammatory activity that facilitate healing. The biological properties and further beneficial features of proteins of plant and animal origin that are used for electrospinning in the wound healing context are summarized in Table 4 .…”

Section: Opportunities and Challenges Of Using Proteins For Electrmentioning

confidence: 99%

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Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Akhmetova

Heinz

2020

Pharmaceutics

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With the growth of the aging population worldwide, chronic wounds represent an increasing burden to healthcare systems. Wound healing is complex and not only affected by the patient’s physiological conditions, but also by bacterial infections and inflammation, which delay wound closure and re-epithelialization. In recent years, there has been a growing interest for electrospun polymeric wound dressings with fiber diameters in the nano- and micrometer range. Such wound dressings display a number of properties, which support and accelerate wound healing. For instance, they provide physical and mechanical protection, exhibit a high surface area, allow gas exchange, are cytocompatible and biodegradable, resemble the structure of the native extracellular matrix, and deliver antibacterial agents locally into the wound. This review paper gives an overview on cytocompatible and biodegradable fibrous wound dressings obtained by electrospinning proteins and peptides of animal and plant origin in recent years. Focus is placed on the requirements for the fabrication of such drug delivery systems by electrospinning as well as their wound healing properties and therapeutic potential. Moreover, the incorporation of antimicrobial agents into the fibers or their attachment onto the fiber surface as well as their antimicrobial activity are discussed.

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Section: Electrospinning Animal-derived Proteins For Wound Healingmentioning

confidence: 99%

Section: Electrospinning Animal-derived Proteins For Wound Healingmentioning

confidence: 99%

Section: Opportunities and Challenges Of Using Proteins For Electrmentioning

confidence: 99%

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Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Akhmetova

Heinz

2020

Pharmaceutics

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“…The use of expanded beds allows a better homogenization of cell culture media. Methodologies such as adsorption or fluidized bed chromatography are currently being established [2,3], which have had promising results in the extraction of biological products of industrial interest, given the reduction of the operating volume, which is the objective of process intensification [4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Fluidized State Stability From Radioactive Particle Tracking Results Applied

Salierno¹,

Gradišek²,

Maestri³

et al. 2021

Preprint

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Currently, various industrial processes are carried out in fluidized bed reactors, such as fermentation, water treatment, and algae growth. Knowing its internal dynamics is fundamental for the intensification of these processes. This work assesses the motion of fluidized calcium alginate spheres under the influence of an upward fluid flow within a 1.2 m high and 0.1 m inner diameter acrylic column. The Radioactive Particle Tracking technique is a proper methodology to study the internal dynamics of these kinds of equipment. Liquid-Solid Fluidized Bed is compared with the Gas-Liquid-Solid Fluidized Bed operation mode in terms of mixing behavior. Data gathered is analyzed in view of Shannon entropy as a dynamic mixing measure. Mixing times are found to be of the same order of magnitude. However, the Liquid-Solid Fluidized Bed achieves a lower homogenization degree. Also, we found good agreement between two criteria for assessing stability for the mixed state: the Shannon Entropy time-series plateau and the Glansdorff-Prigogine criterion based on Fisher information.

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“…The use of expanded beds allows for better homogenization of the cell culture media. Methodologies such as adsorption or fluidized bed chromatography are currently being established [5,6], which have had promising results in the extraction of biological products of industrial interest, given the reduction in the operating volume [7][8][9]. Their underlying dynamics are fundamental for properly designing the units and implementing process intensification strategies [10,11].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Fluidized State Stability from Radioactive Particle Tracking Results

et al. 2021

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Currently, various industrial processes are carried out in fluidized bed reactors. Knowing its internal dynamics is fundamental for the intensification of these processes. This work assesses the motion of fluidized calcium alginate spheres under the influence of an upward fluid flow within a 1.2 m high and 0.1 m inner diameter acrylic column. The liquid–solid fluidized bed was compared with a gas–liquid–solid fluidized bed operation mode in terms of mixing behavior. The radioactive particle tracking technique is a proper methodology to study the internal dynamics of these kinds of equipment. Data gathered were analyzed with Shannon entropy as a dynamic mixing measure. Mixing times were found to be between 1 and 2.5 seconds for both fluidization modes. The liquid–solid fluidized bed presents a rather smooth mixing time profile along the column. On the other hand, the gas–liquid–solid fluidized bed showed high sensitivity of entropy production with height, reaching a sharp tendency break at the second quartile of the column. The Glansdorff–Prigogine stability measure can accurately capture flow regime transitions of the gas–liquid–solid fluidized bed, allowing it to be used to construct reliable operative windows for fluidization equipment.

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Purification of lactoferrin from sweet whey using ultrafiltration followed by expanded bed chromatography

Cited by 18 publications

References 31 publications

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Electrospinning Proteins for Wound Healing Purposes: Opportunities and Challenges

Comparison of the Fluidized State Stability From Radioactive Particle Tracking Results Applied

Comparison of the Fluidized State Stability from Radioactive Particle Tracking Results

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