Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption

Niu, Jianfeng; Chen, Zhangfan; Wang, Guangce; Zhou, Biao

doi:10.1007/s10811-009-9420-2

Cited by 39 publications

(26 citation statements)

References 31 publications

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“…A28DM "Ethodin" precipitation; Gel filtration chromatography 3.2 66.0 PE Niu et al [82] Porphyra yezoensis Expanded bed absorption; Ion-exchange chromatography > 4 -APC Su et al [83] S. platensis Ammonum sulphate precipitation; Hydroxyapatite chromatography; Ion-exchange chromatography 5 43.0 PE Parmar et al [84] Phormidium sp. A27DM, Lyngbya sp.…”

Section: Pc and Apcmentioning

confidence: 99%

Recent advances in production, purification and applications of phycobiliproteins

Sonani

Rastogi

Patel

et al. 2016

WJBC

159

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light-harvesting, PBPs are found to involve in several life sustaining phenomena including photoprotection in cyanobacteria. The unique spectral features (like strong absorbance and fluorescence), proteineous nature and, some imperative properties like hepato-protective, antioxidants, anti-inflammatory and anti-aging activity of PBPs enable their use in food, cosmetics, pharmaceutical and biomedical industries. PBPs have been also noted to show beneficial effect in therapeutics of some disease like Alzheimer and cancer. Such large range of applications increases the demand of PBPs in commodity market. Therefore, the large-scale and coast effective production of PBPs is the real need of time. To fulfil this need, many researchers have been working to find the potential producer of PBPs for the production and purification of PBPs. Results of these efforts have caused the inventions of some novel techniques like mixotrophic and heterotrophic strategies for production and aqueous two phase separation for purification purpose. Overall, the present review summarises the recent findings and identifies gaps in the field of production, purification and applications of this biological and economically important proteins. Core tip: The present review compiles the overall research that has been done so far in the field of produ ction, purification and application of phycobiliptoteins (PBPs). The comparative accounts for different methods for PBPs production, purification have been discussed. The potential applications of PBPs in various industries including food, cosmetics and pharmaceutical have been debated rationally. Remaining questions and rese arch gaps in respected field have been highlighted as conclusion. Recent advances in production, purification and applications of phycobiliproteins AbstractAn obligatory sunlight requirement for photosynthesis has exposed cyanobacteria to different quantity and quality of light. Cyanobacteria can exhibit efficient photosynthesis over broad region (450 to 650 nm) of solar spectrum with the help of brilliantly coloured pigment proteins called phycobiliproteins (PBPs). Besides REVIEW 100February 26, 2016|Volume 7|Issue 1|

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Section: Pc and Apcmentioning

confidence: 99%

Recent advances in production, purification and applications of phycobiliproteins

Sonani

Rastogi

Patel

et al. 2016

WJBC

159

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“…For example, 28 g P. yezoensis was smashed and applied to phenyl-sepharose column to obtain 27 mg PE with a purity of 2.0 -2.5. After purification with another DEAE-sepharose column, 23 mg PE with a purity of 4.5 was obtained [23]. In this study, we applied 400 g P. yezoensis and extracted 1292 mg PE and 592 PC with purity above 3.2 after one time of chromatography.…”

Section: Large Scale Preparation Of Pbp From P Yezoensismentioning

confidence: 99%

“…It has been reported that PE from P. yezoensis has two peaks at 498 nm and 565 nm in absorption spectrum [23] and its characteristic fluorescence emission peak is at 576 nm. The peak of absorption spectrum of PC is at 615 nm [35] and the fluorescence emission peak is between 630 nm and 642 nm [36].…”

Section: Large Scale Preparation Of Pbp From P Yezoensismentioning

confidence: 99%

“…In addition, in routine process the quality of products varies according with raw materials [22]. For example, hydrophobic chromatography was proved to be unsuitable for the purification of PBP from P. yezoensis [23]. Furthermore, routine methods have bottlenecks, e.g., high cost, long cycle and low yield in column chromatographic purification step.…”

Section: Introductionmentioning

confidence: 99%

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Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate

Cai¹,

Li²,

Wu³

et al. 2012

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The purpose of this study was to extract phycobiliproteins (PBP) from Porphyra yezoensis using an optimized procedure and further establish a large scale process for protein production. According to our previous experiences on the extraction of PBP, salting out methods, e.g. ammonium sulfate precipitation, worked more efficiently than isoelectric precipitation, differential centrifugation or ultrafiltration. Thus, we chose ammonium sulfate to coprecipitate PBP in crude solution. After four times of precipitation followed by one time of high speed centrifugation, the maximum purity of crude phycoerythrin and phycocyanin reached 1.94 (A 565 /A 280 ) and 0.85 (A 615 /A 280 ), with a yield of 0.50% and 0.37%, respectively. A total of 0.94 mg phycoerythrin and 0.54 mg phycocyanin with purity of more than 3.2 were obtained from 1 g dried P. yezoensis after additional chromatography. We further scaled up the frozen dried P. yezoensis from 20 g to 400 g, with 1295 mg phycoerythrin and 593 mg phycocyanin obtained.

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“…ECS-18(Kim et al 2011). However, unlike PE isolated form Porphyra yezoensis(Niu et al 2010) and Grateloupia PE promotes paraquat resistance and reduces Aβ aggregationdependent paralysis. a Time course of temperature-induced paralysis in C. elegans strain CL4176 and mean paralysis time (inset) in four different conditions.…”

mentioning

confidence: 95%

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

Sonani

Rastogi

Singh³

et al. 2016

Protoplasma

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In vitro antioxidant virtue and life-prolonging effect of phycoerythrin (PE; a pigment protein isolated from Phormidium sp. A09DM) have been revealed in our previous reports (Sonani et al. in Age 36:9717, 2014a; Sonani et al. in Process Biochem 49:1757-1766, 2014b). It has been hypothesized that the PE expands life span of Caenorhabditis elegans (bears large resemblance with human aging pathways) due to its antioxidant virtue. This hypothesis is tested in present study by checking the effect of PE on intracellular reactive oxygen species (ROS) generation and associated physiological deformities using mouse and human skin fibroblasts, C. elegans, and Drosophila melanogaster Oregon R and by divulging PE's structural attributes responsible for its antioxidant asset. PE treatment displayed noteworthy decrease of 67, 48, and 77 % in ROS level in mouse fibroblast (3T3-L1), human fibroblast, and C. elegans N2, respectively, arisen under chemical-induced oxidative stress. PE treatment delayed the development of paraquat-induced Alzheimer phenotype by 14.5 % in C. elegans CL4176. Furthermore, PE improved the locomotion of D. melanogaster Oregon R under oxidative stress with simultaneous up-regulation in super-oxide dismutase and catalase activities. The existence of 52 Glu + Asp + His + Thr residues (having metal ion sequestration capacity), 5 phycoerythrobilin chromophores (potential electron exchangers) in PE's primary structure, and significant hydrophobic patches on the surface of its α- and β-subunits are supposed to collectively contribute in the antioxidant virtues of PE. Altogether, results support the hypothesis that it is the PE's antioxidant asset, which is responsible for its life-prolonging effect and thus could be exploited in the therapeutics of ROS-associated abnormalities including aging and neurodegeneration in eukaryotes.

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Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption

Cited by 39 publications

References 31 publications

Recent advances in production, purification and applications of phycobiliproteins

Recent advances in production, purification and applications of phycobiliproteins

Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

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Purification of phycoerythrin from Porphyra yezoensis Ueda (Bangiales, Rhodophyta) using expanded bed absorption

Cited by 39 publications

References 31 publications

Recent advances in production, purification and applications of phycobiliproteins

Recent advances in production, purification and applications of phycobiliproteins

Large scale preparation of phycobiliproteins from &lt;i&gt;Porphyra yezoensis&lt;/i&gt; using co-precipitation with ammonium sulfate

Phycoerythrin averts intracellular ROS generation and physiological functional decline in eukaryotes under oxidative stress

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Large scale preparation of phycobiliproteins from <i>Porphyra yezoensis</i> using co-precipitation with ammonium sulfate