Purification of Puerarin from Pueraria lobata by FCPC versus HSCCC Using Small-volume Columns

Sun, Bo; Yang, Ye; Hu, Xin; Xie, Xin-xin; Meucci, Jérémy; Fang, Ming; Zhao, Lijuan

doi:10.1016/s1674-6384(14)60022-8

Cited by 6 publications

(6 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The optimal extraction conditions were found to be: 50% ethanol (as extractant), 650 W ultrasonic power at 298.15 K with agitation rate at 300 rpm. Sun et al [115] replaced the solvent by 40% ethanol for extracting puerarin from this plant material. The usage of an ethanol-water mixture for UAE also led to the successful quantification of puerarin, daidzin, daidzein, and genistein in the roots of Pueraria lobata and P. thomsonii [116].…”

Section: Green Extraction Techniquesmentioning

confidence: 99%

Selectivity of Current Extraction Techniques for Flavonoids from Plant Materials

et al. 2020

View full text Add to dashboard Cite

Flavonoids have a broad spectrum of established positive effects on human and animal health. They find an application in medicine for disease therapy and chemoprevention, whence the interest in flavonoids increases. In addition, they are used in food and cosmetic industries as pigments and biopreservatives. Plants are an inexhaustible source of flavonoids. The most important step of plant raw material processing is extraction and isolation of target compounds. The quality of an extract and efficiency of a procedure are influenced by several factors: Plant material and pre-extracting sample preparation, type of solvent, extraction technique, physicochemical conditions, etc. The present overview discusses the common problems and key challenges of the extraction procedures and the different mechanisms for selective extraction of flavonoids from different plant sources. In summary, there is no universal extraction method and each optimized procedure is individual for the respective plants. For an extraction technique to be selective, it must combine an optimal solvent or mixture of solvents with an appropriate technique. Last but not least, its optimization is important for a variety of applications. Moreover, when the selected method needs to be standardized, it must achieve acceptable degree of repeatability and reproducibility.

show abstract

Section: Green Extraction Techniquesmentioning

confidence: 99%

Selectivity of Current Extraction Techniques for Flavonoids from Plant Materials

et al. 2020

View full text Add to dashboard Cite

show abstract

“…The suitable K D values (in the range of 0.5–2.0) for counter-current chromatography were established by Ito [ 38 ]. Referring to the successful results of separation and purification of puerarin by FCPC in the ethyl acetate- n -butanol-water system published by Sun et al [ 31 ], we first decided to test this solvent composition using different volume ratios of the individual components ( Table 1 ).…”

Section: Resultsmentioning

confidence: 99%

“…Further improvements to the method were made by Li et al [ 19 ] who obtained daidzein and its four glycosides (daidzin, puerarin, 3′-hydroxypuerarin, and 3′-methoxypuerarin) using HPCCC and n -hexane-ethyl acetate- n -butanol-ethanol-water (0.5:2:1:0.5:3.5, V / V / V / V / V ) as a biphasic solvent system. To date, there has been only one scientific report, published by Sun et al [ 31 ], on the use of fast centrifugal partition chromatography (FCPC) for the micropreparative isolation of the predominant compound (puerarin) of a dry extract of P. lobata using a mobile/stationary phase system consisting of ethyl acetate- n -butanol-water in a 2:1:3 ( V/V/V ) ratio. The researchers also confirmed the superiority of FCPC over the comparatively used HSCCC in the one-step isolation and purification of puerarin, resulting in better resolution of the other components of the kudzu extract and higher purity of the aforementioned isoflavone glucoside.…”

Section: Introductionmentioning

confidence: 99%

Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques

2022

View full text Add to dashboard Cite

Pueraria lobata (Willd.) Ohwi is a legume taxon native to Southeast Asia and widely used in traditional medicine systems of that region. The therapeutic applications of the underground parts of this species (known as kudzu root) are related to its high content of isoflavones, mainly the characteristic C-glycoside derivatives. Within this group, the most explored compound both phytochemically and pharmacologically is puerarin. However, current scientific findings document important anti-biodegenerative effects for some of the minor isoflavones from kudzu roots. Therefore, the main objective of the study was to develop an original preparative method that allowed the efficient isolation of closely related hydrophilic daidzein C-glycosides, including mirificin, from vacuum-dried aqueous-ethanolic extracts of kudzu roots. For this purpose, the combined centrifugal partition (CPC) and flash chromatographic (FC) techniques were elaborated and used. The optimized biphasic solvent system in CPC, with ethyl acetate, ethanol, water, and 0.5% (V/V) acetic acid as a mobile phase modifier, enabled the purification and separation of the polar fraction containing bioactive isoflavones and ultimately the isolation of mirificin, 3′-hydroxy- and 3′-methoxypuerarin, puerarin, and daidzin using FC. The identity of isoflavones was confirmed using spectroscopic (UV absorption and nuclear magnetic resonance) and mass spectrometric methods. The determined purity of isolated mirificin was 63%.

show abstract

“…In general, these purification methods require several steps with high solvent consumption, and they are usually very time‐consuming methodologies with lower recovery of the natural product. Alternatively, other techniques have been applied to the isolation and purification of natural products that solve almost all the limitations of the traditional purification methods [43,44] . One of these techniques is the countercurrent chromatography (CCC), which is a liquid‐liquid partition chromatography that does not have a solid stationary phase support.…”

Section: Resultsmentioning

confidence: 99%

Recovery and Seasonal Variation of Cinnamtannin B‐1 from Laurel (Laurus nobilis L.) Pruning Wood Wastes

Alejo-Armijo

Ortega-Vidal

Salido

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

Cinnamtannin B‐1 (C‐B1) is a commercially‐available trimeric A‐type procyanidin with remarkable cellular actions mainly derived from its high radical scavenging activity. C‐B1 is the main phenolic compound of laurel wood, which has previously been isolated by a combination of conventional chromatographic techniques. The first aim of this work was to find laurel trees containing as much C‐B1 as possible, and learn about the influence of variables, such as gender and harvest time, on the production of C‐B1 by the tree. It was found that all studied trees tend to give higher C‐B1 percentages in the May‐July period, from 6 % to 18 %, and lower ones around March (spring) and November (fall), from 1 % to 8 %. In a general way, it also seems that the female trees tend to produce a bit more C‐B1 (from 2.8 % to 17.3 %) than male ones (from 1.7 % to 13.4 %). In addition, eight minor phenolic compounds [(−)‐ent‐catechin (1), (−)‐ent‐epicatechin‐(4α→8)‐ent‐epicatechin (2), (epi)catechin‐(4→8)‐(epi)afzelechin‐(4→8)‐(epi)catechin (3), (+)‐epiafzelechin‐(4β→8)‐epicatechin (4), (−)‐epicatechin (5), (−)‐afzelechin‐(4α→8)‐epicatechin (6), (epi)afzelechin‐(4→8)‐(epi)afzelechin‐(4→8)‐(epi)catechin (7) and (+)‐epicatechin‐(4β→8,2β→O‐7)‐epicatechin‐(4β→8)‐catechin (C‐D1)] were found and quantified in the ethyl acetate extract of the wood samples. The second aim of this work was to improve the recovery of C‐B1 from laurel wood. The use of the fast centrifugal partition chromatography (FCPC) technique has allowed for a recovery of 96 % of a technical‐grade C‐B1 (64 % in a previous protocol using conventional column chromatography on silica gel and size‐exclusion chromatography).

show abstract

Purification of Puerarin from Pueraria lobata by FCPC versus HSCCC Using Small-volume Columns

Cited by 6 publications

References 12 publications

Selectivity of Current Extraction Techniques for Flavonoids from Plant Materials

Selectivity of Current Extraction Techniques for Flavonoids from Plant Materials

Isolation of Mirificin and Other Bioactive Isoflavone Glycosides from the Kudzu Root Lyophilisate Using Centrifugal Partition and Flash Chromatographic Techniques

Recovery and Seasonal Variation of Cinnamtannin B‐1 from Laurel (Laurus nobilis L.) Pruning Wood Wastes

Contact Info

Product

Resources

About