Purification of several pectin methyltransferases from cell suspension cultures of flax (Linum usitatissimum L.)

Bourlard, Thierry; Bruyant-Vannier, Marie-Pierre; Schaumann, Annick; Bruyant, P.; Morvan, Claudine

doi:10.1016/s0764-4469(01)01309-9

Cited by 3 publications

(6 citation statements)

References 36 publications

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“…In vitro biochemical studies showing that UDP-GalA stimulates HG-MT activity in intact membrane vesicles (284,308) and that polygalacturonic and pectin can function in vitro as acceptors for HG-MT in detergent-permeabilized membranes support a model in which at least a small stretch of HG is synthesized prior to its methylation by HG-MT in the Golgi. The observation that some of the HG-MTs in detergent-permeabilized membranes from flax and soybean show a preference for partially esterified pectin (228,285,337) over polygalacturonic acid further suggest that multiple HG-MTs may exist that differ in their specificity for HG of differing degrees of methylation. The question of whether such HG-MTs are preferentially involved in the initial methylation of HG or in the methylation of more highly esterified HG remains to be resolved.…”

Section: Hg-methyltransferasementioning

confidence: 99%

“…Two apparent HG-MT isozymes, PMT5 and PMT7, were reported from flax (337) and efforts to purify these apparent isozymes resulted in the identification of an additional small polypeptide with HG-MT activity designated PMT18. The definitive identification of one or more of these polypeptides as HG-MT has not yet been reported.…”

Section: Hg-methyltransferasementioning

confidence: 99%

“…The definitive identification of one or more of these polypeptides as HG-MT has not yet been reported. Thus, the proposition that the 18-kDa protein is a subunit of the 40-and 110-kDa proteins (337) has not been substantiated.…”

Section: Hg-methyltransferasementioning

confidence: 99%

“…Hence, no wall-related acetyl-or methyltransferases have been functionally cloned. The methyltransferases (MetTs) and acetyltransferases (AceTs) generally utilize S-adenosyl-l-methionine (SAM) and acetyl-CoA as methyl and acetyl donors, respectively (229,285,335,337). A recent study in A. Orellana's laboratory led to the biochemical identification of a SAM transporter activity in the Golgi apparatus of pea (A. Orellana, personal communication).…”

Section: Nucleotide Sugarsmentioning

confidence: 99%

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Cell Wall Polysaccharide Synthesis

Mohnen

Bar‐Peled

Somerville

2008

Biomass Recalcitrance

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Section: Hg-methyltransferasementioning

confidence: 99%

Section: Hg-methyltransferasementioning

confidence: 99%

Section: Hg-methyltransferasementioning

confidence: 99%

Section: Nucleotide Sugarsmentioning

confidence: 99%

See 2 more Smart Citations

Cell Wall Polysaccharide Synthesis

Mohnen

Bar‐Peled

Somerville

2008

Biomass Recalcitrance

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“…The formed pectate is partly methylated. The enzymes that methylate pectin are not well characterised (GOUBET & MOHNEN 1999;BOURLAND et al 2001). They are typically referred to as pectin methyltransferases (or homogalacturonan methyltransferases, EC 2.1.1.6.x).…”

Section: Pectinmentioning

confidence: 99%

Biosynthesis of food constituents: Saccharides. 2. Polysaccharides - a review

Velı́šek¹,

Cejpek²

2005

Czech J. Food Sci.

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This review article gives a survey of the selected principal biosynthetic pathways that lead to the most important polysaccharides occurring in foods and in food raw materials and informs non-specialist readers about new scientific advances as reported in recently published papers. Subdivision of the topic is predominantly done via biosynthesis and includes reserve polysaccharides (starch and glycogen, fructans), plant cell wall polysaccharides (cellulose and callose, pectin), and animal polysaccharides (chitin and glycosaminoglycans). Extensively used are the reaction schemes, sequences, and mechanisms with the enzymes involved and detailed explanations using sound chemical principles and mechanisms.

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Plant cell and biotechnology studies in Linum usitatissimum – a review

Millam¹,

Obert

Preťová

2005

Plant Cell Tiss Organ Cult

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The species Linum usitatissimum (flax/linseed) has been the focus of a great deal of both basic and applied research effort in plant cell and biotechnology studies in recent years. In this review we consider applications of the techniques of plant biotechnology in this species under several distinct headings. Plant cell and tissue regeneration strategies and applications are discussed, and the applications of the techniques of somatic embryogenesis, protoplast isolation, culture and fusion and cell suspension cultures in this species are described. A major area of study is the use of anther and microspore culture where clear advantages to breeding programmes could be applied. In addition, embryo and ovary culture studies have resulted in significant findings. The more recent technologies of gene transfer and expression by genetic transformation are reviewed, and a section on strategies for improvements in technological quality is also included. Finally we propose conclusions and future prospects for this ancient, but still highly relevant crop.

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Purification of several pectin methyltransferases from cell suspension cultures of flax (Linum usitatissimum L.)

Cited by 3 publications

References 36 publications

Cell Wall Polysaccharide Synthesis

Cell Wall Polysaccharide Synthesis

Biosynthesis of food constituents: Saccharides. 2. Polysaccharides - a review

Plant cell and biotechnology studies in Linum usitatissimum – a review

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