Purification of spirosin from Lactobacillus reuteri DSM 20016 and partial characterization based on its amino acid sequences.

Yamato, Masayuki

doi:10.2323/jgam.44.173

J. Gen. Appl. Microbiol.

1998

DOI: 10.2323/jgam.44.173

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Purification of spirosin from Lactobacillus reuteri DSM 20016 and partial characterization based on its amino acid sequences.

Masayuki Yamato

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2003

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Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli

Echave¹,

Tamarit

Cabiscol

et al. 2003

Journal of Biological Chemistry

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Alcohol dehydrogenase E (AdhE) is an Fe-enzyme that, under anaerobic conditions, is involved in dissimilation of glucose. The enzyme is also present under aerobic conditions, its amount is about one-third and its activity is only one-tenth of the values observed under anaerobic conditions. Nevertheless, its function in the presence of oxygen remained ignored. The data presented in this paper led us to propose that the enzyme has a protective role against oxidative stress. Our results indicated that cells deleted in adhE gene could not grow aerobically in minimal media, were extremely sensitive to oxidative stress and showed division defects. In addition, compared with wild type, mutant cells displayed increased levels of internal peroxides (even higher than those found in a ⌬katG strain) and increased protein carbonyl content. This pleiotropic phenotype disappeared when the adhE gene was reintroduced into the defective strain. The purified enzyme was highly reactive with hydrogen peroxide (with a K i of 5 M), causing inactivation due to a metal-catalyzed oxidation reaction. It is possible to prevent this reactivity to hydrogen peroxide by zinc, which can replace the iron atom at the catalytic site of AdhE. This can also be achieved by addition of ZnSO 4 to cell cultures. In such conditions, addition of hydrogen peroxide resulted in reduced cell viability compared with that obtained without the Zn treatment. We therefore propose that AdhE acts as a H 2 O 2 scavenger in Escherichia coli cells grown under aerobic conditions.

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Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli

Echave¹,

Tamarit

Cabiscol

et al. 2003

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

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Purification of spirosin from Lactobacillus reuteri DSM 20016 and partial characterization based on its amino acid sequences.

Cited by 1 publication

References 12 publications

Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli

Novel Antioxidant Role of Alcohol Dehydrogenase E from Escherichia coli

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