Purification of synthetic peptides Immobilized metal ion affinity chromatography (IMAC)*

Lindeberg, Gunnar; Bennich, H.; Engström, Åke

doi:10.1111/j.1399-3011.1991.tb01436.x

Cited by 26 publications

(3 citation statements)

References 17 publications

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“…Phosphoproteome capture technology has been refined to increase the specificity, stability, and spatial resolution across different conditions. Advancements in Immobilized metal ion affinity chromatography (IMAC), Phos-tag and magnetic TiO 2 beads have been used over the years to refine the current phosphoproteome repertoire (Lindeberg et al, 1991;Lasonder et al, 2012). Understanding of phosphorylation events will reveal the cross-talks and complex networking pathways required for the parasite development.…”

Section: The Regulatory Role Of Kinases In Plasmodiummentioning

confidence: 99%

Decrypting the complexity of the human malaria parasite biology through systems biology approaches

Chahine

Roch

2022

Front. Syst. Biol.

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The human malaria parasite, Plasmodium falciparum, is a unicellular protozoan responsible for over half a million deaths annually. With a complex life cycle alternating between human and invertebrate hosts, this apicomplexan is notoriously adept at evading host immune responses and developing resistance to all clinically administered treatments. Advances in omics-based technologies, increased sensitivity of sequencing platforms and enhanced CRISPR based gene editing tools, have given researchers access to more in-depth and untapped information about this enigmatic micro-organism, a feat thought to be infeasible in the past decade. Here we discuss some of the most important scientific achievements made over the past few years with a focus on novel technologies and platforms that set the stage for subsequent discoveries. We also describe some of the systems-based methods applied to uncover gaps of knowledge left through single-omics applications with the hope that we will soon be able to overcome the spread of this life-threatening disease.

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Section: The Regulatory Role Of Kinases In Plasmodiummentioning

confidence: 99%

Decrypting the complexity of the human malaria parasite biology through systems biology approaches

Chahine

Roch

2022

Front. Syst. Biol.

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“…Originally, the technique was used for affinity purification of proteins. It is based on the interaction of peptides with an N-terminal metal-binding amino acid such as His, Trp, or Cys, especially when such residues are not present in other parts of the molecule [30].…”

Section: Immobilized Metal Ion Chromatographymentioning

confidence: 99%

Phosphoproteomics: Searching for a needle in a haystack

Tichý

Šalovská

Řehulka

et al. 2011

Journal of Proteomics

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“…Immobilised metal-affinity chromatography relies on the stable interactions between histidine and a metal such as nickel or zinc [45]. This interaction is extremely stable, owing to electron donor groups on the imidazole ring of histidine forming coordination bonds with the zinc.…”

Section: Affinity Tagsmentioning

confidence: 99%

Recombinant viral proteins for use in diagnostic ELISAs to detect virus infection

Spencer

Osorio

Hiscox

2007

Vaccine

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ELISAs provide a valuable tool in the detection and diagnosis of virus infection. The ability to produce recombinant viral proteins will ensure that future ELISAs are safe, specific and rapid. This latter point being the most crucial advantage in that even if a virus cannot be cultured, provided gene sequence is available, it is possible to rapidly respond to emerging viruses and new viral strains of existing pathogens. Indeed, ELISAs based on peptides (corresponding to epitopes) also hold great promise, as in this case no cloning or expression of a recombinant protein is required. Both recombinant protein and peptide based systems lend themselves to large scale production and purification. These approaches can also be used to distinguish recombinant vaccines from parental or wild type viruses.

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Purification of synthetic peptides Immobilized metal ion affinity chromatography (IMAC)*

Cited by 26 publications

References 17 publications

Decrypting the complexity of the human malaria parasite biology through systems biology approaches

Decrypting the complexity of the human malaria parasite biology through systems biology approaches

Phosphoproteomics: Searching for a needle in a haystack

Recombinant viral proteins for use in diagnostic ELISAs to detect virus infection

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