Purification of Synthetic Peptides Using a Catching Full-Length Sequence by Polymerization Approach

Abstract

During automated solid-phase peptide synthesis, failure sequences were capped with acetic anhydride. After synthesis, a polymerizable methacrylamide tag was attached to the full-length sequences. Peptide purification was then achieved by polymerizing the full-length sequences, washing away impurities, and cleaving the peptide product from the polymer.

Parallel, Large-Scale, and Long Synthetic Oligodeoxynucleotide Purification Using the Catching Full-Length Sequence by Polymerization Technique

“…Currently we are making efforts to identify a niche market for the catching by polymerization technique to enter the field of ODN production. We believe that the technique will be equally suitable for purification of RNA and other bio-oligomers, including peptides, 38 and we are also making efforts in those areas.…”

Controlled Conversion of Proteins into High-Molecular-Weight Peptides without Additives with High-Temperature Water and Fast Heating Rates

“…Although peptides can be produced from proteins with molecular biology, the techniques are highly specialized and require tedious synthesis, isolation, and purification steps often requiring large amounts of organic solvents that also generate much solid waste . Large-scale production of peptides on a 1000 kg-scale per year will require development of new approaches. , …”

“…7 Large-scale production of peptides on a 1000 kg-scale per year will require development of new approaches. 8,9 In wastewater treatment systems, much of the organic nitrogen that enters into the environment is due to proteins that are incompletely removed by the treatment process or are generated as a result of biological treatment. 10 Previously, we showed that organic nitrogen available in wastewater treatment effluents could be recycled as nutrients for microalgae cultivation with hydrothermal treatment and proposed that energy of the process could be obtained from low-quality (150 to 200) °C waste heat.…”

Direct Measurement of Rupture Force of Single Triazole Molecule by Atomic Force Microscope and Solid Phase Synthesis of Monodisperse Polyethylene Glycols

“…A showed 77% (PEG)20 and 23% (PEG)16 (Figure A.5). Although these results were significant because the longer PEGs could be easily separated from shorter ones using techniques involving tagging followed by chromatography, polymerization or extraction 34,36,[44][45][46][47] such studies had not been pursued. Instead, it came to our attention that the synthesis of pure (PEG)12 and its asymmetric derivatives using a convenient method without any chromatography is highly significant.…”

“…The product,28 which was contaminated with 3.17, was dried over fresh Drierite under high vacuum overnight, and was used directly for the solid phase PEG synthesis. The reaction was also performed at smaller scales and the product was purified with flash column chromatography and pure product was used for solid phase PEG synthesis.BnO(PEG)4:The procedure is similar to reported ones[40][41][42][43][44][45][46][47][48][49][50] with slight but important modifications. A suspension of NaH (60% in mineral oil, 8.3 g, 208 mmol, 1 equiv.)…”