Purification of therapeutic adenoviruses using laterally-fed membrane chromatography

Kawka, Karina; Madadkar, Pedram; Umatheva, Umatheny; Shoaebargh, Shabnam; Medina, Maria Fe C.; Lichty, Brian D.; Ghosh, Raja; Latulippe, David R.

doi:10.1016/j.memsci.2019.02.056

Cited by 11 publications

(4 citation statements)

References 18 publications

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“…In addition, LFMC working pressure is less than 200 kPa, no need for expensive high-pressure pumps for chromatographic systems [ 85 ]. LFMC has been proved to be a flow mode suitable for fast and efficient separation, and can in the maximum extent possible minimize sample dilution [ 5 , 83 , 86 ]. Such is the separation of PEGylated proteins and monoclonal antibody aggregates [ 87 ].…”

Section: Membrane Equipmentmentioning

confidence: 99%

Recent development and application of membrane chromatography

Chen

Cong

et al. 2022

Anal Bioanal Chem

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Membrane chromatography is mainly used for the separation and purification of proteins and biological macromolecules in the downstream processing process, also applications in sewage disposal. Membrane chromatography is recognized as an effective alternative to column chromatography because it significantly improves chromatography from affinity, hydrophobicity, and ion exchange; the development status of membrane chromatography in membrane matrix and membrane equipment is thoroughly discussed, and the applications of protein capture and intermediate purification, virus, monoclonal antibody purification, water treatment, and others are summarized. This review will provide value for the exploration and potential application of membrane chromatography. Graphical abstract

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Section: Membrane Equipmentmentioning

confidence: 99%

Recent development and application of membrane chromatography

Chen

Cong

et al. 2022

Anal Bioanal Chem

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“…The majority of resin-based chromatographic media, however, exhibit low viral particle dynamic binding capacities, since the pore diameter of the resins, usually less than 50–100 nm, may limit adsorption and pore diffusion of viral particles, whose diameters range from tens to hundreds of nanometers [ 19 ]. As a result, the capture of viral particles on resin beads are restricted mainly to the bead surface, resulting in poor ligand utilization [ 20 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…These separation media usually exhibit higher viral particle dynamic binding capacities than resins, especially at shorter residence times [ 28 , 29 ]. Some membrane adsorbers may be used as single-use disposable devices, eliminating costly and time-consuming cleaning-in-place and validation steps [ 20 , 25 , 30 ]. Because of these characteristics and ease of scale-up, membrane chromatography is ideally suited for the purification of viral particles [ 19 , 21 ].…”

Section: Introductionmentioning

confidence: 99%

“…McNally et al optimized the buffer conditions in a bind-and-elute anion-exchange step using Mustang Q membranes to recover retrovirus with high purity [ 31 ]. Ghosh et al demonstrated an efficient Ad5 separation using a laterally fed holder packed with Sartobind Q membranes, showing that this device outperformed a commercial radial flow system [ 20 ]. The cast membranes used in these studies, however, are not amenable to single-use applications [ 32 , 33 ].…”

Section: Introductionmentioning

confidence: 99%

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Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

et al. 2022

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The success of adeno-associated virus (AAV)-based therapeutics in gene therapy poses the need for rapid and efficient processes that can support the growing clinical demand. Nonwoven membranes represent an ideal tool for the future of virus purification: owing to their small fiber diameters and high porosity, they can operate at high flowrates while allowing full access to target viral particles without diffusional limitations. This study describes the development of nonwoven ion-exchange membrane adsorbents for the purification of AAV2 from an Sf9 cell lysate. A strong anion-exchange (AEX) membrane was developed by UV grafting glycidyl methacrylate on a polybutylene terephthalate nonwoven followed by functionalization with triethylamine (TEA), resulting in a quaternary amine ligand (AEX-TEA membrane). When operated in bind-and-elute mode at a pH higher than the pI of the capsids, this membrane exhibited a high AAV2 binding capacity (9.6 × 1013 vp·mL−1) at the residence time of 1 min, and outperformed commercial cast membranes by isolating AAV2 from an Sf9 lysate with high productivity (2.4 × 1013 capsids·mL−1·min−1) and logarithmic reduction value of host cell proteins (HCP LRV ~1.8). An iminodiacetic acid cation-exchange nonwoven (CEX-IDA membrane) was also prepared and utilized at a pH lower than the pI of capsids to purify AAV2 in a bind-and-elute mode, affording high capsid recovery and impurity removal by eluting with a salt gradient. To further increase purity, the CEX-IDA and AEX-TEA membranes were utilized in series to purify the AAV2 from the Sf9 cell lysate. This membrane-based chromatography process also achieved excellent DNA clearance and a recovery of infectivity higher that that reported using ion-exchange resin chromatography.

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Purification of monoclonal antibody using cation exchange z2 laterally-fed membrane chromatography – A potential alternative to protein A affinity chromatography

Roshankhah

Chen

et al. 2022

Biochemical Engineering Journal

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Purification of therapeutic adenoviruses using laterally-fed membrane chromatography

Cited by 11 publications

References 18 publications

Recent development and application of membrane chromatography

Recent development and application of membrane chromatography

Purification of Adeno-Associated Virus (AAV) Serotype 2 from Spodoptera frugiperda (Sf9) Lysate by Chromatographic Nonwoven Membranes

Purification of monoclonal antibody using cation exchange z2 laterally-fed membrane chromatography – A potential alternative to protein A affinity chromatography

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