Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Meera, Nair; Sandhu, Sardul Singh; Babbar, Anita

doi:10.1590/s2197-00252013000200006

Cited by 13 publications

(5 citation statements)

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“…They also recorded more than 35 per cent inhibition from wild Cicer, suggesting that a large proportion of HGP was insensitive to PIs from Cicer. Nair et al (2013) observed reduced larval weight and survival of final instar Helicoverpa larvae with the increased dose of trypsin inhibitor in the artificial diet. Similarly, recorded the lowest growth index for pulse beetle with increased level of protease inhibitors.…”

Section: Effect Of Host Chemical Factors On H Armigera Resistance In ...mentioning

confidence: 81%

Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Divija

Agnihotri

Reddy

2021

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Background: Chickpea pod borer (CPB), Helicoverpa armigera (Hübner) is a pest of great economic importance in chickpea and it is the major limiting factor in chickpea cultivation. In severe cases it causes about 75 to 90 per cent losses in seed yield, despite the application of insecticides. Therefore, development of a cost effective and an environmentally friendly approach like improvement of cultivars resistant to H. armigera is necessary for management of the pest in chickpea. Methods: Morphological and chemical components of host plant resistance in chickpea germplasm was assessed under field and laboratory conditions against pod borer, Helicoverpa armigera at hotspot Pantnagar, during rabi, 2017-18 using standard protocols. Result: Observations recorded revealed that germplasm ICC4484 recorded highest phenol (4.73 mg/g) and flavonoid (0.19 mg/g) content, whereas the maximum tannin, protein and trypsin content were recorded in ICC6263 (1.33 mg/g), ICC3137 (19.41 g/100 g of seeds) and ICC372351 (31.83 IU/g), respectively. Germplasm with higher phenol, tannin, flavonoid and trypsin inhibitor content recorded minimum per cent pod damage. Phenol, tannin, flavonoid, trypsin content showed negative correlation, while protein content showed positive correlation with the per cent pod damage by H. armigera.

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Section: Effect Of Host Chemical Factors On H Armigera Resistance In ...mentioning

confidence: 81%

Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Divija

Agnihotri

Reddy

2021

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“…The total proteins from the immature seeds of oviposited (T) and non-oviposited (C) plants were extracted using 20 mM phosphate buffer (pH 6.9). For TI activity, crude protein (50 μl) was mixed with 20 μl bovine pancreas trypsin (1 mg ml − 1 ) and incubated following the procedure described in Nair et al [ 100 ].…”

Section: Methodsmentioning

confidence: 99%

Bruchid beetle ovipositioning mediated defense responses in black gram pods

et al. 2021

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Background Black gram [Vigna mungo (L)] seeds are a rich source of digestible protein and dietary fibre, both for human and animal consumption. However, the quality and quantity of the Vigna seeds are severely affected by bruchid beetles during storage. Therefore, analyses of the expression of the bruchid induced transcript dynamics in black gram pods would be helpful to understand the underlying defense mechanism against bruchid oviposition. Results We used the RNAseq approach to survey the changes in transcript profile in the developing seeds of a moderately resistant cultivar IC-8219 against bruchid oviposition using a susceptible cultivar T-9 as a control. A total of 96,084,600 and 99,532,488 clean reads were generated from eight (4 each) samples of IC-8219 and T-9 cultivar, respectively. Based on the BLASTX search against the NR database, 32,584 CDSs were generated of which 31,817 CDSs were significantly similar to Vigna radiata, a close relative of Vigna mungo. The IC-8219 cultivar had 630 significantly differentially expressed genes (DEGs) of which 304 and 326 genes up and down-regulated, respectively. However, in the T-9 cultivar, only 168 DEGs were identified of which 142 and 26 genes up and down-regulated, respectively. The expression analyses of 10 DEGs by qPCR confirmed the accuracy of the RNA-Seq data. Gene Ontology and KEGG pathway analyses helped us to better understand the role of these DEGs in oviposition mediated defense response of black gram. In both the cultivars, the most significant transcriptomic changes in response to the oviposition were related to the induction of defense response genes, transcription factors, secondary metabolites, enzyme inhibitors, and signal transduction pathways. It appears that the bruchid ovipositioning mediated defense response in black gram is induced by SA signaling pathways and defense genes such as defensin, genes for secondary metabolites, and enzyme inhibitors could be potential candidates for resistance to bruchids. Conclusion We generated a transcript profile of immature black gram pods upon bruchid ovipositioning by de novo assembly and studied the underlying defense mechanism of a moderately resistant cultivar.

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“…The TIs in Cicer arietinum (L.) need more attention in respect of huge infestations of pests on this crop. In one study, TI from chickpea seeds was characterized and demonstrated for mortality and dose‐dependent reduction in the larval weight of the fifth instar against Helicoverpa armigera (Hubner) 57 . C. arietinum (L.) seeds have Kunitz‐type serine PIs that have shown antitrypsin, antichymotrypsin and antimicrobial effects on Fusarium oxysporum f. sp.…”

Section: Endogenous Ppismentioning

confidence: 99%

Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

Pandey

Yadav

Sanyal

2021

Pest Management Science

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In the arsenal of plant defense, protease inhibitors (PIs) are well-designed defensive products to counter field pests. PIs are produced in plant tissues by means of 'stable defense metabolite' and triggered on demand as the perception of the signal and well established as a part of plant active defense. PIs have been utilized for approximately four decades, initially as a gene-alone approach that was later replaced by multiple gene pyramiding/gene stacking due to insect adaptability towards the PI alone. By considering the adaptive responses of the pest to the single insecticidal gene, the concept of gene pyramiding gained continuous appreciation for the development of transgenic crops to deal with co-evolving pests. Gene pyramiding approaches are executed to bypass the insectʼs adaptive responses against PIs. Stacking PIs with additional insecticidal proteins, plastid engineering, recombinant proteinase inhibitors, RNAi-based methods and CRISPR/Cas9-mediated genome editing are the advanced tools and methods for next-generation pest management. Undoubtedly, the domain associated with the mechanism of PIs in the course of plant-pest interactions will occupy a central role for the advancement of more efficient and sustainable pest control strategies.

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Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Cited by 13 publications

References 40 publications

Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Bruchid beetle ovipositioning mediated defense responses in black gram pods

Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

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Purification of trypsin inhibitor from seeds of Cicer arietinum (L.) and its insecticidal potential against Helicoverpa armigera (Hübner)

Cited by 13 publications

References 40 publications

​​Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

​​Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Bruchid beetle ovipositioning mediated defense responses in black gram pods

Evaluating the pesticidal impact of plant protease inhibitors: lethal weaponry in the co‐evolutionary battle

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Product

Resources

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Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm

Morphological and Chemical Components of Resistance to Pod Borer, Helicoverpa armigera (Hübner) in Chickpea Germplasm