Purification of two astroglial growth factors from bovine brain

Pettmann, Brigitte; Weibel, M.; Sensenbrenner, M.; Labourdette, G.

doi:10.1016/0014-5793(85)80851-6

Cited by 241 publications

(81 citation statements)

References 18 publications

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“…The results presented here are certainly compatible with the suggestion that basic FGF may have a multifunctional role in promoting glial hypertrophy and proliferation 33 and in stimulating angiogenesis 21 . At 3 days, many of the basic FGF-immunopositive cells are immunopositive for an antigen (MAC-1) common to cells of the monocyte-macrophage lineage, including ameboid microglia.…”

Section: Discussionsupporting

confidence: 90%

“…These cells which acquire characteristics of microglia and macro-phages 1 may thus contribute basic FGF-like activities. First and foremost, they stimulate angiogenesis 6 and glial proliferation 33,34 . Because activated macrophages contain basic FGF 3 , it is thus possible that, as part of the early phase of the regenerative process, basic FGF is available locally from monocytes and/or macrophages and helps initiate the response to CNS injury.…”

Section: Discussionmentioning

confidence: 99%

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Localization of basic fibroblast growth factor and its mRNA after CNS injury

1991

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Basic fibroblast growth factor (FGF) mRNA is increased 4 h after cortical brain injury. In situ hybridization reveals that the increased mRNA persists for at least 2 weeks and that, in areas adjacent and ipsilateral to the lesion, the expression of basic FGF mRNA is also modified. As an example, at three days distal from the lesion, mRNA can be detected in ependymal cells of the lateral ventricle and in selected cells of the hippocampus and cortex. Endothelial cells also synthesize basic FGF mRNA. The increase in basic FGF mRNA is paralleled by similar changes in the localization of the basic FGF protein. Both the intensity and number of cells which stain for basic FGF are increased when they are compared to staining in either the contralateral side or to comparable areas of unlesioned brains. The pattern of mRNA expression is similar from 4 hours to 14 days. Early in the response (4 h to 3 days) on the border of the lesion, the presence of basic FGF is most obvious within the MAC-1-immunopositive population (macrophages and/or microglia). From 7 days to 2 weeks, there has been extensive hypertrophy of the reactive astrocytes which stain intensely for anti-basic FGF(1-24). We conclude that there is increased basic FGF as a function of injury to the CNS. In view of the observation that it is an early and persistent response, the possibility that it plays multiple functions in the regenerative capacity of the CNS is discussed.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Localization of basic fibroblast growth factor and its mRNA after CNS injury

1991

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“…In vitro, bFGF promotes the survival and out growth of a wide variety of neurons from embryonic rat brain and supports the survival and proliferation of brain glia and capillary endothelial cells (Gospo darowicz et al, 1986a;Pettman et al, 1985;Wa licke, 1988). In the intact brain, bFGF is localized to neurons and glial cells, whereas the high-affinity bFGF receptor (fig) is widely localized on both neu rons as well as non-neuronal cells (Emoto et al, 1989;Finklestein et al, 1988;Gomez-Pinilla et al, 1992;Woodward et al, 1992).…”

mentioning

confidence: 99%

Basic Fibroblast Growth Factor Dilates Rat Pial Arterioles

Rosenblatt

Irikura

Caday

et al. 1994

J Cereb Blood Flow Metab

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Summary: Basic fibroblast growth factor (bFGF) is a polypeptide that promotes the survival and differentiation of brain neurons, glia, and endothelial cells. It has been shown recently that intravenously administered bFGF lowers blood pressure by systemic vasodilation; this ef fect is mediated, in part, by nitric oxide (NO)-dependent mechanisms. In the current study, we directly evaluated the effect of bFGF on pial arterioles of pentobarbital anesthetized Sprague-Dawley rats (n = 18) using the closed cranial window technique. Basic FGF (5-200 ng/ ml) produced dose-dependent vasodilation; maximal ves sel diameter (-120% of control) was reached at 100 ng/ Basic fibroblast growth factor (bFGF) is an 18-kDa polypeptide found within the mammalian brain with potent multipotential trophic effects on brain cells. In vitro, bFGF promotes the survival and out growth of a wide variety of neurons from embryonic rat brain and supports the survival and proliferation of brain glia and capillary endothelial cells (Gospo darowicz et al., 1986a;Pettman et al., 1985; Wa licke, 1988). In the intact brain, bFGF is localized to neurons and glial cells, whereas the high-affinity bFGF receptor (fig) is widely localized on both neu rons as well as non-neuronal cells (Emoto et al., 1989;Finklestein et al., 1988;Gomez-Pinilla et al., 1992;Woodward et al., 1992). In particular, the density of bFGF receptors is high in vascular brain structures (including circum ventricular organs and choroid plexus), most likely due to their localization Abbreviations used: ANOVA, analysis of variance; bFGF, ba sic fibroblast growth factor; BSA, bovine serum albumin; CGRP, calcitonin gene-related peptide; EAA, excitatory amino acids; L-NAME, ,vo-nitro-L-arginine methyl ester; PBS, phosphate buffered saline. 70ml. No vasodilation was found when bFGF was heat in activated, or preincubated with blocking antibody. Moreover, bFGF-induced vasodilation was attenuated by coadministration of the NO synthase inhibitor �-nitro L-arginine methyl ester (L-NAME), consistent with an NO-dependent mechanism. These results suggest that bFGF may play an important role in the regulation of cerebrovascular tone and cerebral blood flow.

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“…FGFs exhibit trophic activity for glial, neuronal and endothelial cells in vitro (Morrison and Vellis 1981;Pettman et al 1985; Walicke and Baird 1988) and stimulate angiogenesis in vivo (Gospodarowicz et al 1978). They comprise two major families: acidic (Thomas et al 1980) and basic (Gospodarowicz et al 1975; Lemmon et al 1982), which were purified and their amino acid sequences determined (Esch et al 1985; Gimenez-Gallego et al 1985).…”

mentioning

confidence: 99%