Purification of urokinase by combined cation exchanger and affinity chromatographic cartridges

Hou, Kenneth C.; Zaniewski, Richard P.

doi:10.1016/s0378-4347(00)83406-4

Cited by 26 publications

(12 citation statements)

References 22 publications

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“…Th ere are numerous papers on the purifi cation of gamma globulin fraction, h-serum-albumin, and various proteins purifi cations methods. An interesting application of affi nity membranes was reported by Hou and Zaniewski [40] who purifi ed crude urokinase from human urine through a multistep process to prepare this plasminogen-activating enzyme for therapeutic use. Th e membranes used in this work were cation exchange matrices prepared on cellulose fi bers.…”

Section: Affi Nity Membranes For Protein Separationmentioning

confidence: 99%

Molecular Recognition‐Driven Membrane Processes

Donato¹,

Mazzei²,

Algieri³

et al. 2014

Smart Membranes and Sensors

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High-precision, selective and effi cient systems are strongly demanded in all productive sectors, including chemical manufacturing, pharmaceutical, medical treatment, food, energy, diagnostic, analytical tools. Precision separation, conversion, formulation at molecular level may rely on various mechanisms and properties, such as molecular sieving, electrical charge, chemical solubility, molecular recognition. Membrane operation can promote mass transport, separation, conversion, formulation on the basis of the mentioned mechanisms. Biomimic and biohybrid funtionalised membranes are particularly suitable for separations based on molecular recognition.Membranes can be formed by polymers or monolythes bearing the (biomimic) recognition sites. In alternative, biomimic, biomolecules and recognition sites can be loaded on a previously formed membrane support. In such cases, surface functionalization methods can improve the loading effi ciency. Molecularly imprinted membranes; membranes bearing affi nity ligands (Antigen-Antibody, avidin/-ligand, DNA-protein, sugar-lectin, RNA-ribosome,…); membranes functionalized with zeolite and nanoparticles; membranes with immobilized enantioselective enzyme for kinetic resolutions, are among examples of selective and effi cient operations driven by molecular recognition. Th is is the mechanism by means of which molecules are able to identify each other using non-covalent -(hydrogen bonding, metal coordination, hydrophobic forces, van der Waals forces, π-π interactions and electrostatic eff ects) and covalent interactions.

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Section: Affi Nity Membranes For Protein Separationmentioning

confidence: 99%

Molecular Recognition‐Driven Membrane Processes

Donato¹,

Mazzei²,

Algieri³

et al. 2014

Smart Membranes and Sensors

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“…Urokinase was purified with the zinc salt of amindeoxycellulose derivatives [68]. The 2-aminodeoxycelluloses were patented for their use in treatment of neurological disorders [69] and chronic inflammatory diseases such as colitis and arthritis [70]. The reaction products of chlorodeoxycellulose with substituted hydroxylamines followed by etherification with ClCH 2 COONa, produce betaine structures that were used as moisturizers in cosmetic and cleaning products [71].…”

Section: Biologicalmentioning

confidence: 99%

Deoxycelluloses and related structures

Vigo¹,

Sachinvala²

1999

Polym. Adv. Technol.

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The syntheses of various deoxycelluloses and related structures (cellulosenes and anhydrocelluloses) from the mid‐1920s to the present are critically reviewed. General synthetic strategies to prepare deoxycelluloses include nucleophilic displacement of good leaving groups. Distinctions are made between reaction of cellulosics under homogeneous and heterogeneous conditions. Recent advances in the preparation of halodeoxycelluloses have led to high degree of substitution fluorodeoxycelluloses and bromodeoxycelluloses. Applications for the deoxycelluloses are numerous and characterized by biological, chemical or physical end uses.

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“…The immobilized IDA Cu 2 chelate membrane was a composite of cellulose with grafted acrylic polymers prepared by a modified method of Hou and Zaniewski (1990). Here the cellulose is short cotton fiber instead of wood fiber.…”

Section: Membrane and Devicesmentioning

confidence: 99%