Purification of wheat grain fructans from wheat bran

Verspreet, Joran; Dornez, Emmie; Delcour, Jan A.; Harrison, S. P.

doi:10.1016/j.jcs.2015.06.013

Cited by 20 publications

(7 citation statements)

References 13 publications

(15 reference statements)

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“…To evaluate the alteration of the fructan profile ranging from DP 4–16, the fingerprint before and after fermentation is illustrated. The DP of fructans was estimated based on their retention times according to Verspreet et al [ 34 ]. Due to the removal of starch-based dextrins by amylases, all detected peaks can be assigned to fructans.…”

Section: Resultsmentioning

confidence: 99%

Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs

et al. 2018

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A diet low in fermentable oligosaccharides, disaccharides, monosaccharides and, polyols (FODMAPs) is recommended for people affected by irritable bowel syndrome (IBS) and non-coeliac wheat sensitivity (NCWS) in order to reduce symptoms. Therefore, the aim of this study was to evaluate the impact of 13 sourdough-related yeasts on FODMAP degradation, especially fructans. First, a model system containing a typical wheat carbohydrate profile was applied to evaluate the growth rate of each yeast strain. Additionally, changes in the sugar composition, for up to four days, were monitored by high-pressure anion-exchange chromatography (HPAEC). A more realistic approach with a wheat flour suspension was used to characterize CO2 production according to the Einhorn method. The reduction of the total fructans was analyzed using an enzymatic method. Furthermore, a fingerprint of the present fructans with different degrees of polymerization was analyzed by HPAEC. The results revealed strong differences in the examined yeast strains’ ability to degrade fructans, in both the model system and wheat flour. Overall, Saccharomyces cerevisiae isolated from Austrian traditional sourdough showed the highest degree of degradation of the total fructan content and the highest gas building capacity, followed by Torulaspora delbrueckii. Hence, this study provides novel knowledge about the FODMAP conversion of yeast strains.

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Section: Resultsmentioning

confidence: 99%

Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs

et al. 2018

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“…Further, inulin and neoseries-type fructans were isolated from onions and barley grain [ 35 ]. In addition, fructan-related chromatographic peaks were identified based on fructanase digestion and mild acid hydrolysis [ 35 ] as well as based on available literature [ 9 , 36 , 37 , 38 ]. A total of 57 peaks have been annotated ( Table 1 ).…”

Section: Methodsmentioning

confidence: 99%

Fructans Are Differentially Distributed in Root Tissues of Asparagus

Witzel

Matros

2020

Cells

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Inulin- and neoseries-type fructans [fructooligosaccharides (FOS) and fructopolysaccharides] accumulate in storage roots of asparagus (Asparagus officinalis L.), which continue to grow throughout the lifespan of this perennial plant. However, little is known about the storage of fructans at the spatial level in planta, and the degree of control by the plant is largely uncertain. We have utilized mass spectrometry imaging (MSI) to resolve FOS distribution patterns in asparagus roots (inner, middle, and outer tissues). Fructan and proteome profiling were further applied to validate the differential abundance of various fructan structures and to correlate observed tissue-specific metabolite patterns with the abundance of related fructan biosynthesis enzymes. Our data revealed an increased abundance of FOS with higher degree of polymerization (DP > 5) and of fructopolysaccharides (DP11 to DP17) towards the inner root tissues. Three isoforms of fructan:fructan 6G-fructosyltransferase (6G-FFT), forming 6G-kestose with a β (2–6) linkage using sucrose as receptor and 1-kestose as donor, were similarly detected in all three root tissues. In contrast, one ß-fructofuranosidase, which likely exhibits fructan:fructan 1-fructosyltransferase (1-FFT) activity, showed very high abundance in the inner tissues and lower levels in the outer tissues. We concluded a tight induction of the biosynthesis of fructans with DP > 5, following a gradient from the outer root cortex to the inner vascular tissues, which also correlates with high levels of sucrose metabolism in inner tissues, observed in our study.

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“…Graminans were extracted from wheat bran (Triticum aestivum) according to Verspreet et al [97]. After water extraction and subsequent EtOH (66%) precipitation, a lyophilized powdered sample was incubated with an α-galactosidase to degrade raffinose.…”

Section: Fructan Priming Compound Preparationsmentioning

confidence: 99%

Sweet Immunity Aspects during Levan Oligosaccharide-Mediated Priming in Rocket against Botrytis cinerea

Versluys

Ende

2022

Biomolecules

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New strategies are required for crop protection against biotic stress. Naturally derived molecules, including carbohydrates such as fructans, can be used in priming or defense stimulation. Rocket (Eruca sativa) is an important leafy vegetable and a good source of antioxidants. Here, we tested the efficacy of fructan-induced immunity in the Botrytis cinerea pathosystem. Different fructan types of plant and microbial origin were considered and changes in sugar dynamics were analyzed. Immune resistance increased significantly after priming with natural and sulfated levan oligosaccharides (LOS). No clear positive effects were observed for fructo-oligosaccharides (FOS), inulin or branched-type fructans. Only sulfated LOS induced a direct ROS burst, typical for elicitors, while LOS behaved as a genuine priming compound. Total leaf sugar levels increased significantly both after LOS priming and subsequent infection. Intriguingly, apoplastic sugar levels temporarily increased after LOS priming but not after infection. We followed LOS and small soluble sugar dynamics in the apoplast as a function of time and found a temporal peak in small soluble sugar levels. Although similar dynamics were also found with inulin-type FOS, increased Glc and FOS levels may benefit B. cinerea. During LOS priming, LOS- and/or Glc-dependent signaling may induce downstream sweet immunity responses.

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Purification of wheat grain fructans from wheat bran

Cited by 20 publications

References 13 publications

Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs

Applicability of Yeast Fermentation to Reduce Fructans and Other FODMAPs

Fructans Are Differentially Distributed in Root Tissues of Asparagus

Sweet Immunity Aspects during Levan Oligosaccharide-Mediated Priming in Rocket against Botrytis cinerea

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